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Alicyclobacillus tengchongensis sp. nov., a thermo-acidophilic bacterium isolated from hot spring soil

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Abstract

A thermo-acidophilic bacterium, designated strain ACK006T, was isolated from the soil of a hot spring at Tengchong in China. Cells were Gram-staining-positive, motile, catalase-positive and oxidase-negative, spore-forming rods. The isolate grew aerobically at 30–50°C (optimum at 45°C), pH 2.0–6.0 (optimum pH 3.2) and 0–5.0% (w/v) NaCl (optimum 1% NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain ACK006T belongs to the genus Alicyclobacillus with the sequence similarity of 92.3, 92.4, 92.5, and 92.8% to Alicyclobacillus cycloheptanicus SCHT, Alicyclobacillus ferrooxydans TC-34T, Alicyclobacillus contaminans 3-A191T and Alicyclobacillus disulfidooxidans SD-11T, respectively. Similarity to other species of the genus Alicyclobacillus was 90.3–92.8% and similarity to species of the genus Tumebacillus was 85.9–87.8%. The genomic DNA G+C content was 53.7 mol%. The predominant menaquinone was MK-7. Major fatty acids were ω-cycloheptane C18:0, iso-C17:0 and anteiso-C17:0. The cell-wall peptidoglycan was the A1γ type; containing meso-diaminopimelic acid as the diagnostic diamino acid. On the basis of polyphasic analysis from this study, strain ACK006T represents a novel species of the genus Alicyclobacillus for which the name Alicyclobacillus tengchongensis sp. nov. is proposed. The type strain is ACK006T (=KCTC 33022T =DSM 25924T).

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Authors and Affiliations

  1. Department of Biomolecular Science, University of Science and Technology, Daejeon, 305-350, Republic of Korea

    Min Goo Kim & Chang-Jin Kim

  2. Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea

    Min Goo Kim, Dong-Jin Park & Chang-Jin Kim

  3. Institute of Microbial Ecology and Resources, Mokwon University, Daejeon, 302-729, Republic of Korea

    Jae-Chan Lee

  4. The Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China

    Wen-Jun Li

  5. Key Laboratory of Biogeography and Bioresource in Arid Land, Chinese Academy of Science, Xinjiang Institute of Ecology and Geography, Chinese Acedemy of Sciences, Ürümqi, Xinjiang, 830011, P. R. China

    Wen-Jun Li

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  1. Min Goo Kim
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  2. Jae-Chan Lee
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Correspondence to Chang-Jin Kim.

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Supplemental material for this article may be found at http://www.springerlink.com/content/120956.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of strain ACK006T is KF772795.

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Kim, M.G., Lee, JC., Park, DJ. et al. Alicyclobacillus tengchongensis sp. nov., a thermo-acidophilic bacterium isolated from hot spring soil. J Microbiol. 52, 884–889 (2014). https://doi.org/10.1007/s12275-014-3625-z

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  • DOI: https://doi.org/10.1007/s12275-014-3625-z

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