Skip to main content
SpringerLink
Log in

Physiological performance of two contrasting rice varieties under water stress

  • Research Article
  • Published:
Physiology and Molecular Biology of Plants Aims and scope Submit manuscript

Abstract

Two rice varieties PR-115 and Super-7 were imposed to water stress and different physiological traits were monitored to evaluate the performance of these varieties under drought. Under water stress condition although the relative water content, osmotic potential, chlorophyll content, photosynthesis rate, carbon discrimination and biomass decreased in both the varieties however, the reduction was more pronounced in Super-7 variety. Oryzanol a trans-ester of ferulic acid functions as antioxidant and it increased along with total phenolic and anthocyanin content in both the varieties under drought stress. However, gallic acid, 4 hydroxy benzoic acid, syringic acid and chlorogenic acid showed differential pattern in both of the varieties under water limiting conditions. Under drought, grain yield was penalized by 17 and 54% in PR-115 and Super-7 varieties, respectively in comparison to watered plants. Super-7 variety showed pronounced electrolyte leakage and MDA enhancement under water stress condition. High non photochemical quenching and reduction in Y(NO) and Y(II) indicated balanced energy management in tolerant PR-115 variety. The studies showed that PR-115 is a drought tolerant variety while Super-7 is drought sensitive in nature.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

Abbreviations

∆13C:

Carbon isotope discrimination

δ13C:

13C/12C ratio relative to the CO2 of atmosphere

A :

Net photosynthesis rate

DW:

Dry weight

E :

Transpiration

EC:

Electrical conductivity

ETR:

Electron transport rate

Fv/Fm:

Maximum quantum yield of PSII

FW:

Fresh weight

g s :

Stomatal conductance

IRMS:

Isotopic ratio mass spectroscopy

MDA:

Malondialdehyde

PS:

Photosystem

ROS:

Reactive oxygen species

RWC:

Relative water content

TPC:

Total phenolic content

TW:

Turgid weight

WUE:

Water use efficiency

Y(I):

Photochemical quantum yield of energy conversion of PSI

Y(II):

Photochemical quantum yield of PSII

Y(NA):

Photochemical quantum yield of PSI due to acceptor side limitation

Y(ND):

Photochemical quantum yield of PSI due to donor side limitation

Y(NPQ):

Regulated energy dissipation

Y(NO):

Non-regulated energy dissipation

References

  • Adiredjo AL, Navaud O, Muños S, Langlade NB, Lamaze T, Grieu P (2014) Genetic control of water use efficiency and leaf carbon isotope discrimination in sunflower (Helianthus annuus L.) subjected to two drought scenarios. PLoS One 9(7):e101218

    Article  PubMed  PubMed Central  Google Scholar 

  • Araus JL, Slafer GA, Reynolds MP, Royo C (2002) Plant breeding and drought in C3 cereals: what should we breed for? Ann Bot 89:925–940

    Article  PubMed  PubMed Central  Google Scholar 

  • Baker NR (2008) Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annu Rev Plant Biol 59:89–113

    Article  CAS  PubMed  Google Scholar 

  • Ball RA, Oosterhuis DM (2005) Measurement of root and leaf osmotic potential using the vapor-pressure osmometer. Env Exp Bot 53:77–84

    Article  Google Scholar 

  • Blum A (2005) Drought resistance, water-use efficiency, and yield potential-are they compatible, dissonant, or mutually exclusive? Crop Pasture Sci 56:1159–1168

    Article  Google Scholar 

  • Blum A (2009) Effective use of water (EUW) and not water-use efficiency (WUE) is the target of crop yield improvement under drought stress. Field Crop Res 112:119–123

    Article  Google Scholar 

  • Britz SJ, Prasad PVV, Moreau RA, Allen LH, Kremer DF, Boote KJ (2007) Influence of growth temperature on the amounts of tocopherols, tocotrienols, and γ-oryzanol in brown rice.J Agr. Food Chem 55:7559–7565

