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Beginning to understand the role of sugar carriers in Colletotrichum lindemuthianum: the function of the gene mfs1

  • Microbial Genetics, Genomics and Molecular Biology
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Abstract

Fungi of the Colletotrichum genus are among the most prominent phytopathogens that cause diseases with a considerable economic impact, such as anthracnose. The hemibiotrophic fungus Colletotrichum lindemuthianum (teleomorph Glomerella cingulata f. sp. phaseoli) is the causal agent of the anthracnose of the common bean; and similarly to other phytopathogens, it uses multiple strategies to gain access to different carbon sources from its host. In this study, we examine mfs1, a newly identified C. lindemuthianum hexose transporter. The mfs1 gene is expressed only during the necrotrophic phase of the fungus’ interaction within the plant and allows it to utilize the available sugars during this phase. The deletion of mfs1 gene resulted in differential growth of the fungus in a medium that contained glucose, mannose or fructose as the only carbon source. This study is the first to describe a hexose transporter in the hemibiotrophic pathogen C. lindemuthianum and to demonstrate the central role of this protein in capturing carbon sources during the necrotrophic development of the plant/pathogen interaction.

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  1. Laboratório de Genética Molecular de Micro-organismos, BIOAGRO, Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, 36570-000, Minas Gerais, Brazil

    Monalessa Fábia Pereira, Carolina Maria de Araújo dos Santos, Elza Fernandes de Araújo, Marisa Vieira de Queiroz & Denise Mara Soares Bazzolli

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  1. Monalessa Fábia Pereira
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  2. Carolina Maria de Araújo dos Santos
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  3. Elza Fernandes de Araújo
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  4. Marisa Vieira de Queiroz
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  5. Denise Mara Soares Bazzolli
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Correspondence to Denise Mara Soares Bazzolli.

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Pereira, M.F., de Araújo dos Santos, C.M., de Araújo, E.F. et al. Beginning to understand the role of sugar carriers in Colletotrichum lindemuthianum: the function of the gene mfs1. J Microbiol. 51, 70–81 (2013). https://doi.org/10.1007/s12275-013-2393-5

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