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Winogradskyella jejuensis sp. nov., a marine bacterium isolated from a brown alga Carpopeltis affinis

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Abstract

A Gram-negative, orange-pigmented, rod-shaped bacterium, designated strain CP32T was isolated from a brown alga Carpopeltis affinis collected on the coast of Jeju Island, Republic of Korea. The isolate grew at 10–37°C (optimum 25°C) and at pH 6.5–9.5 (optimum pH 7.0). The 16S rRNA gene sequence of the isolate showed much similarity with the type strains of recognized species of the genus Winogradskyella (94.0–96.6%). The most closely related species were Winogradskyella echinorum KMM 6211T, Winogradskyella ulvae KMM 6390T, Winogradskyella thalassocola KMM 3907T, Winogradskyella poriferorum UST030701-295T, and Winogradskyella eximia KMM 3944T. The major respiratory quinone was menaquinone-6 (MK-6) and the predominant cellular fatty acids were iso-C15:1 G (24.8%), iso-C15:0 (23.4%), and iso-C17:0 3-OH (11.6 %). The DNA G+C content was 33.3 mol%. The polar lipid profile was composed of phosphatidylethanolamine, two aminolipids, and five unknown lipids. On the basis of phenotypic features, and the result of 16S rRNA gene sequence analysis, strain CP32T (=KCTC 23835T =JCM 18454T) represents a novel species of the genus Winogradskyella, for which the name Winogradskyella jejuensis sp. nov. is proposed.

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References

  • Bernardet, J.-F. 2011. Family I. Flavobacteriaceae Reichenbach 1992. pp. 106–111. Bergey’s Manual of Systematic Bacteriology, 2nd ed. In Whitman, W. (ed.), The Williams & Wilkins Co., Baltimore, USA.

    Google Scholar 

  • Bernardet, J.-F. and Nakagawa, Y. 2006. An introduction to the family Flavobacteriaceae. pp. 455–480. The Prokaryotes. A Handbook on the Biology of Bacteria, 3rd edn. In Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K.H., and Stackebrandt, E. (eds.) Springer, New York, N.Y., USA.

    Google Scholar 

  • Bernardet, J.-F., Nakagawa, Y., and Holmes, B. 2002. Proposal minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int. J. Syst. Evol. Microbiol. 52, 1049–1070.

    Article  PubMed  CAS  Google Scholar 

  • Chun, J., Lee, J.H., Jung, Y., Kim, M., Kim, S., Kim, B.K., and Lim, Y.W. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57, 2259–2261.

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein, J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. J. Mol. Evol. 17, 368–376.

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.

    Article  Google Scholar 

  • Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41, 95–98.

    CAS  Google Scholar 

  • Ivanova, E.P., Christen, R., Gorshkova, N.M., Zhukova, N.V., Kurilenko, V.V., Crawford, R.J., and Mikhailov, V.V. 2010. Winogradskyella exilis sp. nov., isolated from the starfish Stellaster equestris, and emended description of the genus Winogradskyella. Int. J. Syst. Evol. Microbiol. 60, 1577–1580.

    Article  PubMed  CAS  Google Scholar 

  • Kim, S.B. and Nedashkovskaya, O.I. 2010. Winogradskyella pacifica sp. nov., a marine bacterium of the family Flavobacteriaceae. Int. J. Syst. Evol. Microbiol. 60, 1948–1951.

    Article  PubMed  CAS  Google Scholar 

  • Kluge, A.G. and Farris, F.S. 1969. Quantitative phyletics and the evolution of anurans. Syst. Zool. 18, 1–32.

    Article  Google Scholar 

  • Komagata, K. and Suzuki, K. 1987. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol. 19, 161–207.

    Article  CAS  Google Scholar 

  • Lau, S.C.K., Tsoi, M.M.Y., Li, X., Plakhotnikova, I., Dobretsov, S., Lau, K.W.K., Wu, M., Wong, P.-K., Pawlik, J.R., and Qian, P.-Y. 2005. Winogradskyella poriferorum sp nov., a novel member of the family Flavobacteriaceae isolated from a sponge in the Bahamas. Int. J. Syst. Evol. Microbiol. 55, 1589–1592.

    Article  PubMed  CAS  Google Scholar 

  • Lee, S.Y., Park, S., Oh, T.K., and Yoon, J.H. 2012. Winogradskyella aquimaris sp. nav., isolated from seawater. Int. J. Syst. Evol. Microbiol. 62, 1814–1818.

