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In vivo evaluation of essential oils and biocontrol agents combined with heat treatments on basil cv Genovese Gigante seeds against Fusarium oxysporum f. sp. basilici

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Abstract

Seed treatments with essential oils (from savory and thyme) and biocontrol agents (Pseudomonas spp. and Fusarium oxysporum) have been evaluated in vivo after dry hot air treatments against Fusarium oxysporum f. sp. basilici on basil seeds. The savory and thyme essential oils showed a significant pathogen control activity because of their innate antifungal activity and because of the seed application method, but the dry hot pre-treatment did not show any obvious effect on the performance of the essential oil treatments. The dry heat treatment improved the Pseudomonas seed dressing effect against F.oxysporum f. sp. basilici, and showed important reductions in plant infection and the disease index on the treated seed plants, without any negative effect on seed germination. However, the pathogen control provided by the heat treatments combined with the application of the biocontrol agents never reached the same performance as the chemical treatments considered as the reference. Thus, short dry heat treatments on basil seeds have been shown to be a valid but complementary seed disinfection method against Fusarium wilt.

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Acknowledgements

The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007–2013) under Grant Agreement No 311875 "Seed health: development of seed treatment methods, evidence for seed transmission and assessment of seed health" (TESTA). The authors thank Dr. Marguerite Jones for language revision.

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Authors and Affiliations

  1. Centre of Competence for the Innovation in the Agro-Environmental Sector (AGROINNOVA), University of Torino, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy

    Jorge Giovanny Lopez-Reyes, Giovanna Gilardi, Angelo Garibaldi & Maria Lodovica Gullino

  2. Department of Agricultural, Forest and Food Sciences and Technologies (DI.S.A.F.A.), University of Torino, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy

    Maria Lodovica Gullino

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  1. Jorge Giovanny Lopez-Reyes
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  2. Giovanna Gilardi
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  4. Maria Lodovica Gullino
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Correspondence to Jorge Giovanny Lopez-Reyes.

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Lopez-Reyes, J.G., Gilardi, G., Garibaldi, A. et al. In vivo evaluation of essential oils and biocontrol agents combined with heat treatments on basil cv Genovese Gigante seeds against Fusarium oxysporum f. sp. basilici . Phytoparasitica 44, 35–45 (2016). https://doi.org/10.1007/s12600-016-0504-7

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