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Detection of Theileria annulata carriers in Holstein–Friesian (Bos taurus taurus) and Sistani (Bos taurus indicus) cattle breeds by polymerase chain reaction in Sistan region, Iran

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Abstract

Theileria annulata is common in tropical and subtropical regions especially in Iran and causes great economic losses in cattle industry. In Iran the epidemiological aspects of bovine theileriosis in different breeds of cattle is poorly understood. The aim of present study is comparison of the number of T. annulata carriers in the two major cattle breeds (Holstein–Friesian and Sistani) in Sistan of Iran by giemsa and polymerase chain reaction (PCR) methods. During winter 2013, 160 native cattle, from the two major breeds in Sistan, with the mean age of more than one year and without typical clinical symptoms of theileriosis were selected. At first, a thin layer smear was held from their ear sublime vein blood for Giemsa staining method. In order to do PCR assay, jugular vein blood sample of each cow was taken. The PCR employs primers specific for the 721-bp gene fragment encoding the 30-kDa major merozoite surface antigen of T. annulata. By PCR method, 38 (47.5 %) Holstein blood samples and 22 (27.5 %) Sistani blood samples had DNA of T. annulata and considered positive (The correlation was significant at values of P < 0.05). By checking 160 blood smears with light microscope and lens × 100, only 10 samples (6.25 %) were positive for T. annulata. Statistical comparison between PCR and smear method showed that the PCR method is more sensitive and accurate in comparison to Giemsa staining method to diagnose the asymptomatic carriers of T. annulata.

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References

  • Aktas M, Dumanli N, Cetinkaya B et al (2002) Field evaluation of PCR in detecting Theileria annulata infection in cattle in eastern Turkey. Vet rec 150:548–549

    Article  CAS  PubMed  Google Scholar 

  • Almeria S, Castella J, Ferrer D et al (2001) Bovine piroplasms in Minorca (Balearic Islands, Spain): a comparison of PCR based and light microscopy detection. Vet Parasitol 99:249–259

    Article  CAS  PubMed  Google Scholar 

  • Ananda K, D’Souza P, Puttalashmamma G (2009) Prevalence of haemoprotozoan diseases in crossbred cattle in Bangalore North. Vet World 2(1):15–16

    Article  Google Scholar 

  • Azizi H, Shiran B, Dehkordi A et al (2008) Detection of Theileria annulata by PCR and its comparison with smear method in native carrier cows. Biotechnology 7(3):574–577

    Article  CAS  Google Scholar 

  • Barker R, Banchongaksorn T, Courval J et al (1992) A simple method to detect Plasmodium falciparum directly from blood samples using the Polymerase chain reaction. J Trop Med Hyg 46(4):416–426

    Google Scholar 

  • Brown CGD (1990) Control of tropical theileriosis (Theileria annulata infection of cattle). Parasitologia 32:23–31

    CAS  Google Scholar 

  • Burridge M, Brown C, Kimber C (1974) Theileria annulata: cross reactions between a cell culture schizont antigen and antigens of East African Theileria species in the indirect fluorescent antibody test. Exp Parasitol 35:374–379

    Article  CAS  PubMed  Google Scholar 

  • d’Oliveira C, van der Weide M, Habela M et al (1995) Detection of Theileria annulata in blood samples of carrier cattle by PCR. J Clin Microbiol 33(10):2665–2669

    PubMed  PubMed Central  Google Scholar 

  • d’Oliveira C, van der Weide M, Jacquiet P et al (1997) Detection of Theileria annulata by PCR in ticks (Acari: Ixodidae) collected from cattle in Mauritania. Exp Appl Acarol 21:279–291

    Article  PubMed  Google Scholar 

  • Darghouth M (2008) Review on the experience with live attenuated vaccines against tropical theileriosis in Tunisia: considerations for the present and implications for the future. Vaccine 26:G4–G10

    Article  CAS  PubMed  Google Scholar 

  • Darghouth M, Bouattour A, Ben-Milad L et al (1996) Epidemiology of tropical theileriosis (Theileria annulata infection of cattle) in an endemic region of Tunisia: characterization of endemicity states. Vet Parasitol 65:199–211

    Article  CAS  PubMed  Google Scholar 

  • Dolan TT (1989) Theileriosis: a comprehensive review. Rev Sci Tech Off Int Epizoot 8:11–36

    Article  Google Scholar 

  • Dumanli N, Aktas M, Cetinkaya B et al (2005) Prevalence and distribution of tropical theileriosis in eastern Turkey. Vet Parasitol 127(1):9–15

    Article  CAS  PubMed  Google Scholar 

  • Eamens G, Bailey G, Jenkins C et al (2013) Significance of Theileria orientalis types in individual affected beef herds in New South Wales on clinical, smear and PCR findings. Vet Parasitol 196:96–105

    Article  PubMed  Google Scholar 

  • Glass E, Preston P, Springbett A et al (2005) Bos taurus and Bos indicus (Sahiwal) calves respond differently to infection with theileria annulata and produce markedly different levels of acute phase proteins. Int J Parasitol 35(3):337–347

    Article  CAS  PubMed  Google Scholar 

  • Gubbels J, Katzer F, Hide G et al (2000) Generation of a mosaic pattern of diversity in the major merozoites piroplasm surface antigen of Theileria annulata. Mol Biochem Parasitol 110:23–32

    Article  CAS  PubMed  Google Scholar 

  • Hashemi-Fesharaki R (1988) Control of Theileria annulata in Iran. Parasitol Today 4(2):36–40

