Abstract
Abiotic stresses negatively influence the survival, biomass production, and yield of crops. Tolerance to diverse abiotic stresses in plants is regulated by multiple genes responding differently to various stress conditions. Genetic engineering approaches have helped develop transgenic crops with improved abiotic stress tolerance including yields. The dehydration-responsive element binding protein (DREB) is a stress-responsive transcription factor that modulates the expression of downstream stress-inducible genes, which confer simultaneous tolerance to multiple stresses. This review focuses on advances in the development of DREB transgenic crops and their characterization under various abiotic stress conditions. It further discusses the mechanistic aspects of abiotic stress tolerance, yield gain, the fate of transgenic plants under controlled and field conditions and future research directions toward commercialization of DREB transgenic crops.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Agarwal PK, Jha B (2010) Transcription factors in plants and ABA-dependent and independent abiotic stress signaling. Biol Plant 54(2):201–212. https://doi.org/10.1007/s10535-010-0038-7
Agarwal PK, Agarwal P, Reddy MK, Sopory SK (2006) Role of DREB transcription factors in abiotic and biotic stress tolerance in plants. Plant Cell Rep 25:263–1274. https://doi.org/10.1007/s00299-006-0204-8
Agarwal P, Agarwal PK, Joshi AJ, Sopory SK, Reddy MK (2010) Overexpression of PgDREB2A transcription factor enhances abiotic stress. Mol Biol Rep 37:1125–1135. https://doi.org/10.1007/s11033-009-9885-8
Agarwal PK, Gupta K, Lopato S, Agarwal P (2017) Dehydration responsive element binding transcription factors and their applications for the engineering of stress tolerance. J Exp Bot 68(9):2135–2148. https://doi.org/10.1093/jxb/erx118
Akhtar M, Jaiswal A, Taj G, Jaiswal JP, Qureshi MI, Singh NK (2012) DREB1/CBF transcription factors: their structure, function and role in abiotic stress tolerance in plants. J Genet 91(3):385–395. https://doi.org/10.1007/s12041-012-0201-3
Al-Abed D, Madasamy P, Talla R, Goldman S, Rudrabhatla S (2007) Genetic engineering of maize with the Arabidopsis DREB1A/CBF3 gene using split-seed explants. Crop Sci 47:2390–2401. https://doi.org/10.2135/cropsci2006.11.0712
Anbazhagan K, Bhatnagar-Mathur P, Sharma KK, Baddam R, Kavi Kishor PB, Vadez V (2015a) Changes in timing of water uptake and phenology favours yield gain in terminal water stressed chickpea AtDREB1A transgenics. Funct Plant Biol 42:84–94. https://doi.org/10.1071/FP14115
Anbazhagan K, Bhatnagar-Mathur P, Vadez V, Dumbala SR, Kavi Kishor PB, Sharma KK (2015b) DREB1A overexpression in transgenic chickpea alters key traits influencing plant water budget across water regimes. Plant Cell Rep 34:199–210. https://doi.org/10.1007/s00299-014-1699-z
Ban Q, Liu G, Wang Y (2011) A DREB gene from Limonium bicolour mediates molecular and physiological responses to copper stress in transgenic tobacco. J Plant Physiol 168(5):449–458. https://doi.org/10.1016/j.jplph.2010.08.013
Bhatnagar-Mathur P, Devi MJ, Reddy DS, Lavanya M, Vadez V, Serraj R, Yamaguchi-Shinozaki K, Sharma KK (2007) Stress-inducible expression of AtDREB1A in transgenic peanut (Arachis hypogaea L.) increases transpiration efficiency under water-limiting conditions. Plant Cell Rep 26:2071–2082. https://doi.org/10.1007/s00299-007-0406-8
Bhatnagar-Mathur P, Vadez V, Sharma KK (2008) Transgenic approaches for abiotic stress tolerance in plants: retrospect and prospects. Plant Cell Rep 27:411–424. https://doi.org/10.1007/s00299-007-0474-9
