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Histological and mucin histochemical study of the small intestine of the Persian squirrel (Sciurus anomalus)

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Abstract

This article describes the histological and mucin histochemical properties of the small intestine of the Persian squirrel (Sciurus anomalus). This species is widely distributed in the Middle East and can be found as a companion animal. The histological studies revealed that the plicae circulares were not visible in the tunica mucosa. The maximum height and width of the villi were observed in the duodenum, which then decreased toward the ileum. The muscularis mucosa was scattered, whereas the tunica submucosa was composed of dense connective tissue. The lymphatic nodules were seen in the submucosa of the distal part of the jejunum and ileum, and Brunner’s glands were embedded in the initial portion of the duodenum. The tunica muscularis was significantly thicker in the ileum, and the circular muscle layer was thicker than the longitudinal muscle layer throughout the entire length of the small intestine. The mucin histochemistry, which was examined using the periodic acid-Schiff (PAS) and alcian blue (AB) (pH 1.0 and 2.5) and also PAS–AB (pH 2.5) and aldehyde fuchsin-AB (pH 2.5) techniques coupled with methylation and saponification reaction for some sections, showed that the small intestine mucous content included both carboxylated and sulfated acidic mucins with few neutral mucins. The results of this study contribute to the knowledge of the histological and histochemical characteristics of the gastrointestinal tracts of exotic mammals and provide data for comparison with other mammals.

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References

  • Ahnen DJ, Poulsom R, Stamp GWH, Elia G, Pike C, Jeffery R, Longcroft J, Rio MC, Chambon P, Wright NA (1994) The ulceration-associated cell lineage (UACL) reiterates the Brunner’s glands differentiation programme but acquires the proliferative organisation of the gastric glands. J Pathol 173:317–326

    Article  PubMed  CAS  Google Scholar 

  • Bancroft JD, Cook HC (1994) Manual of histological techniques and their diagnostic application. Churchill Livingstone, London

    Google Scholar 

  • Banks WJ (1993) Applied veterinary histology. Mosby-Year book, London

    Google Scholar 

  • Bonucci E (1981) Manuale di Istochimica. Lombardo Editore, Roma

    Google Scholar 

  • Carey HV, Sills NS (1962) Hibernation enhances d-glucose uptake by intestinal brush border membrane vesicles in ground squirrels. J Comp Physiol 166:254–261

    Google Scholar 

  • Cochrane W, Davies DV, Palfrey AJ, Stockwell RA (1964) The histochemistry and electronmicroscopy of Brunner’s glands in the guinea-pig. J Anat 98:1–10

    PubMed  CAS  Google Scholar 

  • Dellman HD (1993) Textbook of veterinary histology. Lea & Febiger, Philadelphia

    Google Scholar 

  • Forman GL (1971) Histochemical differences in gastric mucus of bats. J Mammal 52:191–193

    Article  PubMed  CAS  Google Scholar 

  • Hosoyamada Y, Sakai T (2005) Structural and mechanical architecture of the intestinal villi and crypts in the rat intestine: integrative reevaluation from ultrastructural analysis. Anat Embryol 210:1–12

    Article  PubMed  Google Scholar 

  • Inokuchi H, Kawai K, Takeuchi Y, Sano Y (1982) Immunohistochemical demonstration of EC cells in rat gastrointestinal tract. Histochemistry 27:453–456

    Article  Google Scholar 

  • Khazraiinia P, Rostami A, Haddadzadeh HR, Nassiri SM (2008) Hematological characteristics and hemoglobin typing of the Persian Squirrel (Sciurus anomalus). J Exot Pet Med 17:44–48

    Article  Google Scholar 

  • Krause WJ (1981) Morphological and histochemical observations on the duodenal glands of eight wild ungulate species native to North America. Am J Anat 162:167–181

    Article  PubMed  CAS  Google Scholar 

  • Krause WJ (2000) Brunner’s glands: a structural, histochemical and pathological profile. Prog Histochem Cytochem 35:255–367

    Article  CAS  Google Scholar 

  • Ku SK, Lee HS, Lee JH (2006) The regional distribution and relative frequency of gastrointestinal endocrine cells in the nude mice, Balb/c-nu/nu: an immunohistochemical study. Anat Histol Embryol 35:104–110

    Article  PubMed  CAS  Google Scholar 

  • Kurtz CC, Care HV (2007) Seasonal changes in the intestinal immune system of hibernating ground squirrels. Dev Comp Immunol 31:415–428

    Article  PubMed  CAS  Google Scholar 

  • Lee HS, Hashimoto Y, Kon Y, Sugimura M (1991) An immunohistochemical study of the gastro-entero-pancreatic endocrine cells in the alimentary tract of the Korean Tree Squirrel, Sciurus vulgaris corea. Jpn J Vet Res 39:117–131

