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Production of Humic Acid by a Bacillus megaterium Strain Using Vinasse

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Abstract

Humic acid can be produced by a Bacillus megaterium strain, AS019, using vinasse (sugarcane mill wastewater) at an optimal concentration of 12 °Bx. Using single factor and orthogonal experiments, the optimal culture conditions were determined: optimum inoculum size, 15%; initial pH, 6.0; culture time, 8 days; rotation speed, 180 rpm; and temperature, 28 °C. Under the optimal conditions, the humic acid production rate for the strain was 49.50 g/L. The results of characterization and infrared spectral analysis revealed that the produced humic acid contained a variety of reactive functional groups.

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Funding

This work was supported in part by Guangxi Special Funds for Bagui Scholars (2013-03), Project of Guangxi Sugarcane Innovation team of National Modern Agriculture Industry Technology System (gjnytxgxcxtd-03).

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Author notes

  1. Nan Li and Zhi-Nian Deng have contributed equally to this work.

Authors and Affiliations

  1. College of Life Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China

    Nan Li

  2. Guangxi Crop Genetic Improvement and Biotechnology Laboratory, China Ministry of Agriculture Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Guangxi Key Laboratory of Sugarcane Genetic Improvement, Guangxi Academy of Agricultural Sciences, Nanning, 530007, Guangxi, China

    Zhi-Nian Deng, Yuan-Wen Wei, Hui-Qing Cao & Yang-Rui Li

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  1. Nan Li
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  2. Zhi-Nian Deng
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  3. Yuan-Wen Wei
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  4. Hui-Qing Cao
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  5. Yang-Rui Li
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Correspondence to Yang-Rui Li.

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Li, N., Deng, ZN., Wei, YW. et al. Production of Humic Acid by a Bacillus megaterium Strain Using Vinasse. Sugar Tech 20, 163–167 (2018). https://doi.org/10.1007/s12355-017-0554-2

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  • DOI: https://doi.org/10.1007/s12355-017-0554-2

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