Bhatnagar-Mathur P, Devi MJ, Vadez V, Sharma KK (2009) Differential antioxidative responses in transgenic peanut bear no relationship to their superior transpiration efficiency under drought stress. J Plant Physiol 166:1207–1217. https://doi.org/10.1016/j.jplph.2009.01.001
Bhatnagar-Mathur P, Rao JS, Vadez V, Sharma KK (2010) Transgenic strategies for improved drought tolerance in legumes of semi-arid tropics. J Crop Improv 24(1):92–111. https://doi.org/10.1080/15427520903337095
Bhatnagar-Mathur P, Rao JS, Vadez V, Dumbala SR, Rathore A, Yamaguchi-Shinozaki K, Sharma KK (2014) Transgenic peanut overexpressing the DREB1A transcription factor has higher yields under drought stress. Mol Breed 33:327–340. https://doi.org/10.1007/s11032-013-9952-7
Bosamia TC, Mishra GP, Radhakrishnan T, Dobaria JR (2015) Novel and stress relevant EST derived SSR markers developed and validated in peanut. PLoS ONE 10(6):e0129127. https://doi.org/10.1371/journal.pone.0129127
Bouaziz D, Pirrello J, Ben Amor H, Hammami A, Charfeddine M, Dhieb A, Bouzayen M, Gargouri-Bouzid R (2012) Ectopic expression of dehydration responsive element binding proteins (StDREB2) confers higher tolerance to salt stress in potato. Plant Physiol Biochem 60:98–108. https://doi.org/10.1016/j.plaphy.2012.07.029
Bouaziz D, Pirrello J, Charfeddine M, Hammami A, Jbir R, Dhieb A, Bouzayen M, Gargouri-Bouzid R (2013) Overexpression of StDREB1 transcription factor increases tolerance to salt in transgenic potato plants. Mol Biotechnol 54(3):803–817. https://doi.org/10.1007/s12033-012-9628-2
Charfeddine M, Bouaziz D, Charfeddine S, Hammami A, Ellouz ON, Bouzid RG (2015) Overexpression of dehydration-responsive element-binding 1 protein (DREB1) in transgenic Solanum tuberosum enhances tolerance to biotic stress. Plant Biotechnol Rep 9:79–88. https://doi.org/10.1007/s11816-015-0345-8
Chen JR, Lü JJ, Rong L, Xiong XY, Wang TX, Chen SY, Guo LB, Wang HF (2010) DREB1C from Medicago truncatula enhances freezing tolerance in transgenic M. truncatula and china rose (Rosa chinensis Jacq.). Plant Growth Reg 60(3):199–211. https://doi.org/10.1007/s10725-009-9434-4
Chinnusamy V, Ohta M, Kanrar S, Lee BH, Hong X, Agarwal M, Zhu JK (2003) ICE1, a regulator of cold induced transcriptome and freezing tolerance in Arabidopsis. Genes Dev 17:1043–1054. https://doi.org/10.1101/gad.1077503
Datta K, Baisakh N, Ganguly M, Krishnan S, Shinozaki KY, Datta SK (2012) Overexpression of Arabidopsis and rice stress genes inducible transcription factor confers drought and salinity tolerance to rice. Plant Biotechnol J 10:579–586. https://doi.org/10.1111/j.1467-7652.2012.00688.x
de Paiva Rolla AA, de Fátima Corrêa Carvalho J, Fuganti-Pagliarini R, Engels C, do Rio A, Marin SRR, de Oliveira MCN, Beneventi MA, Marcelino-Guimarães FC, Farias JRB, Neumaier N, Nakashima K, Yamaguchi-Shinozaki K, Nepomuceno AL (2014) Phenotyping soybean plants transformed with rd29A: AtDREB1A for drought tolerance in the greenhouse and field. Transgen Res 23(1):75–87. https://doi.org/10.1007/s11248-013-9723-6
Devi MJ, Bhatnagar-Mathur P, Sharma KK, Serraj R, Anwar SY, Vadez V (2011) Relationships between transpiration efficiency and its surrogate traits in the rd29A:DREB1A transgenic lines of groundnut. J Agron Crop Sci 197(4):249–328. https://doi.org/10.1111/j.1439-037X.2011.00464.x
Dong C, Ma Y, Wisniewski M, Cheng ZM (2017) Meta-analysis of the effect of overexpression of CBF/DREB family genes on drought stress response. Environ Exp Bot 142:1–4. https://doi.org/10.1016/j.envexpbot.2017.07.014
Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009) Plant drought stress: effects, mechanisms and management. Agron Sustain Dev 29(1):185–212. https://doi.org/10.1051/agro:2008021
