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Enhanced Lignin Degradation in Tobacco Stalk Composting with Inoculation of White-Rot Fungi Trametes hirsuta and Pleurotus ostreatus

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Abstract

Purpose

Tobacco stalk contained large amount of lignin, which is resistant to microbial degradation and limit the composting efficiency during composting. The purpose of this study is to enhance the lignin degradation efficiency, and consequently improve the composting efficiency and product quality.

Methods

Lignin degrading microorganisms (LDMs) were screened and inoculated to tobacco stalk compost, lignin degradation enzyme activities and lignin degradation rate were measured to evaluate the lignin degradation efficiency, other physico-chemical characters were determined as well to evaluate the composting efficiency and product quality.

Results

Two white-rot fungi Trametes hirsuta S13 and Pleurotus ostreatus S18 were screened as LDM according to their high lignin degradation efficiency. After inoculation, lignin peroxidase, manganese peroxidase and laccase were raised from (U g−1) 105.9, 372.9 and 460.68 to 374.7, 1095.6 and 1420.8, respectively. Consequently, lignin degradation rate increased nearly 2-fold (from 23.7 to 41.1%). In addition, organic matter degradation rate was increased from 26.5 to 31.2% with the addition of fungal inoculants. Degree of polymerization, humification index and germination index were also elevated, from 2.0, 17.8% and 108.3% to 3.5, 24.2% and 123.8%, respectively.

Conclusions

In this study, T. hirsuta S13 and P. ostreatus S18 were used as LDM and inoculated to tobacco stalk composting. After inoculation, the lignin degradation rate was increased, with the composting efficiency and the quality of composting product was improved as well.

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Acknowledgements

This work was supported by the National Program on Key Basic Research Project (973 Program, No. 2015CB150505), the Science and Technology Program of Wuhan (20160201010086), and the Key Project of China National Tobacco Corporation Hubei Company.

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Author notes

  1. Liang Yang and Honglun Yuan contributed equally to this work.

Authors and Affiliations

  1. Department of Brewing Engineering, Moutai College, Renhuai, 564501, China

    Liang Yang

  2. Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Environmental Microbial Engineering and Technology Research Center of Hubei Province, College of Life Sciences, Hubei University, No. 368 Youyi Avenue, Wuchang District, Wuhan, 430062, Hubei, China

    Yong Yang, Shouwen Chen & Xin Ma

  3. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Liang Yang, Honglun Yuan, Xuetuan Wei & Shouwen Chen

  4. Tobacco Research Institute of Hubei Province, Wuhan, 430062, China

    Changjun Wang & Jun Yu

  5. Enshi Tobacco Company of Hubei Province, Enshi, 445000, China

    Rui Wang

  6. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Xuetuan Wei

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  1. Liang Yang
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Yang, L., Yuan, H., Yang, Y. et al. Enhanced Lignin Degradation in Tobacco Stalk Composting with Inoculation of White-Rot Fungi Trametes hirsuta and Pleurotus ostreatus. Waste Biomass Valor 11, 3525–3535 (2020). https://doi.org/10.1007/s12649-019-00692-z

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