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Effects of streptozotocin-induced diabetes on leg muscle contractile properties and motor neuron morphology in rats

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Abstract

Skeletal muscle fiber subtypes are differentially sensitive to diabetes-related pathology; For example, fast-twitch muscles exhibit severe decreases in contraction force while slow-twitch muscles demonstrate prolonged half-relaxation time. However, such alterations have only been examined after a relatively short period following diabetes onset, with no information available regarding muscle damage caused by longer disease periods (>20 weeks). This study examined alterations in the contractile properties of the medial gastrocnemius (fast-twitch) and soleus (slow-twitch) muscles, as well as morphological changes in their motor neurons 12 and 22 weeks after diabetes onset. Adult male Wistar rats were divided into diabetic (12- or 22-week post-streptozotocin injection) and age-matched control groups. Electrically evoked maximum twitch and tetanic tension were recorded from leg muscles. Additionally, motor neuron number and cell body size were examined. At 12 weeks after diabetes onset, decreases in twitch force were observed predominantly in medial gastrocnemius muscles, while soleus muscles exhibited prolonged half-relaxation time. However, these differences became ambiguous at 22 weeks, with decreased twitch force and prolonged half-relaxation time observed in both muscles. On the other hand, reduction in soleus motor neurons was observed 12 weeks after diabetes onset, while medial gastrocnemius motor neurons were diminished at 22 weeks. These data indicate that experimental diabetes induces differential damage to medial gastrocnemius and soleus muscles as well as motor neurons. These diabetes-induced differences may partly underlie the differential deficits observed in gastrocnemius and soleus.

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Acknowledgments

We gratefully acknowledge the work of members of our laboratory.

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Authors and Affiliations

  1. Department of Occupational Therapy, Graduate School of Kyorin University, 5-4-1 Simorenzyaku, Mitaka-city, Tokyo, 181-8612, Japan

    Toru Tamaki, Naomi Oshiro & Masatoshi Niwa

  2. Department of Physical Therapy, Health Science University, 7187 Kodachi, Fujikawaguchiko-town, Yamanashi, 401-0380, Japan

    Toru Tamaki, Ken Muramatsu & Masako Ikutomo

  3. Department of Physical Therapy, Seijoh University, 2-172 Fukinodai, Tokai City, Aichi, 476-8588, Japan

    Hisae Hayashi

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  1. Toru Tamaki
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  2. Ken Muramatsu
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  3. Masako Ikutomo
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  4. Naomi Oshiro
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  5. Hisae Hayashi
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  6. Masatoshi Niwa
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Correspondence to Toru Tamaki.

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Tamaki, T., Muramatsu, K., Ikutomo, M. et al. Effects of streptozotocin-induced diabetes on leg muscle contractile properties and motor neuron morphology in rats. Anat Sci Int 93, 502–513 (2018). https://doi.org/10.1007/s12565-018-0444-z

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  • DOI: https://doi.org/10.1007/s12565-018-0444-z

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