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Growth, physiological and proteomic responses in field grown wheat varieties exposed to elevated CO2 under high ambient ozone

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Abstract

The present study investigated growth, biochemical, physiological, yield and proteomic changes in 3 wheat varieties exposed to elevated CO2 (515 ppm) in a background of high ambient ozone in field. Ethylenediurea (EDU) was used as antiozonant. Average ozone concentration was 59 ppb and was sufficient enough to exert phytotoxic effects. Elevated carbon dioxide (eCO2) and EDU application individually or in combination negated the adverse effects of ozone by modulating antioxidants and antioxidative enzymes. Differential leaf proteomics revealed that at vegetative stage major changes in protein abundance were due to EDU treatment (47, 52 and 41 proteins in PBW-343, LOK1 and HD-2967, respectively). Combined treatment of eCO2 and EDU was more responsible for changes in 37 proteins during flowering stage of PBW-343 and LOK1. Functional categorization revealed more than 60% differentially abundant protein collectively belonging to carbon metabolism, protein synthesis assembly and degradation and photosynthesis. At both the growth stages, LOK1 was more responsive to eCO2 and combined treatment (eCO2 + EDU). HD-2967 was more positively responsive to EDU and combined treatment. eCO2 in combination of EDU protected these varieties against high ambient O3.

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Acknowledgements

Authors are grateful to Director, CSIR-NBRI, Lucknow, India for providing all necessary facilities. Funding for this work was provided by Council of Scientific & Industrial Research (CSIR), New Delhi, India (Project Nos. PSC 112 and BSC 109).

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Authors and Affiliations

  1. Plant Ecology and Climate Change Science Division, CSIR-National Botanical Research Institute, Lucknow, 226001, India

    Vivek K. Maurya, Sunil K. Gupta, Marisha Sharma, Baisakhi Majumder, Farah Deeba & Vivek Pandey

  2. Department of Botany, University of Lucknow, Lucknow, 226001, India

    Vivek K. Maurya & Nalini Pandey

  3. Biotechnology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226015, India

    Farah Deeba

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  1. Vivek K. Maurya
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  2. Sunil K. Gupta
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Contributions

VP, NP and VKM designed the experiment. VKM, BM and MS did yield and physiological work. VKM and BM did the enzymatic and biochemical analysis. VK, SKG, MS and FD did proteomic work. VKM and VP analysed the data. VKM, NP, FD and VP wrote the paper. All the authors approved the paper.

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Correspondence to Vivek Pandey.

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Maurya, V.K., Gupta, S.K., Sharma, M. et al. Growth, physiological and proteomic responses in field grown wheat varieties exposed to elevated CO2 under high ambient ozone. Physiol Mol Biol Plants 26, 1437–1461 (2020). https://doi.org/10.1007/s12298-020-00828-9

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