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Paenibacillus nuruki sp. nov., isolated from Nuruk, a Korean fermentation starter

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

A Gram-stain-positive, rod-shaped, non-endospore-forming motile by means of peritrichous flagella, facultatively anaerobic bacterium designated TI45-13arT was isolated from Nuruk, a Korean traditional Makgeolli fermentation starter. It grew at 4–35°C (optimum, 28–30°C), pH 5.0–9.0 (optimum, pH 7.0) and NaCl concentrations up to 5% (w/v). Phylogenetic trees generated using 16S rRNA gene sequences revealed that strain TI45-13arT belonged to the genus Paenibacillus and showed the highest sequence similarities with Paenibacillus kyungheensis DCY88T (98.5%), Paenibacillus hordei RH-N24T (98.4%) and Paenibacillus nicotianae YIM h-19T (98.1%). The major fatty acid was anteiso-C15:0. The DNA G+C content was 39.0 mol%, and MK-7 was the predominant isoprenoid quinone. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified glycolipids, and one unidentified aminoglycolipid. The cell-wall peptidoglycan contained meso-diaminopimelic acid. On the basis of polyphasic taxonomy study, it was suggested that strain TI45-13arT represents a novel species within the genus Paenibacillus for which the name Paenibacillus nuruki sp. nov. is proposed. The type strain was TI45-13arT (= KACC 18728T = NBRC 112013T).

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Acknowledgements

This study was carried out with the support (Project no. PJ-011248) of the National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, Republic of Korea

    Soo-Jin Kim, Hayoung Cho, Jae-Hyung Ahn, Hang-Yeon Weon, Jeong-Seon Kim & Soon-Wo Kwon

  2. Rural Development Administration, National Institute of Horticultural and Herbal Science, Jeju, 63240, Republic of Korea

    Jae-Ho Joa

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  1. Soo-Jin Kim
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  3. Jae-Hyung Ahn
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Correspondence to Soon-Wo Kwon.

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The authors declare that there are no conflicts of interest.

Supplemental material for this article may be found at http://www.springerlink.com/content/120956.

The GenBank number for the 16S rRNA gene sequence and the genome sequence of strain TI45-13arT are KY419705 and MDER00000000, respectively.

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Kim, SJ., Cho, H., Ahn, JH. et al. Paenibacillus nuruki sp. nov., isolated from Nuruk, a Korean fermentation starter. J Microbiol. 57, 836–841 (2019). https://doi.org/10.1007/s12275-019-9118-3

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  • DOI: https://doi.org/10.1007/s12275-019-9118-3

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