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Utilization of Industrial Waste for the Production of Bio-Preservative from Bacillus licheniformis Me1 and Its Application in Milk and Milk-Based Food Products

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Abstract

The bio-preservative efficacy of a partially purified antibacterial peptide (ppABP) produced by Bacillus licheniformis Me1 in an economical medium developed using agro-industry waste was evaluated by direct application in milk and milk-based food products. The addition of ppABP in milk samples stored at 4 ± 2 °C and 28 ± 2 °C resulted in the growth inhibition of pathogens Listeria monocytogenes Scott A, Micrococcus luteus ATCC 9341, and Staphylococcus aureus FRI 722. The shelf life of milk samples with added ppABP increased to 4 days at 28 ± 2 °C, whereas curdling and off-odor were noticed in samples without ppABP. Furthermore, the milk samples with ppABP were sensorily acceptable. Antilisterial effect was also observed in cheese and paneer samples treated with ppABP. These results clearly indicate that the ppABP of B. licheniformis Me1 can be utilized as a bio-preservative to control the growth of spoilage and pathogenic bacteria, thereby reducing the risk of food-borne diseases.

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Acknowledgements

The authors wish to acknowledge the Director of CSIR-CFTRI, Mysore, for providing the facilities. N.V. acknowledges CSIR, New Delhi, for the fellowship. This work was carried out in the institute funded project, MLP 083 (PMC comm No. RS/121/2012/215).

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Authors and Affiliations

  1. Microbiology and Fermentation Technology Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020, India

    Vadakedath Nithya & Prakash M. Halami

  2. Food Packaging Technology Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020, India

    Maya Prakash

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  1. Vadakedath Nithya
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  2. Maya Prakash
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  3. Prakash M. Halami
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Correspondence to Prakash M. Halami.

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Nithya, V., Prakash, M. & Halami, P.M. Utilization of Industrial Waste for the Production of Bio-Preservative from Bacillus licheniformis Me1 and Its Application in Milk and Milk-Based Food Products. Probiotics & Antimicro. Prot. 10, 228–235 (2018). https://doi.org/10.1007/s12602-017-9319-1

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  • DOI: https://doi.org/10.1007/s12602-017-9319-1

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