    Article  CAS  Google Scholar 

  • Cattivelli L, Rizza F, Badeck FW, Mazzucotelli E, Mastrangelo AM, Francia E, Stanca AM (2008) Drought tolerance improvement in crop plants: an integrated view from breeding to genomics. Field Crop Res 105:1–14

    Article  Google Scholar 

  • Centritto M, Lauteri M, Monteverdi MC, Serraj R (2009) Leaf gas exchange, carbon isotope discrimination, and grain yield in contrasting rice genotypes subjected to water deficits during the reproductive stage. J Exp Bot 60:2325–2339

    Article  CAS  PubMed  Google Scholar 

  • Chaves MM, Maroco JP, Pereira JS (2003) Understanding plant responses to drought-from genes to the whole plant. Funct Plant Biol 30:239–264

    Article  CAS  Google Scholar 

  • Chaves MM, Flexas J, Pinheiro C (2009) Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Ann Bot 103:551–560

    Article  CAS  PubMed  Google Scholar 

  • Cheruvanky R (2003) Phytochemical products: rice bran. In: Johnson I, Williamson G (eds) Phytochemical functional foods. Woodhead Publishing, Sawston, pp 347–376

    Chapter  Google Scholar 

  • de Souza TC, de Castro EM, Magalhães PC, Lino LDO, Alves ET, de Albuquerque PEP (2013) Morphophysiology, morphoanatomy, and grain yield under field conditions for two maize hybrids with contrasting response to drought stress. Acta Physiol Plant 35:3201–3211

    Article  Google Scholar 

  • Epron D, Dreyer E (1992) Effects of severe dehydration on leaf photosynthesis in Quercus petruea (Matt.) Liebl: photosystem II efficiency, photochemical and nonphotochemical fluorescence quenching and electrolyte leakage. Tree Physiol 10:273–284

    Article  CAS  PubMed  Google Scholar 

  • Farquhar GD, Ehleringer JR, Hubick KT (1989) Carbon isotope discrimination and photosynthesis. Annu Rev Plant Biol 40:503–537

    Article  CAS  Google Scholar 

  • Flexas J, Medrano H (2002) Drought-inhibition of photosynthesis in C3 plants: stomatal and non-stomatal limitations revisited. Ann Bot 89:183–189

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hall AE, Richards RA, Condon AG, Wright GC, Farquhar GD (1994) Carbon isotope discrimination and plant breeding. In: Janick J (ed) Plant breeding reviews, vol 12. Wiley, New York, pp 81–113

    Google Scholar 

  • Halliwell B (1994) Free radicals and antioxidants: a personal view. Nutr Rev 52:253–265

    Article  CAS  PubMed  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–190

    Article  CAS  PubMed  Google Scholar 

  • Hogewoning SW, Wientjes E, Douwstra P, Trouwborst G, Van Ieperen W, Croce R, Harbinson J (2012) Photosynthetic quantum yield dynamics: from photosystems to leaves. Plant Cell 24:1921–1935

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Huang W, Yang SJ, Zhang SB, Zhang JL, Cao KF (2012) Cyclic electron flow plays an important role in photoprotection for the resurrection plant Paraboea rufescens under drought stress. Planta 235:819–828

    Article  CAS  PubMed  Google Scholar 

  • Kaiser WM (1987) Effects of water deficit on photosynthetic capacity. Physiol Plant 71:142–149

    Article  CAS  Google Scholar 

  • Kumar MS, Dahuja A, Rai RD, Walia S, Tyagi A (2014) Role of γ-oryzanol in drought-tolerant and susceptible cultivars of rice (Oryza sativa L.). Indian J Biochem Bio 51:75–80

    CAS  Google Scholar 

  • Latha M (2006) Germplasm characterization and evaluation of horticultural crops. Annu Rep NBPGR, New Delhi

    Google Scholar 

  • Lehtimaki N, Lintala M, Allahverdiyeva Y, Aro EM, Mulo P (2010) Drought stress-induced upregulation of components involved in ferredoxin-dependent cyclic electron transfer. J Plant Physiol 167:1018–1022

