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In vitro direct shoot regeneration from proximal, middle and distal segment of Coleus forskohlii leaf explants

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Abstract

Coleus forskohlii is an endangered multipurpose medicinal plant that has widespread applications. In spite of this, there have been few attempts to propagate its cultivation in India. The present communication presents an in vitro rapid regeneration method using leaf explants of Coleus forskohlii through direct organogenesis. Leaf explants that were excised into three different segments i.e. proximal (P), middle (M) and distal (D) were cultured on Murashige and Skoog (MS) basal medium supplemented with cytokinins. MS Media containing 5.0 mg L−1 BAP (6-Benzylaminopurine) promoted regeneration of multiple shoots through direct organogenesis from the leaf, which were further elongated on MS media augmented with 0.1 mg L-1 BAP and 0.1 mg L−1 IAA (Indole-3-acetic acid), cytokinin and auxin combination. Regenerated and elongated shoots, when transferred to ose resulted in profuse rooting plants that were transferred to soil after acclimatization and maintained in a green house. The current protocol offers a direct, mass propagation method bypassing the callus phase of C. forskohlii and is suitable for conservation, large-scale commercial cultivation, and genetic transformation with agronomically desirable traits.

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Abbreviations

BAP:

6-benzylaminopurine

IAA:

indole-3-acetic acid

KIN:

kinetin

NAA:

α-naphthalene acetic acid

TDZ:

thidiazuron

References

  • Bhat SV, Bajwa BS, Dornauer H, De Souza and Fehlhaber HW (1977) Structure and stereochemistry of new labdane diterpenoid from Coleus forskohlii Briq. Tetrahedron Lett. 19: 1669–1672.

    Article  Google Scholar 

  • Biondi S and Thorpe TA (1982) Clonal propagation of forest tree species. In: Rao AN (Ed.) 1982, Proceedings Costed Symposium on Tissue Culture of Economically Important Plants. Forest Res Inst Malaysia pp 197–204.

  • Briggs BA, McCulloch SM and Edick LA (1988) Micropropagation of azaleas using thidiazuron. Acta Hort. 226: 205–208.

    Google Scholar 

  • Dubey MP, Srimal RC, Nityanand S and Dhawan BN (1981) Pharmacological studies on coleonol, ahypotensive diterpene from Coleus forskohlii. J. Ethnopharmacol. 3: 1–13.

    Article  CAS  PubMed  Google Scholar 

  • Farnum P, Timmis R and Kulp JL (1983). Biotechnology of forest yield. Sci. 219: 694–702.

    Article  CAS  Google Scholar 

  • Ibrahim KM, Collins JC and Collin HA (1992) Characterization of progeny of Coleus blumei following an in vitro selection for salt tolerance. Plant Cell Tiss. Org. Cult. 28: 139–145.

    Article  CAS  Google Scholar 

  • Luis PBC, Adriane CMGM, Silvica BRCC and anna Christina MB (1999) Plant regeneration from seedling explants of Eucalyptus grandis X E. urophylla. Plant Cell Tiss. Org. Cult. 56: 17–23.

    Article  Google Scholar 

  • Mao AA, Wetten A, Fay M and Caligari PDS (1995) In vitro propagation of Clerodendrum colebrookianum Walp., a potential natural antihypertensive medicinal plant. Plant Cell Rep. 14: 493–496

    Article  CAS  Google Scholar 

  • Minocha SC (1987) Plant growth regulators and morphogenesis in cell and tissue culture of forest trees. In: Bonga JM & Durjan DJ (eds) Cell and Tisssue Culture in Forestry, Vol. I. Martinus Nijhoff Publ., Dordrecht. pp 50–66.

    Google Scholar 

  • Mok DWS and Mok MC (2001) Cytokinin metabolism and action. Annu. Rev. Plant Physiol. Plant Mol. Biol. 52: 89–118.

    Article  CAS  PubMed  Google Scholar 

  • Monier C and Ochatt SJ (1995) Establishing micropropagation conditions for five Cotoneaster genotypes. Plant Cell Tiss. Org. Cult. 42: 275–281.

