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Gene expression profile analysis indicate SEPALLATA3 and AGL15 potentially involved in arabidopsis silique dehiscence by regulating glycosyl hydrolase

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Abstract

Fruit dehiscence is an essential developmental process for certain economic crops that dramatically impacts crop yields and involves the altered regulation of thousands of genes and corresponding biological processes. The regulation of fruit dehiscence is complex; although some regulatory genes have been characterized, the overall mechanism is still unknown. In this study, we used microarray analysis to screen the expression patterns of genes during three stages of Arabidopsis thaliana silique dehiscence (stages 11, 15, and 17). Differentially expressed genes (DEGs) were detected, and the combination of functional enrichment analysis with Short Time-series Expression Miner (STEM) clustering analysis was used to explore the overall gene expression changes and related functions during fruit dehiscence. A total of 9 specific gene expression clusters and corresponding functions were found. Moreover, transcription factor (TF) enrichment analysis for these gene clusters showed that two TFs (SEPALLATA3 and AGL15) maybe key regulatory factors for silique dehiscence because they target many glycosyl hydrolases in a certain cluster related to cell wall degradation. Taken together, our data enable connections to be drawn among specic biological functions, genes and TFs, which supports the development of network models to elucidate the process of silique dehiscence.

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Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou, 510631, China

    Xingshan Jiang & Xiaojing Wang

  2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China

    Hanjun He, Ting Wang & Hong Wu

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  1. Xingshan Jiang
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  2. Hanjun He
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  3. Ting Wang
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  4. Xiaojing Wang
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  5. Hong Wu
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Correspondence to Xiaojing Wang or Hong Wu.

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Jiang, X., He, H., Wang, T. et al. Gene expression profile analysis indicate SEPALLATA3 and AGL15 potentially involved in arabidopsis silique dehiscence by regulating glycosyl hydrolase. J. Plant Biol. 59, 133–142 (2016). https://doi.org/10.1007/s12374-016-0567-5

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