    Article  PubMed  Google Scholar 

  • Lerma-Garcia MJ, Herrero-Martinez JM, Simó-Alfonso EF, Mendonça CR, Ramis-Ramos G (2009) Composition, industrial processing and applications of rice bran γ-oryzanol. Food Chem 115:389–404

    Article  CAS  Google Scholar 

  • Loggini B, Scartazza A, Brugnoli E, Navari-Izzo F (1999) Antioxidative defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiol 119:1091–1100

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lopes MS, Reynolds MP (2010) Partitioning of assimilates to deeper roots is associated with cooler canopies and increased yield under drought in wheat. Funct Plant Biol 37:147–156

    Article  Google Scholar 

  • Misra G, Nandi S (2014) Enzymatic deacidification of rice bran oil containing high free fatty acids with recycling. Chem Sci Rev Lett 2:376–381

    Google Scholar 

  • Møller IM, Jensen PE, Hansson A (2007) Oxidative modifications to cellular components in plants. Annu Rev Plant Biol 58:459–481

    Article  PubMed  Google Scholar 

  • Murray JR, Hackett WP (1991) Dihydroflavonol reductase activity in relation to differential anthocyanin accumulation in juvenile and mature phase Hedera helix L. Plant Physiol 97:343–351

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nicolas ME, Lambers H, Simpson RJ, Dalling MJ (1985) Effect of drought on metabolism and partitioning of carbon in two wheat varieties differing in drought-tolerance. Annal Bot 55:727–742

    Article  CAS  Google Scholar 

  • Osakabe Y, Osakabe K, Shinozaki K, Tran LSP (2014) Response of plants to water stress. Front plant sci 5:86

    Article  PubMed  PubMed Central  Google Scholar 

  • Polívka T, Frank HA (2010) Molecular factors controlling photosynthetic light harvesting by carotenoids. Accounts chem Res 43:1125–1134

    Article  Google Scholar 

  • Pramachandra GS, Saneoka G, Ogata S (1990) Cell membrane stability, an indicator of drought tolerance, as affected by applied nitrogen in soyabean. J Agric Sci 115:63–66

    Article  Google Scholar 

  • Ranja S, Singh R, Singh M, Pathre UV, Shirke PA (2014) Characterizing photoinhibition and photosynthesis in juvenile-red versus mature-green leaves of Jatropha curcas L. Plant Physiol Biochem 79:48–59

    Article  Google Scholar 

  • Raymond CA, Harwood CE, Owen JV (1986) A conductivity method for screening populations of eucalypts for frost damage and frost tolerance. Aust J Bot 34:377–393

    Article  Google Scholar 

  • Roche Y, Gerbeau-Pissot P, Buhot B, Thomas D, Bonneau L, Gresti J, Mongrand S, Perrier-Cornet JM, Simon-Plas F (2008) Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts. FASEB J22:3980–3991

    Article  Google Scholar 

  • Rodrigues ML, Pacheco CMA, Chaves MM (1995) Soil-plant water relations, root distribution and biomass partitioning in Lupinus albus L. under drought conditions. J Exp Bot 46:947–956

    Article  CAS  Google Scholar 

  • Scartazza A, Lauteri M, Guido MC, Brugnoli E (1998) Carbon isotope discrimination in leaf and stem sugars, water-use efficiency and mesophyll conductance during different developmental stages in rice subjected to drought. Funct Plant Biol 25:489–498

    CAS  Google Scholar 

  • Sgherri C, Stevanovic B, Navari-Izzo F (2004) Role of phenolics in the antioxidative status of the resurrection plant Ramonda serbica during dehydration and rehydration. Physiol Plantarum 122:478–485

    Article  CAS  Google Scholar 

  • Shukla N, Awasthi RP, Rawat L, Kumar J (2012) Biochemical and physiological responses of rice (Oryza sativa L.) as influenced by Trichoderma harzianum under drought stress. Plant Physiol Biochem 54:78–88

