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A simple, efficient and inexpensive method for malaria parasites’ DNA catching from fixed Giemsa-stained blood slides

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Abstract

As parasitological or microscopic method is the gold standard and the best method for diagnosis of malaria, so fixed Geimsa-stained blood slides in the form of thick and thin blood smears are the most important data collections of malaria, especially historical slides. The parasites are dead but their DNA is valuable for many molecular biologic researches. A simple and efficient method for catching and extraction malaria parasites' DNA with a desired yield from dried and stained blood on slides is the first and major step. Introduction of an applicable, efficient and inexpensive DNA catching method and assessment of its performance in following molecular applications  was the main objective of present study.

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Acknowledgments

Our appreciation and thanks to Mr Masoud Yarian, head of Minab malaria lab for supporting samples collection, Isfahan University of Medical Sciences and Islamic Azad University of Falavarjan Branch for some support of this study.

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Authors and Affiliations

  1. Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran

    Abbas Ali Eskandarian

  2. Departments of Microbiology, Faculty of Biological Sciences, Islamic Azad University, Falavarjan Branch, Isfahan, Iran

    Sara Moradi & Saeed Abedi

Authors
  1. Abbas Ali Eskandarian
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  2. Sara Moradi
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  3. Saeed Abedi
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Correspondence to Abbas Ali Eskandarian.

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Eskandarian, A.A., Moradi, S. & Abedi, S. A simple, efficient and inexpensive method for malaria parasites’ DNA catching from fixed Giemsa-stained blood slides. J Parasit Dis 40, 833–837 (2016). https://doi.org/10.1007/s12639-014-0587-3

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  • DOI: https://doi.org/10.1007/s12639-014-0587-3

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