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Morphological and biochemical basis of resistance in okra to cotton jassid, Amrasca biguttula biguttula (Ishida)

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Fifteen okra germplasm entries viz. accessions: IC0506027, IC0506118 and EC0306728; Abelmoschus spp.: Abelmoschus tuberculatus, Abelmoschus moschatus, Abelmoschus angulosus, Abelmoschus tetraphyllus, Abelmoschus manihot and Abelmoschus caillei; genotypes: POL-6 and POL-7; and four cultivated varieties: Punjab 8, Punjab Padmini, Punjab 7 and Pusa Sawani were screened against jassid, Amrasca biguttula biguttula (Ishida) in field at Vegetable Research Farm, Department of Vegetable Science, Punjab Agricultural University, Ludhiana, India during Kharif 2015. Different morphological and biochemical parameters of leaves of the selected entries were also studied. The correlation between jassid nymphal population and mid vein hair density, total phenols and tannins was negative and significant (r = −0.67, −0.83, −0.75, respectively); negative and non-significant for hair length, angle of insertion of hair, total sugars and silica (r = −0.40, −0.49, −0.63 and −0.59, respectively) and positive and highly significant for lacination index, reducing sugars and lignins (r = 0.82, 0.95 and 0.90, respectively). Abelmoschus spp. Abelmoschus tetraphyllus, Abelmoschus angulosus and Abelmoschus moschatus were found to be field resistant on the basis of significantly lower pooled jassid nymphal population (1.56–1.99), jassid injury index (1.16–1.27) and susceptibility index (2.70–2.92). High degree of resistance in Abelmoschus tetraphyllus, Abelmoschus angulosus and Abelmoschus moschatus was found to be associated with high hair density (4.75–7.50), longer hair (1285.00–1513.20 μm), more erect hair (83.40–95.20°), broad leaves, high total sugars (15.21–18.36 mg/g), total phenols (1.52–1.58 mg/g), tannins (26.12–31.48 mg/g) and silica (32.66–33.17 mg/g) and low levels of reducing sugars (2.50–3.39 mg/g). Abelmoschus tuberculatus, A. manihot, IC0506027 and EC0306728 were found moderately field resistant with variable levels of morphological and biochemical parameters. High hair density, broad leaves, moderate levels of total sugars, reducing sugars, total phenols, tannins and silica seems to be associated with moderate levels of resistance in these entries. The variable levels of above mentioned parameters in moderately resistant entries also indicate that a single factor is not responsible for resistance but combination of different factors may be conferring resistance to jassid.

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Acknowledgements

We are thankful to Dr. Anu Kalia, Assistant Professor (Nanoscience), PAU, Ludhiana for her guidance in taking SEM micrographs in her lab and also to Dr. Vijay Kumar, Senior Entomologist, and Dr. K S Suri, Senior Entomologist, Department of Entomology, PAU, Ludhiana for their critical discussions and suggestions.

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Authors and Affiliations

  1. Department of Entomology, Punjab Agricultural University, Ludhiana, India

    Ramandeep Kaur Sandhi

  2. Department of Vegetable Science, Punjab Agricultural University, Ludhiana, India

    Sandeep Kaur Sidhu, Abhishek Sharma, Neena Chawla & Mamta Pathak

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  1. Ramandeep Kaur Sandhi
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  2. Sandeep Kaur Sidhu
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Correspondence to Ramandeep Kaur Sandhi.

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Sandhi, R.K., Sidhu, S.K., Sharma, A. et al. Morphological and biochemical basis of resistance in okra to cotton jassid, Amrasca biguttula biguttula (Ishida). Phytoparasitica 45, 381–394 (2017). https://doi.org/10.1007/s12600-017-0589-7

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