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Pathobiology of human RH strain induced experimental toxoplasmosis in murine model

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Abstract

Of late, toxoplasmosis has gained immense importance as an opportunist parasite in immunocompromised patients. In immunocompromised subjects, the disease is supposed to occur in acute form and causes acute toxoplasmic encephalitis. However, the exact pathogenesis of other vital organs, particularly in acute form of infection, is still a matter of debate. Therefore, an attempt was made to study the pathogenesis of acute form of toxoplasmosis using cryopreserved human RH strain of the parasite in murine models. For this, 100 tachyzoites were given to individual mice and upon the setup of acute form of infection, the mice were euthanized and the organs were processed for histopathology. Histopathology revealed tachyzoites in liver only while severe necrosis due to multiplication of tachyzoites were visible in liver, spleen, lungs and brain. Kidneys and heart appeared more or less normal. Finally, the pathology of disease in these organs is described in detail. The present research has generated some vital information regarding necrotic changes in tissues due to acute toxoplasmosis and will defiantly help the researchers in the better understanding of disease particularly in humans and putting up of suitable treatment regime for human subjects infected with acute toxoplasmosis.

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Acknowledgments

The authors are thankful to the Director, IVRI for providing the facilities and to the ICAR for the fellowship awarded to the first author during the perusal of his master’s programme.

Declaration

The experiments to the laboratory animal were done as per the approval of University Ethical Committee.

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Author notes

  1. Vikrant Sudan

    Present address: Department of Veterinary Parasitology, College of Veterinary Sciences & Animal Husbandry, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, India

Authors and Affiliations

  1. Indian Veterinary Research Institute (IVRI), Izatnagar, India

    Vikrant Sudan & Harkirat Singh

  2. Department of Parasitology, IVRI, Izatnagar, India

    A. K. Tewari

  3. Department of Parasitology, GADVASU, Ludhiana, India

    Harkirat Singh

  4. CADRAD, IVRI, Izatnagar, India

    R. Singh

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  1. Vikrant Sudan
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  2. A. K. Tewari
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  3. Harkirat Singh
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  4. R. Singh
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Correspondence to Vikrant Sudan.

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Sudan, V., Tewari, A.K., Singh, H. et al. Pathobiology of human RH strain induced experimental toxoplasmosis in murine model. J Parasit Dis 40, 840–844 (2016). https://doi.org/10.1007/s12639-014-0589-1

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  • DOI: https://doi.org/10.1007/s12639-014-0589-1

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