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Treating the Onset of Diabetes Using Probiotics Along with Prebiotic from Pachyrhizus erosus in High-Fat Diet Fed Drosophila melanogaster

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Abstract

The increasing mortality due to hypertension and hypercholesterolemia is directly linked with type-2 diabetes. This shows the lethality of the disease. Reports suggest that the prebiotics along with probiotics help in lowering the effects of type-2 diabetes. Prebiotic like inulin is best known for its anti-diabetic effect. The current study utilizes jicama extract as prebiotic source of inulin along with the bacterial strains with probiotic properties (Lactiplantibacillus plantarum and Enterococcus faecium) for treating type-2 diabetes in high-fat diet-induced Drosophila melanogaster model. The high-fat diet-induced Drosophila showed deposition of lipid droplets and formation of micronuclei in the gut. The larva and adult treated with probiotics and synbiotic (probiotic + prebiotic- inulin) comparatively reduced the lipid deposition and micronuclei number in the gut. The increased amount of triglyceride in the whole body of the fatty larva and adult indicated the onset of diabetes. The overexpression of insulin-like genes (Dilp 2) and (Dilp 5) confirmed the insulin resistance, whereas the expression was reduced in the larva and adult supplemented with probiotics and synbiotic. The reactive oxygen species level was reduced with the supplementation of probiotics. The weight, larva size, crawling speed and climbing were also altered in high-fat diet-induced Drosophila melanogaster. The study confirmed the effects of probiotics and synbiotic in successfully lowering diabetes in Drosophila. The study also proved the anti-diabetic potential of the probiotics. Further, it was also confirmed that the probiotics work better in the presence of prebiotic.

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Acknowledgements

The authors are thankful to the Science & Engineering Research Board (SERB) for financial support and National Institute of Technology (NIT), Rourkela, for providing the research facilities. The study was also supported by funding from Department of Biotechnology (DBT) under grant no. BT/PR21857/NNT/28/1238/2017 and SERB under grant no. EMR/2017/003054, received by Dr. Monalisa Mishra. Dr. Moumita Sahoo is highly acknowledged for providing the isolated probiotic strains for carrying out the research work.

Funding

AB is thankful to the Science & Engineering Research Board (SERB), (File No. IMP/2018/002120/EC), Government of India, for the financial support. NN is thankful to DST, INSPIRE for the financial support and SM is thankful to the Ministry of Human Resource and Development (MHRD). The work was also supported by funding from Department of Biotechnology (DBT) under grant no. BT/PR21857/NNT/28/1238/2017 and SERB under grant no. EMR/2017/003054, received by Dr. Monalisa Mishra.

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Authors and Affiliations

  1. Neural Developmental Biology Lab, Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769008, India

    Amrita Bhanja, Nibedita Nayak, Sumit Mukherjee & Monalisa Mishra

  2. Department of Food Process Engineering, National Institute of Technology, Rourkela, Odisha, 769008, India

    Parag Prakash Sutar

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  1. Amrita Bhanja
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  2. Nibedita Nayak
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  3. Sumit Mukherjee
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  5. Monalisa Mishra
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Contributions

Amrita Bhanja was involved in methodology, data curation, formal analysis, investigation, manuscript writing; Nibedita Nayak helped in data curation, investigation, formal analysis; Sumit Mukherjee contributed to data curation, investigation, formal analysis; Parag Prakash Sutar was involved in conceptualization, methodology, writing—original draft, writing—review and editing, funding acquisition; Monalisa Mishra helped in conceptualization, methodology, writing—original draft, writing—review and editing, funding acquisition, resources, supervision.

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Correspondence to Monalisa Mishra.

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Bhanja, A., Nayak, N., Mukherjee, S. et al. Treating the Onset of Diabetes Using Probiotics Along with Prebiotic from Pachyrhizus erosus in High-Fat Diet Fed Drosophila melanogaster. Probiotics & Antimicro. Prot. 14, 884–903 (2022). https://doi.org/10.1007/s12602-022-09962-0

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