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Immunomodulation of Monocytes by Probiotic and Selected Lactic Acid Bacteria

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Abstract

Some lactic acid bacteria (LAB), especially bacteria belonging to the genus Lactobacillus, are recognized as common inhabitants of the human gastrointestinal tract and have received considerable attention in the last decades due to their postulated health-promoting effects. LAB and probiotic bacteria can modulate the host immune response. However, much is unknown about the mediators and mechanisms responsible for their immunological effect. Here, we present a study using cytokine secretion from the monocytic cell line THP-1 and NF-κB activation in the monocytic cell line U937-3xkB-LUC to elucidate immune stimulating abilities of LAB in vitro. In this study, we investigate both commercially available and potential probiotic LAB strains, and the role of putative surface proteins of L. reuteri using mutants. L. reuteri strains induced the highest cytokine secretion and the highest NF-κB activation, whereas L. plantarum strains and L. rhamnosus GG were low inducers/activators. One of the putative L. reuteri surface proteins, Hmpref0536_10802, appeared to be of importance for the stimulation of THP-1 cells and the activation of NF-κB in U937-3xkB-LUC cells. Live and UV-inactivated preparations resulted in different responses for two of the strains investigated. Our results add to the complexity in the interaction between LAB and human cells and suggest the possible involvement of secreted pro- and anti-inflammatory mediators of LAB. It is likely that it is the sum of bacterial surface proteins and bacterial metabolites and/or secreted proteins that induce cytokine secretion in THP-1 cells and activate NF-κB in U937-3xkB-LUC cells in this study.

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Acknowledgments

This work was supported by the Fund for the Research Levy on Agricultural Products. The authors thank Marte Taraldrud for excellent technical assistance. The U937-3xkB-LUC cell line was a kind gift from Rune Blomhoff, University of Oslo.

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The authors’ declares that they have no conflict of interest.

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Authors and Affiliations

  1. Nofima, Norwegian Institute of Food, Fisheries, and Aquaculture Research, P.O. Box 210, 1431, Ås, Norway

    Hanne Jensen, Signe Marie Drømtorp, Lars Axelsson & Stine Grimmer

  2. Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O. Box 5003, 1432, Ås, Norway

    Hanne Jensen

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  1. Hanne Jensen
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Correspondence to Hanne Jensen.

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Jensen, H., Drømtorp, S.M., Axelsson, L. et al. Immunomodulation of Monocytes by Probiotic and Selected Lactic Acid Bacteria. Probiotics & Antimicro. Prot. 7, 14–23 (2015). https://doi.org/10.1007/s12602-014-9174-2

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