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Expression analysis of LeNHX1 gene in mycorrhizal tomato under salt stress

  • Microbial Genetics, Genomics and Molecular Biology
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Abstract

The plant growth, stem sap flow, Na+ and Cl− content, and the expression of vacuolar Na+/H+ antiporter gene (LeNHX1) in the leaves and roots of tomato under different NaCl stresses (0.5% and 1%) were studied to analyze the effect of arbuscular mycorrhizal fungi (AMF) on Na+ and Cl− accumulation and ion exchange. The results showed that arbuscular mycorrhizal (AM) plant growth and stem sap flow increased and salt tolerance improved, whereas Na+ and Cl− accumulated. Na+ significantly decreased, and no significant decline was detected in Cl− content after AMF inoculation compared with the non-AM plants. The LeNHX1 gene expression was induced in the AM and non-AM plants by NaCl stress. However, AMF did not improve the LeNHX1 level, and low expression was observed in the AM tomato. Hence, the mechanism that reduced the Na+ damage to tomato induced by AMF has little relation to LeNHX1, which can export Na+ from the cytosol to the vacuole across the tonoplast.

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Authors and Affiliations

  1. College of Horticulture, Sichuan Agricultural University, Yáan, Sichuan, 625014, P. R. China

    ZhongQun He & Zhi Huang

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  1. ZhongQun He
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  2. Zhi Huang
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Correspondence to ZhongQun He or Zhi Huang.

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These authors contributed equally to this work.

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He, Z., Huang, Z. Expression analysis of LeNHX1 gene in mycorrhizal tomato under salt stress. J Microbiol. 51, 100–104 (2013). https://doi.org/10.1007/s12275-013-2423-3

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  • DOI: https://doi.org/10.1007/s12275-013-2423-3

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