    PubMed  CAS  Google Scholar 

  • Mowry RW (1956) Observations on the use of sulphuric ether for the sulphation of hydroxyl groups in tissue sections. J Histochem Cytochem 4:407

    Google Scholar 

  • Obuoforibo AA (1975) Mucosubstances in Brunner’s glands of the mouse. J Anat 119:287–297

    PubMed  CAS  Google Scholar 

  • Oduor-Okelo D (1976) Histochemistry of the duodenal glands of the cat and horse. Acta Anat 94:449–456

    Article  PubMed  CAS  Google Scholar 

  • Ota H, Nakayama J, Momose M, Kurihara M, Ishihara K, Hotta K, Katsuyama T (1998) New monoclonal antibodies against gastric gland mucous cell-type mucins: a comparative immunohistochemical study. Histochem Cell Biol 110:113–119

    Article  PubMed  CAS  Google Scholar 

  • Poddar S, Jacob S (1979) Mucosubstance histochemistry of Brunner’s glands, pyloric glands and duodenal goblet cells in the ferret. Histochemistry 65:67–81

    Article  PubMed  CAS  Google Scholar 

  • Sato A, Spicer SS (1980) Ultrastructural cytochemistry of complex carbohydrates of gastric epithelium in the guinea pig. Am J Anat 159:307–329

    Article  PubMed  CAS  Google Scholar 

  • Satoh Y, Yamano M, Matsudo M, Ono K (1990) Ultrastructure of Paneth cells in the intestine of various mammals. J Electron Micro Tech 16:69–80

    Article  CAS  Google Scholar 

  • Schumacher U, Duku M, Katoh M, Jurns J, Krause WJ (2004) Histochemical similarities of mucins produced by Brunner’s glands and pyloric glands: a comparative study. Anat Rec A 278A:540–550

    Article  Google Scholar 

  • Speece AJ (1964) Histochemical distribution of lysozyme activity in organs of normal mice and radiation chimeras. J Histochem Cytochem 15:225–242

    Google Scholar 

  • Spicer SS, Meyer DB (1960) Histochemical differentiation of acid mucopolysaccharides by means of combined aldehyde fuchsin–alcian blue staining. Am J Clin Pathol 33:453–460

    CAS  Google Scholar 

  • Takehana K, Abe M, Iwasa K, Hıraga T (1989) Histochemistry of complex carbohydrates in the horse duodenal gland. Nihon Juigaku Zasshi 51:909–916

    Article  PubMed  CAS  Google Scholar 

  • Takehana K, Abe M, Iwasa K, Hiraga T, Miyata H (1991) Carbohydrate histochemistry of bovine duodenal glands. J Vet Med Sci 53:699–706

    Article  PubMed  CAS  Google Scholar 

  • Takehana K, Masty J, Yamaguchi M, Kobayashi A, Yamada O, Kuroda M, Park YS, Iwasa K, Abe M (1998) Fine structural and histochemical study of equine Paneth cells. Anat Histol Embryol 27:125–129

    Article  PubMed  CAS  Google Scholar 

  • Thorington RW Jr, Darrow K (2000) Anatomy of the squirrel wrist: bones, ligaments, and muscles. J Morphol 246:85–102

    Article  PubMed  Google Scholar 

  • Treuting PM, Valasek MA, Dintzis SM (2012) Upper gastrointestinal tract. In: Treuting PM, Dintzis SM (eds) Comparative anatomy and histology—a mouse and human atlas. Elsevier, USA, pp 157–175

    Google Scholar 

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Acknowledgments

The authors are particularly grateful to Dr. A. Bahonar for his valuable assistance in the statistical analyses.

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Authors and Affiliations

  1. Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

    Zahra Tootian & Mohammad Taghi Sheibani

  2. Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

    Javad Sadeghinezhad

  3. Department of Anatomy, Tehran Medical Branch, Islamic Azad University, Tehran, Iran

    Simin Fazelipour

  4. Department of Veterinary Medical Science, University of Bologna, Ozzano dell’Emilia (Bologna), Italy

    Javad Sadeghinezhad, Nadia De Sordi & Roberto Chiocchetti

Authors
  1. Zahra Tootian
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  2. Javad Sadeghinezhad
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  3. Mohammad Taghi Sheibani
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  4. Simin Fazelipour
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  5. Nadia De Sordi
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  6. Roberto Chiocchetti
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Correspondence to Roberto Chiocchetti.

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Tootian, Z., Sadeghinezhad, J., Sheibani, M.T. et al. Histological and mucin histochemical study of the small intestine of the Persian squirrel (Sciurus anomalus). Anat Sci Int 88, 38–45 (2013). https://doi.org/10.1007/s12565-012-0159-5

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  • DOI: https://doi.org/10.1007/s12565-012-0159-5

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