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Differentiation of Bone Marrow: Derived Mesenchymal Stem Cells into Hepatocyte-like Cells

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Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

Cirrhosis is the end-stage liver fibrosis, whereby normal liver architecture is disrupted by fibrotic bands, parenchymal nodules and vascular distortion. Portal hypertension and hepatocyte dysfunction are the end results and give rise to major systemic complications and premature death. Mesenchymal stem cells (MSC) have the capacity of self-renew and to give rise to cells of various lineages, so MSC can be isolated from bone marrow (BM) and induced to differentiate into hepatocyte-like cells. MSC were induced to differentiate into hepatocyte-like cells by hepatotic growth factor (HGF) and fibroblast growth factor-4 (FGF-4). Differentiated cells were examined for the expression of hepatocyte-specific markers and hepatocyte functions. MSC were isolated. Flow cytometry analysis showed that they expressed the MSC-specific markers, reverse transcriptase–polymerase chain reaction (RT-PCR) demonstrated that MSC expressed the hepatocyte-specific marker cytokeratin 18 (CK-18) following hepatocyte induction. This study demonstrates that BM-derived—MSC can differentiate into functional hepatocyte-like cells following the induction of HGF and FGF-4. MSC can serve as a favorable cell source for tissue engineering in the treatment of liver disease.

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Authors and Affiliations

  1. Clinical Pathology Department, Faculty of Medicine, Al kaser Al Aini, Cairo University, Cairo, Egypt

    Nesrien M. Al Ghrbawy, Reham Abdel Aleem Mohamed Afify & Nehal Dyaa

  2. Nasser Institute, Cairo, Egypt

    Asmaa A. El Sayed

Authors
  1. Nesrien M. Al Ghrbawy
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  2. Reham Abdel Aleem Mohamed Afify
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  3. Nehal Dyaa
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  4. Asmaa A. El Sayed
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Correspondence to Reham Abdel Aleem Mohamed Afify.

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Al Ghrbawy, N.M., Afify, R.A.A.M., Dyaa, N. et al. Differentiation of Bone Marrow: Derived Mesenchymal Stem Cells into Hepatocyte-like Cells. Indian J Hematol Blood Transfus 32, 276–283 (2016). https://doi.org/10.1007/s12288-015-0581-7

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  • DOI: https://doi.org/10.1007/s12288-015-0581-7

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