    Article  CAS  PubMed  Google Scholar 

  • Singh R, Naskar J, Pathre UV, Shirke PA (2014) Reflectance and cyclic electron flow as an indicator of drought stress in cotton (Gossypium hirsutum). Photochem Photobiol 90:544–551

    Article  CAS  PubMed  Google Scholar 

  • Singh R, Pandey N, Naskar J, Shirke PA (2015) Physiological performance and differential expression profiling of genes associated with drought tolerance in contrasting varieties of two Gossypium species. Protoplasma 252:423–438

    Article  CAS  PubMed  Google Scholar 

  • Singh R, Pandey N, Kumar A, Shirke PA (2016) Physiological performance and differential expression profiling of genes associated with drought tolerance in root tissue of four contrasting varieties of two Gossypium species. Protoplasma 253:163–174

    Article  CAS  PubMed  Google Scholar 

  • Venuprasad R, Cruz MS, Amante M, Magbanua R, Kumar A, Atlin GN (2008) Response to two cycles of divergent selection for grain yield under drought stress in four rice breeding populations. Field Crop Res 107:232–244

    Article  Google Scholar 

  • Wellburn A (1994) The spectral determination of chlorophyll a and chlorophyll b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144:307–313

    Article  CAS  Google Scholar 

  • Wright GC, Rao RC, Farquhar GD (1994) Water-use efficiency and carbon isotope discrimination in peanut under water deficit conditions. Crop Sci 34:92–97

    Article  Google Scholar 

  • Yousfi S, Márquez AJ, Betti M, Araus JL, Serret MD (2016) Gene expression and physiological responses to salinity and water stress of contrasting durum wheat genotypes. J Integr Plant Biol 58:48–66

    Article  CAS  PubMed  Google Scholar 

  • Yu BP (1994) Cellular defenses against damage from reactive oxygen species. Physiol Rev 74:139–162

    CAS  PubMed  Google Scholar 

  • Zhang YL, Hu YY, Luo HH, Chow WS, Zhang WF (2011) Two distinct strategies of cotton and soybean differing in leaf movement to perform photosynthesis under drought in the field. Funct Plant Biol 38:567–575

    Article  CAS  Google Scholar 

  • Zhou Z, Robards K, Helliwell S, Blanchard C (2002) Ageing of stored rice: changes in chemical and physical attributes. J Cereal Sci 35:65–78

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Authors are thankful to The Director, CSIR-National Botanical Research Institute, Lucknow, for the facilities and for the financial support from the 12th Five Year Plan, Supra-Institutional project BSC-0204.

Authors’ contributions

All the experiments were performed by FK and PU. RS assisted in conceptualization guiding and supervising the experimental work, data collection and manuscript preparation. PKS assisted in conceptualization, guiding and supervising the experimental design and data collection. PAS was responsible for the overall conceptualization and supervision of the experiments, interpretation of the results and finalization of the manuscript.

Author information

Author notes

  1. Ruchi Singh

    Present address: Biotechnology Division, CSIR – Central Institute of Medicinal and Aromatic Plants, P.O-CIMAP, Near Kukrail Picnic Spot, Lucknow, 226 015, India

Authors and Affiliations

  1. Plant Physiology Division, Council of Scientific and Industrial Research (CSIR) - National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, India

    Furqan Khan, Priyanka Upreti, Ruchi Singh & Pramod Arvind Shirke

  2. Department of Biological Sciences, Sam Higginbotom Institute of Agriculture, Technology and Sciences, Naini, Allahabad, 211 007, India

    Pradeep Kumar Shukla

Authors
  1. Furqan Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Priyanka Upreti
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ruchi Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pradeep Kumar Shukla
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Pramod Arvind Shirke
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Pramod Arvind Shirke.

Ethics declarations

Conflict of interest

The authors wish to state that we have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Khan, F., Upreti, P., Singh, R. et al. Physiological performance of two contrasting rice varieties under water stress. Physiol Mol Biol Plants 23, 85–97 (2017). https://doi.org/10.1007/s12298-016-0399-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12298-016-0399-2

Keywords

Search

Navigation