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Unyvero ITI® system for the clinical resolution of discrepancies in periprosthetic joint infection diagnosis

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Abstract

Objectives

The Unyvero molecular assay was tested for the clinical resolution of discordant results, evaluating its role in prosthetic joint infection diagnosis.

Methods

Multiplex PCR was performed on 45 samples from prosthesis treatment (either sonication or dithiothreitol). Analytical performance was compared to that of biofilm culture using Musculoskeletal Infection Society criteria as gold standard.

Results

Unyvero and biofilm culture showed similar agreement rates compared to the gold standard (34/43 and 32/43, respectively). Both methods showed six additional identifications compatible with true infection; five positive results from biofilm culture were deemed contaminations.

Conclusions

The Unyvero system showed good performances and a significantly shorter turnaround time compared to cultural methods, presenting an added value to PJI diagnosis even when performed following a composite approach.

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Acknowledgements

The authors wish to thank Curetis® for providing free of charge the devices and consumables necessary for the study as a trial run. The company played no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Unit of Microbiology, The Great Romagna Area Hub Laboratory, Pievesestina, Cesena, Italy

    Silvia Zannoli, Manuela Morotti, Martina Tassinari, Arianna Torri & Vittorio Sambri

  2. Department of Orthopaedic Oncology, Istituto Ortopedico Rizzoli, Via Pupilli 1, 40136, Bologna, Italy

    Andrea Sambri, Giuseppe Bianchi, Massimiliano De Paolis & Davide Maria Donati

  3. Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Bologna, Italy

    Vittorio Sambri

  4. Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy

    Andrea Sambri & Davide Maria Donati

Authors
  1. Silvia Zannoli
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  2. Andrea Sambri
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  3. Manuela Morotti
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  4. Martina Tassinari
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  5. Arianna Torri
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  6. Giuseppe Bianchi
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  7. Massimiliano De Paolis
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  8. Davide Maria Donati
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  9. Vittorio Sambri
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Corresponding author

Correspondence to Andrea Sambri.

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Conflict of interest

All authors declare no conflicts of interest and no competing financial interests.

Ethical approval

Ethical approval or informed consent was not required, since the study has been performed using exclusively anonymized leftover samples derived from a previous study. The anonymization was achieved by using the current procedure (AVR-PPC P09, rev.2) checked by the local Ethical Board.

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Zannoli, S., Sambri, A., Morotti, M. et al. Unyvero ITI® system for the clinical resolution of discrepancies in periprosthetic joint infection diagnosis. Musculoskelet Surg 105, 39–42 (2021). https://doi.org/10.1007/s12306-019-00626-x

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  • DOI: https://doi.org/10.1007/s12306-019-00626-x

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