Gao SQ, Chen M, Xia LQ, Xiu HJ, Xu ZS, Li LC, Zhao CP, Cheng XG, Ma YZ (2009) A cotton (Gossypium hirsutum) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat. Plant Cell Rep 28:301–311. https://doi.org/10.1007/s00299-008-0623-9
Gutha LR, Reddy AR (2008) Rice DREB1B promoter shows distinct stress- specific responses, and the overexpression of cDNA in tobacco confers improved abiotic and biotic stress tolerance. Plant Mol Biol 68:533–555. https://doi.org/10.1007/s11103-008-9391-8
Hackenberg M, Shi B-J, Gustafson P, Langridge P (2012) A transgenic transcription factor (TaDREB3) in barley affects the expression of microRNAs and other small non-coding RNAs. PLoS ONE 7(8):e42030. https://doi.org/10.1371/journal.pone.0042030
Hong B, Ma C, Yang Y, Wang T, Yamaguchi-Shinozaki K, Gao J (2009) Over-expression of AtDREB1A in chrysanthemum enhances tolerance to heat stress. Plant Mol Biol 70:231–240. https://doi.org/10.1007/s11103-009-9468-z
Jagana SR, Vadez V, Bhatnagar-Mathur P, Narasu ML, Sharma KK (2012) Better root:shoot ratio conferred enhanced harvest index in transgenic groundnut overexpressing the rd29A:DREB1A gene under intermittent drought stress in an outdoor lysimetric dry-down trial. J SAT Agric Res 10:1–7
Jiang Q, Hu Z, Zhang H, Ma Y (2014) Overexpression of GmDREB1improves salt tolerance in transgenic wheat and leaf protein response to high salinity. Crop J 2:120–131. https://doi.org/10.1016/j.cj.2014.02.003
Jiang Q, Sun X, Niu F, Hu Z, Chen R, Zhang H (2017) GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds. PLoS ONE 12(5):e0175924. https://doi.org/10.1371/journal.pone.0175924
Jin T, Chang Q, Li W, Yin D, Li Z, Wang D, Liu B, Liu L (2010) Stress-inducible expression of GmDREB1 conferred salt tolerance in transgenic alfalfa. Plant Cell Tissue Organ Cult 100:219–227. https://doi.org/10.1007/s11240-009-9628-5
Jin R, Kim BH, Ji CY, Kim HS, Li HM, Kwak SS (2017) Overexpressing IbCBF3 increases low temperature and drought stress tolerance in transgenic sweetpotato. Plant Physiol Biochem 118:45–54. https://doi.org/10.1016/j.plaphy.2017.06.002
Lata C, Bhutty S, Bahadur RP, Majee M, Prasad M (2011) Association of an SNP in a novel DREB2-like gene SiDREB2 with stress tolerance in foxtail millet [Setaria italica (L.)]. J Exp Bot 62(10):3387–3401. https://doi.org/10.1093/jxb/err016
Lee H, Xiong L, Gong Z, Ishitani M, Stevenson B, Zhu JK (2001) The Arabidopsis HOS1 gene negatively regulates cold signal transduction and encodes a RING finger protein that displays cold-regulated nucleo-cytoplasmic partitioning. Genes Dev 15:912–924. https://doi.org/10.1101/gad.866801
Li X, Cheng X, Liu J, Zeng H, Han L, Tang W (2011) Heterologous expression of the Arabidopsis DREB1A/CBF3 gene enhances drought and freezing tolerance in transgenic Lolium perenne plants. Plant Biotech Rep 5(1):61–69. https://doi.org/10.1007/s11816-010-0157-9
Li J, Sima W, Ouyang B, Wang T, Ziaf K, Luo Z, Liu L, Li H, Chen M, Huang Y, Feng Y (2012) Tomato SlDREB gene restricts leaf expansion and internode elongation by downregulating key genes for gibberellin biosynthesis. J Exp Bot 63(18):6407–6420. https://doi.org/10.1093/jxb/ers295
Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought-and low-temperature-responsive gene expression, respectively in Arabidopsis. Plant Cell 10(8):1391–1406. https://doi.org/10.1105/tpc.10.8.1391
Liu Y, Zhang M-H, Yu Y-B, Sun Z, Zhen Z, Gao X-J (2014) Event-specific qualitative and quantitative detection in transgenic soybean OsDREB3 based on the 5′ flanking sequence. Food Biotechnol 28(1):63–78. https://doi.org/10.1080/08905436.2013.871289