    Article  Google Scholar 

  • Heidarpour-Bami M, Haddadzadeh H, Kazemi B et al (2009) Molecular identification of ovine Theileria species by a new PCR-RFLP method. Vet Parasitol 161:171–177

    Article  CAS  PubMed  Google Scholar 

  • Hoghooghi-Rad N, Ghaemi P, Shayan P et al (2011) Detection of native carrier cattle infected with Theileria annulata by semi-nested PCR and smear method in Golestan province of Iran. World Appl Sci J 12(3):317–323

    CAS  Google Scholar 

  • Katzer F, McKellar S, Kirvar E et al (1998) Polygenetic analysis of Theileria and Babesia equi in relation to the establishment of parasite populations within novel host species and the development of diagnostic tests. Mol Biochem Parasitol 95:33–44

    Article  CAS  PubMed  Google Scholar 

  • Kelly WR (1974) Veterinary Clinical Diagnosis. Bailliere Tindall, London

    Google Scholar 

  • Kirvar E, Ilhan T, Katzer F et al (1998) Detection of Theileria lestoquardi (hirci) in ticks, sheep, goats using polymerase chain reaction. Ann NY Acad Sci 849:52–62

    Article  CAS  PubMed  Google Scholar 

  • Kirvar E, Ilhan T, Katzer F et al (2000) Detection of Theileria annulata in cattle and vector ticks by PCR using the Tams1 gene sequences. Parasitology 120:245–254

    Article  CAS  PubMed  Google Scholar 

  • Kundave VR, Patel AK, Patel PV et al (2013) Detection of theileriosis in cattle and buffaloes by polymerase chain reaction. J Parasit Dis. doi:10.1007/s12639-013-0386-2

    PubMed  PubMed Central  Google Scholar 

  • Leemans I, Brown D, Hooshmand-Rad P (1999) Infectivity and cross immunity studies of Theileria lestoquardi and Theileria annulata in sheep and cattle I. In vivo response. Vet Parasitol 82:179–192

    Article  CAS  PubMed  Google Scholar 

  • Martin-Sanchez J, Viseras J, Adroher FJ et al (1999) Nested polymerase chain reaction for detection of Theileria annulata and comparison with conventional diagnostic techniques: its use in epidemiology studies. Parasitol Res 85:243–245

    Article  CAS  PubMed  Google Scholar 

  • Mehlhorn H, Schein E (1984) The Piroplasms: life cycle and sexual stages. Adv Parasitol 23:37–103

    Article  CAS  PubMed  Google Scholar 

  • Mohammad Al-Saeed AT, Omer LT, Abdo J et al (2010) Epidemiological studies on tropical theileriosis (Theileria annulata infection of cattle) in Kurdistan region, Iraq. Parasitol Res 106:403–407

    Article  PubMed  Google Scholar 

  • Morzaria SP, Musoke AJ, Latif AA (1988) Recognition of Theileria parva antigens by field sera from Rusinga Islands. Kenya. Kenya Vet 12:8

    Google Scholar 

  • Nazifi S, Razavi M, Reiszadeh M et al (2010) Evaluation of the resistance of indigenous Iranian cattle to Theileria annulata compared with Holstein cattle by measurement of acute phase proteins. Comp Clin Pathol 19:155–161

    Article  Google Scholar 

  • Neitz WO (1957) Theileriosis, gonderioses and cytauxzoonoses. Onderstepoort J Vet Res 27:275–430

    Google Scholar 

  • Noaman V (2014) Comparison of molecular and microscopic technique for detection of Theileria spp. in carrier cattle. J Parasit Dis 38:64–67

    Article  PubMed  Google Scholar 

  • Sayin F, Karaer Z, Dincer S et al (2003) Studies on the epidemiology of tropical theileriosis (Theileria annulata infection) in cattle in central Anatolia. Turkey. Trop Anim Health Prod 35(6):521–539

    Article  CAS  PubMed  Google Scholar 

  • Schnittger L, Rodriguez A, Florin-Christensen A (2012) Babesia, a world emerging. Infect Genet Evol 12:1788–1809

    Article  PubMed  Google Scholar 

  • Shayan P, Rahbari S (2005) Simultaneous differentiation between Theileria spp. and Babesia spp. on stained blood smear using PCR. Parasitol Res 97(4):281–286

    Article  PubMed  Google Scholar 

  • Spickett AM, De Klerk D, Enslin CB et al (1989) Resistance of Nguni, Bonsmara and Hereford cattle to ticks in a Bushveld region of South Africa. Onderstepoort J Vet Res 56:245–250

    CAS  PubMed  Google Scholar 

  • Terada Y, Ishida M, Yamanaka H (1995) Resistibility to Theileria sergenti infection in Holstein and Japanese Black cattle. J Vet Med Sci 57(6):1003–1006

    Article  CAS  PubMed  Google Scholar 

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The authors declare that they have no conflict of interests.

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Authors and Affiliations

  1. Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

    Hamidreza Majidiani, Reza Nabavi & Maryam Ganjali

  2. Department of Food Hygiene & Quality Control, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

    Dariush Saadati

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  1. Hamidreza Majidiani
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  2. Reza Nabavi
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  3. Maryam Ganjali
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  4. Dariush Saadati
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Correspondence to Reza Nabavi.

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Majidiani, H., Nabavi, R., Ganjali, M. et al. Detection of Theileria annulata carriers in Holstein–Friesian (Bos taurus taurus) and Sistani (Bos taurus indicus) cattle breeds by polymerase chain reaction in Sistan region, Iran. J Parasit Dis 40, 1184–1188 (2016). https://doi.org/10.1007/s12639-015-0646-4

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