    Article  PubMed  CAS  Google Scholar 

  • Marmur, J. 1961. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3, 208–218.

    Article  CAS  Google Scholar 

  • Marmur, J. and Doty, P. 1962. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5, 109–118.

    Article  PubMed  CAS  Google Scholar 

  • Minnikin, D.E., O’Donnell, A.G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A., and Parlett, J.H. 1984. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2, 233–241.

    Article  CAS  Google Scholar 

  • Nedashkovskaya, O.I., Kim, S.B., Han, S.K., Snauwaert, C., Vancanneyt, M., Swings, J., Kim, K.-O., Lysenko, A.M., Rohde, M., Frolova, G.M., and et al. 2005. Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae. Int. J. Syst. Evol. Microbiol. 55, 49–55.

    Article  PubMed  CAS  Google Scholar 

  • Nedashkovskaya, O.I., Kukhlevskiy, A.D., and Zhukova, N.V. 2012. Winogradskyella ulvae sp. nov., an epiphyte of a Pacific seaweed, and emended descriptions of the genus Winogradskyella and Winogradskyella thalassocola, Winogradskyella echinorum, Winogradskyella exilis and Winogradskyella eximia. Int. J. Syst. Evol. Microbiol. 62, 1450–1456.

    Article  PubMed  CAS  Google Scholar 

  • Nedashkovskaya, O.I., Vancanneyt, M., Kim, S.B., and Zhukova, N.V. 2009. Winogradskyella echinorum sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius. Int. J. Syst. Evol. Microbiol. 59, 1465–1468.

    Article  PubMed  CAS  Google Scholar 

  • Pinhassi, J., Nedashkovskaya, O.I., Hagström, A., and Vancanneyt, M. 2009. Winogradskyella rapida sp. nov., isolated from protein-enriched seawater. Int. J. Syst. Evol. Microbiol. 59, 2180–2184.

    Article  PubMed  CAS  Google Scholar 

  • Romanenko, L.A., Tanaka, N., Frolova, G.M., and Mikhailov, V.V. 2009. Winogradskyella arenosi sp. nov., a member of the family Flavobacteriaceae isolated from marine sediments from the Sea of Japan. Int. J. Syst. Evol. Microbiol. 59, 1443–1446.

    Article  PubMed  CAS  Google Scholar 

  • Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.

    PubMed  CAS  Google Scholar 

  • Sasser, M. 1990. Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101.: MIDI Inc., Newark, DE, USA.

    Google Scholar 

  • Smibert, R.M. and Krieg, N.R. 1994. Phenotypic characterization. Methods for General and Molecular Bacteriology. pp. 607–654. In Gerhardt, P., Murray, R.G.E., Wood, W.A., and Krieg, N.R. (eds.). American Society for Microbiology, Washington, D.C., USA.

    Google Scholar 

  • Stackebrandt, E. and Goebel, B.M. 1994. A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Evol. Microbiol. 44, 846–849.

    CAS  Google Scholar 

  • Tamaoka, J. and Komagata, K. 1984. Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25, 125–128.

    Article  CAS  Google Scholar 

  • Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28, 2731–2739.

    Article  PubMed  CAS  Google Scholar 

  • Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876–4882.

    Article  PubMed  CAS  Google Scholar 

  • Weisburg, W.G., Barns, S.M., Pelletier, D.A., and Lane, D.J. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173, 697–703.

    PubMed  CAS  Google Scholar 

  • Yoon, B.J., Byun, H.D., Kim, J.Y., Lee, D.H., Kahng, H.Y., and Oh, D.C. 2011. Winogradskyella lutea sp. nov., isolated from seawater, and emended description of the genus Winogradskyella. Int. J. Syst. Evol. Microbiol. 61, 1539–1543.

    Article  PubMed  CAS  Google Scholar 

  • ZoBell, C.E. 1941. Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J. Mar. Res. 4, 42–75.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Jeju National University, Jeju, 690-756, Republic of Korea

    Ji-Young Kim & Duck-Chul Oh

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  1. Ji-Young Kim
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  2. Duck-Chul Oh
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Correspondence to Ji-Young Kim.

Additional information

The GenBank/EMBL/DDBJ accession number of the 16S rRNA gene sequence of strain CP32T is JF820844.

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Kim, JY., Oh, DC. Winogradskyella jejuensis sp. nov., a marine bacterium isolated from a brown alga Carpopeltis affinis . J Microbiol. 50, 888–892 (2012). https://doi.org/10.1007/s12275-012-2447-0

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  • DOI: https://doi.org/10.1007/s12275-012-2447-0

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