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Protective Effects of Mekabu Aqueous Solution Fermented by Lactobacillus plantarum Sanriku-SU7 on Human Enterocyte-Like HT-29-luc Cells and DSS-Induced Murine IBD Model

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Abstract

Most wakame Undaria pinnatifida, a brown algae, products are made from the frond portion. In this study, the polysaccharide content and antioxidant property of aqueous extract solutions (AESs) of the four parts (frond: wakame, stem of the frond: kuki-wakame, sporophyll: mekabu, and kuki-mekabu) of wakame were investigated. Polysaccharide content was high in both the wakame and mekabu. Superoxide anion (O2 −) radical-scavenging capacities were high in the mekabu. These AESs could be fermented by Lactobacillus plantarum Sanriku-SU7. The O2 − radical-scavenging activity of the kuki-wakame, mekabu, and kuki-mekabu were increased by the fermentation. Fermented mekabu clearly showed a protective effect on human enterocyte-like HT-29-luc cells and in a mouse model of dextran sodium sulphate-induced inflammatory bowel disease (IBD). These results suggest that the mekabu fermented by L. plantarum Sanriku-SU7 has anti-IBD effect related to O2 − radical-scavenging.

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Acknowledgments

This work was supported by the SANRIKU Fisheries Research and Education Project (Iwate University, Ministry of Education, Culture, Sports, Science and Education and Technology [MEXT]) in Japan, The Towa Foundation for Food Science and Research, Tokyo, Japan, and TERRADA Warehouse, Tokyo, Japan.

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Authors and Affiliations

  1. Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-City, Tokyo, 108-8477, Japan

    Maki Nemoto, Takashi Kuda, Mika Eda, Hajime Takahashi & Bon Kimura

  2. Office of Liaison and Cooperative Research, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-City, Tokyo, 108-8477, Japan

    Hiroshi Yamakawa

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  1. Maki Nemoto
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  2. Takashi Kuda
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Correspondence to Takashi Kuda.

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Maki Nemoto, Takashi Kuda, Mika Eda, Hiroshi Yamakawa, Hajime Takahashi, and Bon Kimura declare that they have no conflict of interest.

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Nemoto, M., Kuda, T., Eda, M. et al. Protective Effects of Mekabu Aqueous Solution Fermented by Lactobacillus plantarum Sanriku-SU7 on Human Enterocyte-Like HT-29-luc Cells and DSS-Induced Murine IBD Model. Probiotics & Antimicro. Prot. 9, 48–55 (2017). https://doi.org/10.1007/s12602-016-9226-x

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