Skip to main content
SpringerLink
Log in

Association of phytic acid content with biotic stress tolerance in mungbean (Vigna radiata L. Wilczek)

  • Published:
Phytoparasitica Aims and scope Submit manuscript

Abstract

Phytic acid (PA), though an antinutritional factor that causes mineral deficiency in monogastric animals but also reported to be associated with expression of stress tolerance genes. The present study was conducted to find out association of seed PA with tolerance to yellow mosaic disease (YMD), powdery mildew disease (PMD) and storage pest bruchid in mungbean. Ninety four genotypes were screened for YMD by using infector rows technique in summer 2011 and 2012 and PMD screening was done in rabi 2012 in the field conditions and using excised leaf technique in growth chamber. Screening for bruchid (Callosobruchus chinensis L.) was carried out by following standard procedure. PA and inorganic phosphorous (IP) were estimated from seeds by colorimetric method and Chen’s modified method respectively. Among 94 genotypes, 47 were found susceptible while out of 47 resistant genotypes, 37, 9 and 1 were found resistant to YMD, PMD and bruchid respectively. PA was found to be highest in bruchid resistant genotype followed by PMD and YMD. Along with the resistance genes, high seed PA (>8 mgg-1) was required for resistant reaction to YMD and PMD, while for bruchid resistance very high PA (~18 mgg-1) should be present in seeds. If the concentration of PA is reduced below this level my leads to decrease in tolerance to biotic stresses, even though the resistance gene is present in the plant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anonymous. (2015). Project coordinator’s report (mungbean and urdbean) 2014-15. All India coordinated research project on MULLaRP. Kanpur, India: Indian Institute of Pulses Research.

    Google Scholar 

  • Bregitzer, P., & Raboy, V. (2006). Effects of four independent low phytate mutations on barley agronomic performance. Crop Science, 46, 1318–1322.

    Article  Google Scholar 

  • Brown, K. H., & Solomons, N. W. (1991). Nutritional problems of developing countries. Infectious Disease Clinics of North America, 5, 297–317.

    CAS  PubMed  Google Scholar 

  • Chen, P. S., Toribara, T. Y., & Warner, H. (1956). Micro determination of phosphorus. Analytical Chemistry, 28, 1756–1758.

    Article  CAS  Google Scholar 

  • Dahiya, P. K., Linnemann, A. R., Nout, M. J. R., Boekel, M. A. J. S., & Grewal, R. B. (2013). Nutrient composition of selected newly bred and established mungbean varieties. LWT - Food Science and Technology, 54, 249–256.

    Article  CAS  Google Scholar 

  • Debnath, D., Sahu, N. P., Pal, A. K., Baruah, K., Yengkokpam, S., & Mukherjee, S. C. (2005). Present scenario and future prospects of phytase in aquafeed -review. Asian-Australasian Journal of Animal Sciences, 18, 1800–1812.

    Article  CAS  Google Scholar 

  • Dhole, V. J., & Reddy, K. S. (2013). Development of a SCAR marker linked with a MYMV resistance gene in mungbean (Vigna radiata L. Wilczek). Plant Breeding, 132, 127–132.

    Article  CAS  Google Scholar 

  • Dhole, V. J., & Reddy, K. S. (2015). Genetic variation for phytic acid content in mungbean (Vigna radiata L. Wilczek). The crop Journal, 3, 157–162.

    Article  Google Scholar 

  • Dongre, T. K., Pawar, S. E., Thakare, R. G., & Harwalkar, M. R. (1996). Identification of resistant source to cowpea weevil [Callosobruchus maculatus (F.)] in Vigna sp. and inheritance of their resistance in black gram (Vigna mungo var. mungo). Journal of Stored Products Research, 32, 201–204.

    Article  Google Scholar 

  • Ertl, D. S., Young, K. A., & Raboy, V. (1998). Plant genetic approaches to phosphorus management in agricultural production. Journal of Environmental Quality, 27, 299–304.

    Article  CAS  Google Scholar 

  • Gao, Y., Shang, C., Saghai Maroof, M. A., Biyashev, R. M., Grabau, E. A., Kwanyuen, P., Burton, J. W., & Buss, G. R. (2007). A modified colorimetric method for phytic acid analysis in soybean. Crop Science, 47, 1797–1803.

