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Characterization of Functional, Safety, and Probiotic Properties of Enterococcus faecalis AG5 Isolated From Wistar Rat, Demonstrating Adherence to HCT 116 Cells and Gastrointestinal Survivability

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Abstract

Gut microbiota remains a prominent source for a diverse range of potential probiotics. In this context, the current study explored the rectal region of experimental Wistar rat for the isolation of potent probiotic. Sixteen lactic acid bacteria (LAB), from rectal swab of Wistar rats, were subjected to evaluation of probiotic properties. Among all, AG5 was found unique with consistent probiotic properties and was further identified as Enterococcus faecalis AG5 (NCBI accession number KT248537) using 16S rDNA sequencing, followed by BLAST analysis. Since the Enterococci strains inhibit various food-borne pathogens efficiently while proving itself as a safe probiotic candidate, the study further evaluated the safety of the strain AG5 using primer specific PCR amplification which revealed the existence of gene encoding gelE, asa1, efaA, ace, vanA, and vanB and negative for cylA, hyl, and esp respectively. SEM analysis confirmed the adherence ability of AG5 to HCT 116 cells. Adherence was found to be non-colonial and scattered manner. Furthermore, the strain demonstrated a significant survivability during simulated gastrointestinal transit. Taken together, the E. faecalis AG5 was found potential probiotic candidate with future implication in both food and health industry.

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Acknowledgments

The authors would like to acknowledge Prof. Anu Baisel, Senior Assistant Professor, Department of English, School of Social Sciences and Languages, VIT University for English and grammar correction.

Funding

We would like to express gratitude to VIT University, Vellore, for financial support (RGEMS-2016).

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Authors and Affiliations

  1. Department of Integrative Biology, School of BioSciences and Technology, VIT University, Vellore, Tamil Nadu, 632014, India

    Alok Kumar Mishra & Asit Ranjan Ghosh

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Correspondence to Asit Ranjan Ghosh.

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Mishra, A.K., Ghosh, A.R. Characterization of Functional, Safety, and Probiotic Properties of Enterococcus faecalis AG5 Isolated From Wistar Rat, Demonstrating Adherence to HCT 116 Cells and Gastrointestinal Survivability. Probiotics & Antimicro. Prot. 10, 435–445 (2018). https://doi.org/10.1007/s12602-018-9387-x

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