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Optimizing micropropagation of drought resistant Pyrus boissieriana Buhse

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Abstract

The present study concentrated on introducing a micropropagation protocol for a drought resistant genotype from Pyrus boissieriana, which is the second most naturally widespread pear species in Iran with proper physiological and medicinal properties. Proliferating microshoot cultures were obtained by placing nodal segments on MS medium supplemented with BAP and IBA or NAA. The highest number of shoots (27 shoots per explant) were obtained with 1.5 mg l−1 BAP and 0.05 mg l−1 IBA, but this combination did not produce shoots of desirable length (>1.7 cm). Combination of 1.75 mg l−1 BAP and 0.07 mg l−1 IBA was the best for the shoot multiplication in P. boissieriana with a sufficient number of shoot production (22.33 shoots per explant) and relatively more appropriate shoot length. The larger and greenish leaves were obtained when PG was added to the best multiplication treatment. Microshoot elongation was carried out in 1/2 and 1/4 MS medium containing 50–100 mg l−1 PG with different concentrations of IBA or NAA at intervals of 30–60 days. Significant increase in shoot length was detected after 45–60 days of culture in the presence of PG. The highest shoot length (8 cm) was recorded on 1/2 MS medium supplemented with 0.5 mg l−1 IBA and 100 mg l−1 PG. GA3 negatively affected number and length of shoots and generally caused generation of red leaves. The highest percentage of root induction (100%) and root length (9 cm) were obtained on 1/6 strength MS medium supplemented with 0.005 mg l−1 IBA. All plantlets were hardened when transferred to ex vitro conditions through a period of 25–30 days. The results suggest axillary shoot proliferation of P. boissieriana could successfully be employed for propagation of candidate drought resistant seedling.

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Abbreviations

BAP:

6-Benzylaminopurine

GA3 :

Gibberellic acid

IAA:

Indole-3-acetic acid

IBA:

3-Indolyl-butyric acid

KIN:

Kinetin

MS:

Murashige and Skoog

NAA:

α-Naphthalene acetic acid

PG:

Phloroglucinol

PGRs:

Plant growth regulators

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Acknowledgements

We would like to thank Shahid Beheshti University for providing the financial and the instrumental supports that enabled us to carry out the project.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of New Technologies and Energy Engineering, Shahid Beheshti University, G. C., Tehran, Iran

    Maryam Zakavi, Hossein Askari & Neda Irvani

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  1. Maryam Zakavi
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  2. Hossein Askari
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  3. Neda Irvani
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Correspondence to Hossein Askari.

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Zakavi, M., Askari, H. & Irvani, N. Optimizing micropropagation of drought resistant Pyrus boissieriana Buhse. Physiol Mol Biol Plants 22, 583–593 (2016). https://doi.org/10.1007/s12298-016-0387-6

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