    Article  CAS  Google Scholar 

  • Prakash E, Sha Valli Khan PS, Reddy PS and Rao KR (1999) Regeneration of plants from seed-derived callus of Hybanthus enneaspermus L. Muell., a rare ethnobotanical herb. Plant Cell Rep. 18: 873–878.

    Article  CAS  Google Scholar 

  • Ponsamuel J, Samson NP and Anderson KP (1994) In vitro clonal propagation of country potato — an underexploited tuber plant. In Vitro 30: A71.

    Google Scholar 

  • Rajasri B and Sabita B (2001) In vitro multiplication of Coleus forskohlii Briq.: an approach towards shortening the protocol. In Vitro Cell. Dev. Biol.-plant 37: 572–575.

    Google Scholar 

  • Reddy PS, Rodrigues R and Rajasekharan R (2001) Shoot organogenesis and mass propagation of Coleus forskohlii from leaf derived callus. Plant Cell Tiss. Org. Cult. 66: 183–188.

    Article  CAS  Google Scholar 

  • Rupp RH, De Souza NJ and Dohadwalla AN (eds) (1986) Proceedings of the International Symposium on Forskolin, Bombay. January 1985 pp 28–29.

  • Seamon KB and Daly JW (1981) Forskolin: A unique diterpene activator of cAMP-generating systems. J. Cyclic Nucl. Res. 7: 201–24.

    CAS  Google Scholar 

  • Sen J and Sharma AK (1991) In vitro propagation of Coleus forskohlii Briq. for forskolin synthesis. Plant Cell Rep. 9: 696–698.

    Article  CAS  Google Scholar 

  • Sen J, Sharma AK, Sahu NP and Mahato SB (1992) Production of forskolin in in vitro cultures of Coleus forskohlii. Planta Med. 58: 324–327.

    Article  CAS  PubMed  Google Scholar 

  • Sharma N, Chandel KPS and Srivastava VK (1991) In vitro propagation of Coleus forskohlii Briq., a threatened medicinal plant. Plant Cell Rep. 10: 67–70.

    Article  CAS  Google Scholar 

  • Srinivasan M, Nachiappan V and Rajasekharan R (2006) Potential application of urea-derived herbicides as cytokinins in plant tissue culture. J. Biosci. 31: 599–605.

    Article  CAS  PubMed  Google Scholar 

  • Shrivastava S and Banerjee M (2008). In vitro clonal propagation of physic nut (Jatropha curcas L.): Influence of additives. Int. J. Integrative Biol. 3: 73–79.

    CAS  Google Scholar 

  • Wldi E, Schaffner W and Berger KB (1998) In vitro propagation of Petasites hybridus (Asteraceae) from leaf and petiole explants and from inflorescence buds. Plant Cell Rep. 18: 336–340.

    Article  Google Scholar 

  • Zagrajski N, Leljak-Levani Organogenesis and callogenesis in nodal, internodal and leaf explants of Coleus blumei Benth. Periodicum Biol. 99: 67–76.

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Authors and Affiliations

  1. College of Biotechnology & Allied Sciences, Allahabad Agricultural Institute Deemed University, Allahabad, 211 007, UP, India

    Gaurav Krishna & P. W. Ramteke

  2. Biotechnology Division, JK Agri Genetics Ltd., 1-10-177, 4th Floor, Varun Towers, Begumpet, Hyderabad, 500 016, AP, India

    Gaurav Krishna, P. Sairam Reddy & P. S. Bhattacharya

  3. Department of Biological Sciences, Purdue University, Indiana, 47906, USA

    N. Anoop Nair

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  1. Gaurav Krishna
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  2. P. Sairam Reddy
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  3. N. Anoop Nair
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  4. P. W. Ramteke
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  5. P. S. Bhattacharya
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Correspondence to Gaurav Krishna.

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Krishna, G., Sairam Reddy, P., Anoop Nair, N. et al. In vitro direct shoot regeneration from proximal, middle and distal segment of Coleus forskohlii leaf explants. Physiol Mol Biol Plants 16, 195–200 (2010). https://doi.org/10.1007/s12298-010-0021-y

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  • DOI: https://doi.org/10.1007/s12298-010-0021-y

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