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Identification of modifiers of the plant height in wheat using an induced dwarf mutant controlled by RhtB4c allele

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Abstract

In wheat, 25 Rht genes for dwarfness are known, which include both, GA-insensitive and GA-responsive genes. The GA-insensitive Rht genes have been widely used, although, their suitability under abiotic stress conditions has been questioned. This necessitated a search for alternative GA-responsive, spontaneous and induced dwarfing genes. We earlier reported an induced dwarf mutant (‘dwarf mutant-3′; 44 cm), and the mutant allele was named Rht4c allele (2BL). This dwarf mutant was not suitable for cultivation due to its extra dwarf nature. Therefore, we searched for naturally occurring QTLs, which would modify the phenotype of ‘dwarf-mutant-3′ using ‘mutant-assisted gene identification and characterization’ (MAGIC) approach. For this purpose, the ‘dwarf mutant-3′ was crossed with a tall wheat cv. NP114 and homozygous mutant F2 plants (~ 25% of the progeny) were selected, which were phenotyped for plant height and genotyped using SSR markers. The data were utilized for QTL analysis and plant height. Six modifier QTLs were identified on chromosomes 2A, 2B and 4A. Two QTLs each on 2A and 2B were responsible for increase in plant height (described as ‘enhancer modifiers’), whereas the remaining two QTLs located on 4A were responsible for reducing the plant height (described as ‘suppressor modifiers’). It was hypothesized that the enhancer QTLs could be exploited for the development of semi-dwarf high yielding genotypes containing Rht4c allele. This is the first study of its kind in wheat demontsrating that the MAGIC approach could be used for identification of modifiers of the mutant phenotypes of other traits for wheat improvement.

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Acknowledgements

HSB was awarded the Senior Scientist position by INSA, New Delhi. Thanks are due to Head of the Department for providing facilities.

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  1. Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut, 250004, India

    Priyanka Agarwal, H. S. Balyan & P. K. Gupta

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  1. Priyanka Agarwal
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  2. H. S. Balyan
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  3. P. K. Gupta
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PKG and HSB conceived the study and wrote the manuscript. PA conducted the field and laboratory experiment and analysed the data.

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Correspondence to P. K. Gupta.

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Agarwal, P., Balyan, H.S. & Gupta, P.K. Identification of modifiers of the plant height in wheat using an induced dwarf mutant controlled by RhtB4c allele. Physiol Mol Biol Plants 26, 2283–2289 (2020). https://doi.org/10.1007/s12298-020-00904-0

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