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Pyrosequencing-based analysis of fecal microbial communities in three purebred pig lines

  • Microbial Ecology and Environmental Microbiology
  • Published:
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Abstract

This study examined the fecal bacterial diversity of 15-weekold pigs from three purebred lines: Duroc, Landrace, and Yorkshire. Taxon-dependent and -independent analyses were performed to evaluate differences in the fecal bacterial communities and to identify bacterial genera that can be used to discriminate breeds, following high-throughput pyrosequencing of 16S rRNA genes. Among the breeds evaluated, Landrace had the most diverse bacterial community composition. Prevotella, Blautia, Oscillibacter, and Clostridium were detected in all samples regardless of breed. On the other hand, Catenibacterium, Blautia, Dialister, and Sphaerochaeta were differentially detected among breeds, as demonstrated by the canonical loading plot. The discriminant analysis of principal components plot also showed clear separation of the three purebred pig lines, with a certain degree of similarity between Landrace and Yorkshire pigs and a distinct separation between Duroc pigs and the other two breeds. Other factors not related to breed, such as season or time of sampling and pen effects, may contribute to shaping the gut microbiota of pigs.

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Authors and Affiliations

  1. Department of Animal Resources Science, Dankook University, Cheonan, 330-714, Republic of Korea

    Edward Alain B. Pajarillo, Jong Pyo Chae, Marilen P. Balolong, Hyeun Bum Kim & Dae-Kyung Kang

  2. Department of Biology, University of the Philippines Manila, Manila, 1000, Philippines

    Marilen P. Balolong

  3. Department of Animal Science and Technology, Sunchon National University, Sunchon, 540-950, Republic of Korea

    Kang-Seok Seo

Authors
  1. Edward Alain B. Pajarillo
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  2. Jong Pyo Chae
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  3. Marilen P. Balolong
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  4. Hyeun Bum Kim
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  5. Kang-Seok Seo
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Correspondence to Dae-Kyung Kang.

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These authors contributed equally to this work.

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Pajarillo, E.A.B., Chae, J.P., Balolong, M.P. et al. Pyrosequencing-based analysis of fecal microbial communities in three purebred pig lines. J Microbiol. 52, 646–651 (2014). https://doi.org/10.1007/s12275-014-4270-2

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  • DOI: https://doi.org/10.1007/s12275-014-4270-2

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