Mishra GP, Singh B, Seth T, Singh AK, Halder J, Krishnan N, Tiwari SK, Singh PM (2017) Biotechnological advancements and Begomovirus management in okra (Abelmoschus esculentus L.): status and perspectives. Front Plant Sci 8:360. https://doi.org/10.3389/fpls.2017.00360
Morran S, Eini O, Pyvovarenko T, Parent B, Singh R, Ismagul A, Eliby S, Shirley N, Langridge P, Lopato S (2011) Improvement of stress tolerance of wheat and barley by modulation of expression of DREB/CBF factors. Plant Biotechnol J 9:230–249. https://doi.org/10.1111/j.1467-7652.2010.00547.x
Movahedi S, Sayed TBE, Alizade H, Ghobadi C, Yamchi A, Khaksar G (2012) Constitutive expression of Arabidopsis DREB1B in transgenic potato enhances drought and freezing tolerance. Biol Plantarum 56(1):37–42. https://doi.org/10.1007/s10535-012-0013-6
Narusaka Y, Nakashima K, Shinwari ZK, Sakuma Y, Furihata T (2003) Interaction between two cis-acting elements, ABRE and DRE, in ABA-dependent expression of Arabidopsis rd29A gene in response to dehydration and high salinity stresses. Plant J 34:137–148. https://doi.org/10.1046/j.1365-313X.2003.01708.x
Oh SJ, Song SI, Kim YS, Jang HJ, Kim SY, Kim M, Kim YK, Nahm BH, Kim JK (2005) Arabidopsis CBF3/DREB1A and ABF3 in transgenic rice increased tolerance to abiotic stress without stunting growth. Plant Physiol 138:341–351. https://doi.org/10.1104/pp.104.059147
Oh SJ, Kwon C-W, Choi D-W, Song SI, Kim JK (2007) Expression of barley HvCBF4 enhances tolerance to abiotic stress in transgenic rice. Plant Biotechnol J 5:646–656. https://doi.org/10.1111/j.1467-7652.2007.00272.x
Oraby H, Ahmad R (2012) Physiological and biochemical changes of CBF3 transgenic oat in response to salinity stress. Plant Sci 185–186:331–339. https://doi.org/10.1016/j.plantsci.2012.01.003
Patel KG, Mandaliya VB, Mishra GP, Dobaria JR, Radhakrishnan T (2016) Transgenic peanut overexpressing mtlD gene confers enhanced salinity-stress tolerance via mannitol accumulation and differential antioxidative responses. Acta Physiol Plant 38:181. https://doi.org/10.1007/s11738-016-2200-0
Patel K, Thankappan R, Mishra GP, Mandalia V, Kumar A, Dobaria JR (2017) Transgenic peanut (Arachis hypogaea L.) overexpressing mtlD gene showed improved photosynthetic, physio-biochemical and yield-parameters under soil-moisture deficit stress in lysimeter system. Front Plant Sci. https://doi.org/10.3389/fpls.2017.01881
Paul S, Gayen D, Datta SK, Datta K (2015) Dissecting root proteome of transgenic rice cultivars unravels metabolic alterations and accumulation of novel stress responsive proteins under drought stress. Plant Sci 234:133–143. https://doi.org/10.1016/j.plantsci.2015.02.006
Pellegrineschi A, Reynolds M, Pacheco M, Brito RM, Almeraya R, Yamaguchi-Shinozaki K, Hoisington D (2004) Stress-induced expression in wheat of the Arabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions. Genome 47:493–500. https://doi.org/10.1139/g03-140
Pruthvi V, Narasimhan R, Nataraja KN (2014) Simultaneous expression of abiotic stress responsive transcription factors, AtDREB2A, AtHB7and AtABF3 improves salinity and drought tolerance in peanut (Arachis hypogaea L.). PLoS ONE 9(12):e111152. https://doi.org/10.1371/journal.pone.011115
Rai GK, Rai NP, Rathaur S, Kumar S, Major S (2013) Expression of rd29A:AtDREB1A/CBF3 in tomato alleviates drought-induced oxidative stress by regulating key enzymatic and non-enzymatic antioxidants. Plant Physiol Biochem 69:90–100. https://doi.org/10.1016/j.plaphy.2013.05.002
Rao ES, Kadirvel P, Symonds RC, Geethanjali S, Thontadarya RN, Ebert AW (2015) Variations in DREB1A and VP1.1 genes show association with salt tolerance traits in wild tomato (Solanum pimpinellifolium). PLoS ONE 10(7):e0132535. https://doi.org/10.1371/journal.pone.0132535