    Article  CAS  Google Scholar 

  • Green, E. S., Zangerl, A. R., & Berenbaum, M. R. (2001). Effect of phytic acid and xanthotoxin on growth and detoxification in caterpillar. Journal of Chemical Ecology, 27, 1763–1773.

    Article  CAS  PubMed  Google Scholar 

  • Hande, P., Mondal, S., Badigannavar, A. M., & D’Souza, S. F. (2013). Genetic variability of phytic acid- phosphorus and inorganic phosphorus in cultivated groundnut (Arachis hypogaea L.). Plant Genetic Resources: Characterization and utilization, 1-6.

  • Khattak, G. S. S., Haq, M. A., Rana, S. A., Abass, G., & Irfag, M. (2000). Effect of mungbean yellow mosaic virus (MYMV) on yields and yield components of mungbean (Vigna radiata L. Wilczek). Kasetsart Journal (Natural Sciences), 34, 12–16.

    Google Scholar 

  • Latta, M., & Eskin, M. A. (1980). Simple and rapid colorimetric method for phytate determination. Journal of Agricultural and Food Chemistry, 28, 1315–1317.

    Article  Google Scholar 

  • Marzo, F., Andres, A., Maria, V. C., & Ruben, A. (1997). Fertilization effects of phosphorus and sulfur on chemical composition of seeds of Pisum sativum L. and relative infestation by Bruchus pisorum L. Journal of Agricultural and Food Chemistry, 45, 1829–1833.

    Article  CAS  Google Scholar 

  • Murphy, A. M., Otto, B., Brearley, C. A., Carr, J. P., & Hanke, D. E. (2008). A role for inositol hexakisphosphate in the maintenance of basal resistance to plant pathogens. The Plant Journal, 56, 638–652.

    Article  CAS  PubMed  Google Scholar 

  • Reddy, K. S., Pawar, S. E., & Bhatia, C. R. (1987). Screening for powdery mildew (Erysiphe polygoni DC) resistance in mungbean (Vigna radiata L. Wilczek) using excised leaves. Proceedings of Indian Academy of Science (Plant Science), 97, 365–369.

    Google Scholar 

  • Reddy, K. S., Pawar, S. E., & Bhatia, C. R. (1994). Inheritance of powdery mildew (Erysiphe polygoni DC) resistance in mungbean (Vigna radiata L. Wilczek). Theoretical and Applied Genetics, 88, 945–948.

    Article  CAS  PubMed  Google Scholar 

  • Singh, M., & Krikorian, A. D. (1982). Inhibition of Trypsin Activity in Vitro by Phytate. Journal of Agricultural and Food Chemistry, 30, 799–800.

    Article  CAS  Google Scholar 

  • Sompong, U., Kaewprasit, C., Nakasathien, S., & Srinives, P. (2010). Inheritance of seed phytate in mungbean (Vigna radiata L. Wilczek). Euphytica, 171, 389–396.

    Article  CAS  Google Scholar 

  • Srinivasan, T., & Durairaj, C. (2007). Biochemical basis of resistance in rice bean, Vigna umbellata Thunb. (Ohwi and Ohashi) against Callosobruchus maculatus F. Journal of Entomology, 4, 371–378.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to Dr. S. P. Kale, Head, NABTD, BARC, Mumbai, for his guidance and support throughout this research work. We are especially thankful to Mr. S. M. Bhatkar and P. N. Thokal for the help in conducting the field experiments.

Author information

Authors and Affiliations

  1. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India

    Vinod Janardan Dhole & Kandali Srinivasulu Reddy

Authors
  1. Vinod Janardan Dhole
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kandali Srinivasulu Reddy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vinod Janardan Dhole.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Fig S1

(JPG 1713 kb)

Fig S2

(JPG 1803 kb)

Fig S3

(JPG 261 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dhole, V.J., Reddy, K.S. Association of phytic acid content with biotic stress tolerance in mungbean (Vigna radiata L. Wilczek). Phytoparasitica 44, 261–267 (2016). https://doi.org/10.1007/s12600-016-0514-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12600-016-0514-5

Keywords

Search

Navigation