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Amberjack Seriola dumerili interleukin-10 negatively suppresses host cell-mediated immunity

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  • Aquaculture
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Abstract

Interleukin-10 (IL-10) is an anti-inflammatory cytokine and negatively regulates cell-mediated immunity (CMI) induction by inhibiting cytokine production in type 1 T helper cells. IL-10 genes have been isolated from several fish, and inflammatory cytokine inhibition by IL-10 has been well examined. However, a CMI regulator of IL-10 in fish has not yet been identified. In this study, we cloned the IL-10 gene in amberjack Seriola dumerili and analyzed its function using its recombinant protein (rIL-10). In an in vitro culture experiment, gene expression of inflammatory cytokines was suppressed in leukocytes incubated with rIL-10 compared with cells that only received Nocardia seriolae stimulation. This result suggests amberjack IL-10 has conserved function as an inflammatory cytokine inhibitor. Bactericidal activity of amberjack cells against intracellular pathogen stimulation was decreased in a rIL-10 dose-dependent manner. Furthermore, a significant reduction in the T-bet/GATA-3 ratio was observed in N. seriolae living cell (LC) + rIL-10-injected fish. Taken together, these results suggest amberjack rIL-10 suppresses CMI induction both in vitro and in vivo. In addition, the number of IgM+ cells among spleen leukocytes in N. seriolae + rIL-10-injected fish was higher than in only N. seriolae LC, suggesting that Th2-dominant immunity was induced by adding rIL-10.

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Acknowledgements

We thank Mr. Soetsu Yanagi and Mr. Yoshiaki Imaoka (Kagoshima Prefectural Fisheries Technology and Development Center, Japan) for assistance with fish maintenance. This work was supported by JSPS KAKENHI (Grant Nos. 25292129 and 25450305).

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Authors and Affiliations

  1. The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan

    Megumi Matsumoto, Kyosuke Araki, Yutaka Takeuchi, Kazuhiro Shiozaki & Atsushi Yamamoto

  2. Faculty of Veterinary Medicine, Alexandria University, Edfina, 22758, Egypt

    Mahumoud Tanekey Amer

  3. Faculty of Fisheries, Kagoshima University, 4-50-20 Shimoarata, Kagoshima, 890-0056, Japan

    Kyosuke Araki, Kazuma Hayashi, Yutaka Takeuchi, Kazuhiro Shiozaki & Atsushi Yamamoto

  4. Division of Gene Research, Research Support Center, Kagoshima University, 1-21, Korimoto, Kagoshima, 890-0065, Japan

    Atsushi Nishitani

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  1. Megumi Matsumoto
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Correspondence to Atsushi Yamamoto.

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Matsumoto, M., Amer, M.T., Araki, K. et al. Amberjack Seriola dumerili interleukin-10 negatively suppresses host cell-mediated immunity. Fish Sci 84, 857–867 (2018). https://doi.org/10.1007/s12562-018-1223-4

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  • DOI: https://doi.org/10.1007/s12562-018-1223-4

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