Ravikumar G, Manimaran P, Voleti SR, Subrahmanyam D, Sundaram RM, Bansal KC, Viraktamath BC, Balachandran SM (2014) Stress-inducible expression of AtDREB1A transcription factor greatly improves drought stress tolerance in transgenic indica rice. Transgen Res 23(3):421–439. https://doi.org/10.1007/s11248-013-9776-6
Reis RR, da Cunha BADB, Martinsa PK, Martinsa MTB, Alekcevetch JC, Chalfun-Júnior A, Andrade AC, Ribeiro AP, Qind F, Mizoie J, Yamaguchi-Shinozaki K, Nakashima K, Carvalho JDFC, de Sousa CAF, Nepomucenof AL, Kobayashi AK, Molinari HBC (2014) Induced over-expression of AtDREB2A CA improves drought tolerance in sugarcane. Plant Sci 221:59–68. https://doi.org/10.1016/j.plantsci.2014.02.003
Sakuma Y, Maruyama K, Qin F, Osakabe Y, Shinozaki K, Yamaguchi-Shinozaki K (2006) Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression. Proc Natl Acad Sci USA 103(49):18822–18827. https://doi.org/10.1073/pnas.0605639103
Sarkar T (2014) Development of transgenic resistance to abiotic stress in groundnut using AtDREB1A gene through Agrobacterium mediated genetic transformation. Ph.D. Thesis, Saurashtra University, Rajkot, Gujarat, India
Sarkar T, Radhakrishnan T, Kumar A, Mishra GP, Dobaria JR (2014) Heterologus expression of AtDREB1A gene in transgenic peanut conferred tolerance to drought and salinity stresses. PLoS ONE 9(12):e110507. https://doi.org/10.1371/journal.pone.0110507
Sarkar T, Radhakrishnan T, Kumar A, Mishra GP, Dobaria JR (2016) Stress inducible expression of AtDREB1A transcription factor in transgenic peanut (Arachis hypogaea L.) conferred tolerance to soil-moisture deficit stress. Front Plant Sci 7:935. https://doi.org/10.3389/fpls.2016.00935
Sarkar T, Mogili T, Sivaprasad V (2017) Improvement of abiotic stress adaptive traits in mulberry (Morus spp.): an update on biotechnological interventions. 3 Biotech 7:214. https://doi.org/10.1007/s13205-017-0829-z
Schramm F, Larkindale J, Kiehlmann E, Ganguli A, Englich G, Elizabeth Vierling E, Koskull-Doäring PV (2008) A cascade of transcription factor DREB2A and heat stress transcription factor HsfA3 regulates the heat stress response of Arabidopsis. Plant J 53:264–274. https://doi.org/10.1111/j.1365-313X.2007.03334.x
Shah SH, Ali S, Jan SA, Jalal-Ud-Din Ali GM (2015) Piercing and incubation method of in planta transformation producing stable transgenic plants by overexpressing DREB1A gene in tomato (Solanum lycopersicum Mill.). Plant Cell Tissue Organ Cult 120:1139–1157. https://doi.org/10.1007/s11240-014-0670-6
Shavrukov Y, Baho M, Lopato S, Langridge P (2016) The TaDREB3 transgene transferred by conventional crossings to different genetic backgrounds of bread wheat improves drought tolerance. Plant Biotechnol J 14:313–322. https://doi.org/10.1111/pbi.12385
Tang Y, Liu K, Zhang J, Li X, Xu K, Zhang Y, Qi J, Yu D, Wang J, Lil C (2017) JcDREB2, a physic nut AP2/ERF gene, alters plant growth and salinity stress responses in transgenic rice. Front Plant Sci 8:306. https://doi.org/10.3389/fpls.2017.00306
Tsutsui T, Kato W, Asada Y, Sako K, Sato T, Sonoda Y, Kidokoro S, Yamaguchi-Shinozaki K, Tamaoki M, Arakawa K, Ichikawa T, Nakazawa M, Seki M, Shinozaki K, Matsui M, Ikeda A, Yamaguchi J (2009) DEAR1, a transcriptional repressor of DREB protein that mediates plant defense and freezing stress responses in Arabidopsis. J Plant Res 122:633–643. https://doi.org/10.1007/s10265-009-0252-6
Vadez V, Rao S, Sharma KK, Bhatnagar-Mathur P, Jyotsna DM (2007) DREB1A allows for more water uptake in groundnut by a large modification in the root/shoot ratio under water deficit. J SAT Agric Res 5(1):1–5
