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Prevalence and Viral Load of Human Parvovirus B19 (B19V) Among Blood Donors in South-East Brazil

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Abstract

The infection of human parvovirus B19 (B19V) is a common event in the general population, including volunteer blood donors. In some cases it can be asymptomatic and can remain persistent for a long period of time. The objective of this study was to examine the B19V DNA prevalence and viral load in first-time volunteer blood donors. Blood samples were collected from 91 primary blood donors at the Regional Blood Center of Ribeirão Preto, Southeast Brazil. Viral detection and quantitation was performed by an in-house TaqMan® real-time PCR with high sensitivity. B19V DNA was detected in one male blood donor (1.0 %) and was characterized by a very low viral load (537.36 copies/mL). Our studies demonstrate that B19V DNA at low titer may be present in apparently healthy individuals. Sensitive molecular diagnostic tools can be applied for the screening of fresh blood derived products in order to prevent transfusion-transmitted B19V infection.

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Acknowledgements

The study was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP, Brazil (Grants No. 2009/16623-1, CTC-1998/14.247-6 and INCTC-2008/57.877-3), and the Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (INCTC-573.754/2008-0).

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Authors and Affiliations

  1. Laboratory of Molecular Biology, Faculty of Medicine of Ribeirão Preto, Regional Blood Center of Ribeirão Preto, University of São Paulo, Rua Tenente Catão Roxo 2501, Ribeirão Preto, São Paulo, 14051-140, Brazil

    Svetoslav Nanev Slavov, Katia Kaori Otaguiri, Dimas Tadeu Covas & Simone Kashima

  2. Department of Clinical Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

    Svetoslav Nanev Slavov & Dimas Tadeu Covas

  3. Department of Clinical, Toxicological and Bromatological Analyses, School of Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto, Brazil

    Katia Kaori Otaguiri & Simone Kashima

Authors
  1. Svetoslav Nanev Slavov
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  2. Katia Kaori Otaguiri
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  3. Dimas Tadeu Covas
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  4. Simone Kashima
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Correspondence to Svetoslav Nanev Slavov.

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Slavov, S.N., Otaguiri, K.K., Covas, D.T. et al. Prevalence and Viral Load of Human Parvovirus B19 (B19V) Among Blood Donors in South-East Brazil. Indian J Hematol Blood Transfus 32 (Suppl 1), 323–325 (2016). https://doi.org/10.1007/s12288-015-0607-1

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  • DOI: https://doi.org/10.1007/s12288-015-0607-1

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