Wang F, Gao ZH, Qiao YS, Mi L, Li JF, Zhang Z, Lin Z-L, Gu X-B (2014) RDREB1BI gene expression driven by the stress-induced promoter RD29A enhances tolerance to cold stress in benihope strawberry. In: ISHS acta horticulturae 1049: VII international strawberry symposium. https://doi.org/10.17660/actahortic.2014.1049.159
Wang H, Wang H, Shao H, Tang X (2016) Recent advances in utilizing transcription factors to improve plant abiotic stress tolerance by transgenic technology. Front Plant Sci 7:67. https://doi.org/10.3389/fpls.2016.00067
Wei S, Sun Z, Han L, Yu L (2006) Transgenic tobacco plants over-expressing Arabidopsis transcriptional factor CBF1 show morphological and biochemical characteristics associated with cold tolerance. Asian J Plant Sci 5:932–939. https://doi.org/10.3923/ajps.2006.932.939
Wei T, Deng K, Gao Y, Liu Y, Yang M, Zhang L, Zheng X, Wang C, Song W, Chen C, Zhang Y (2016) Arabidopsis DREB1B in transgenic Salvia miltiorrhiza increased tolerance to drought stress without stunting growth. Plant Physiol Biochem 104:17–28. https://doi.org/10.1016/j.plaphy.2016.03.003
Yamaguchi-Shinozaki K, Shinozaki K (1994) A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell 6:251–264. https://doi.org/10.1105/tpc.6.2.251
Yang JS, Wang R, Meng JJ, Bi YP, Xu PL, Guo F, Wan SB, He QW, Li XG (2010) Overexpression of Arabidopsis CBF1 gene in transgenic tobacco alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance. J Plant Physiol 167(7):534–539. https://doi.org/10.1016/j.jplph.2009.11.005
Yang S, Tang XF, Ma NN, Wang LY, Meng QW (2011a) Heterology expression of the sweet pepper CBF3 gene confers elevated tolerance to chilling stress in transgenic tobacco. J Plant Physiol 168(15):1804–1812. https://doi.org/10.1016/j.jplph.2011.05.017
Yang W, Liu XD, Chi XJ, Wu CA, Li YZ, Song LL, Liu XM, Wang YF, Wang FW, Zhang C, Liu Y (2011b) Dwarf apple MbDREB1 enhances plant tolerance to low temperature, drought, and salt stress via both ABA-dependent and ABA-independent pathways. Planta 233(2):219–229. https://doi.org/10.1007/s00425-010-1279-6
Zhang S, Li N, Gao F, Yang A, Zhang J (2010) Over-expression of TsCBF gene confers improved drought tolerance in transgenic maize. Mol Breed 26:455–465. https://doi.org/10.1007/s11032-009-9385-5
Zhang X-X, Tang Y-J, Ma O-B, Yang C-Y, Mu Y-H, Suo H-C et al (2013) OsDREB2A, a rice transcription factor, significantly affects salt tolerance in transgenic soybean. PLoS ONE 8(12):e83011. https://doi.org/10.1371/journal.pone.0083011
Zhao L, Hu Y, Chong K, Wang T (2010) ARAG1, an ABA-responsive DREB gene, plays a role in seed germination and drought tolerance of rice. Ann Bot 105:401–409. https://doi.org/10.1093/aob/mcp303
Zhuang J, Wang F, Xu Z-S, Xiong A-S (2015) Microarray analysis of different expression profiles between wild-type and transgenic rice seedlings overexpression OsDREB1BI gene. Biologia 70(6):760–770. https://doi.org/10.1515/biolog-2015-0092
Zong J-M, Li X-W, Zhou Y-H, Wang F-W, Wang N, Dong Y-Y, Yuan Y-X, Chen H, Liu X-M, Yao N, Li H-Y (2016) The AaDREB1 transcription factor from the cold-tolerant plant Adonis amurensis enhances abiotic stress tolerance in transgenic plant. Int J Mol Sci 17:611. https://doi.org/10.3390/ijms17040611
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflicts of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Sarkar, T., Thankappan, R., Mishra, G.P. et al. Advances in the development and use of DREB for improved abiotic stress tolerance in transgenic crop plants. Physiol Mol Biol Plants 25, 1323–1334 (2019). https://doi.org/10.1007/s12298-019-00711-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12298-019-00711-2