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Antifungal activity of extracellular hydrolases produced by autolysing Aspergillus nidulans cultures

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Abstract

Carbon-starving Aspergillus nidulans cultures produce high activities of versatile hydrolytic enzymes and, among these, ChiB endochitinase and EngA β-1,3-endoglucanase showed significant antifungal activity against various fungal species. Double deletion of engA and chiB diminished the antifungal activity of the fermentation broths and increased conidiogenesis and long-term viability of A. nidulans, but decreased the growth rate on culture media containing weak carbon sources. Production of ChiB and EngA can influence fungal communities either directly due to their antifungal properties or indirectly through their effects on vegetative growth. Our data suggest saprophytic fungi as promising future candidates to develop novel biocontrol technologies.

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References

  • Adams, T.H., Wieser, J.K., and Yu, J.H. 1998. Asexual sporulation in Aspergillus nidulans. Microbiol. Mol. Biol. Rev. 62, 35–54.

    PubMed  CAS  Google Scholar 

  • Bainbridge, B.W., Bull, A.T., Pirt, S.J., Rowley, B.I., and Trinci, A.P.J. 1971. Biochemical and structural changes in non-growing maintained and autolysing cultures of Aspergillus nidulans. Trans. Br. Mycol. Soc. 56, 371–385.

    Article  CAS  Google Scholar 

  • Barratt, R.W., Johnson, G.B., and Ogata, W.N. 1965. Wild-type and mutant stocks of Aspergillus nidulans. Genetics 52, 233–246.

    PubMed  CAS  Google Scholar 

  • Benitez, T., Rincon, A.M., Limon, M.C., and Codon, A.C. 2004. Biocontrol mechanisms of Trichoderma strains. Int. Microbiol. 7, 249–260.

    PubMed  CAS  Google Scholar 

  • De Marco, J.L. and Felix, C.R. 2002. Characterization of a protease produced by a Trichoderma harzianum isolate which controls cocoa plant witches’ broom disease. BMC Biochem. 3, 3.

    Article  PubMed  Google Scholar 

  • Elad, Y. 2000. Biological control of foliar pathogens by means of Trichoderma harzianum and potential modes of action. Crop Prot. 19, 709–714.

    Article  Google Scholar 

  • El-Katatny, M.H., Gudelj, M., Robra, K.H., Elnaghy, M.A., and Gübitz, G.M. 2001. Characterization of a chitinase and an endobeta-1,3-glucanase from Trichoderma harzianum Rifai T24 involved in control of the phytopathogen Sclerotium rolfsii. Appl. Microbiol. Biotechnol. 56, 137–143.

    Article  PubMed  CAS  Google Scholar 

  • Emri, T., Molnár, Zs., Szilágyi, M., and Pócsi, I. 2008. Regulation of autolysis in Aspergillus nidulans. Appl. Biochem. Biotechnol. 151, 211–220.

    Article  PubMed  CAS  Google Scholar 

  • Emri, T., Szilágyi, M., László, K., Hamvas, M., and Pócsi, I. 2009. PepJ is a new extracellular protease of Aspergillus nidulans. Folia Microbiol. 54, 105–109.

    Article  CAS  Google Scholar 

  • Erdei, E., Pusztahelyi, T., Miskei, M., Barna, T., and Pócsi, I. 2008. Characterization and heterologous expression of an age-dependent fungal/bacterial type chitinase of Aspergillus nidulans. Acta Microbiol. Immunol. Hung. 55, 351–361.

    Article  PubMed  CAS  Google Scholar 

  • Fravel, D., Olivain, C., and Alabouvette, C. 2003. Fusarium oxysporum and its biocontrol. New Phytol. 157, 493–502.

    Article  Google Scholar 

  • Gohel, V., Singh, A., Vimal, M., Ashwini, P., and Chhatpar, H.S. 2006. Bioprospecting and antifungal potential of chitinolytic microorganisms. Afr. J. Biotechnol. 5, 54–72.

    Google Scholar 

  • Haas, D. and Défago, G. 2005. Biological control of soil-borne pathogens by fluorescent pseudomonads. Nat. Rev. Microbiol. 3, 307–319.

    Article  PubMed  CAS  Google Scholar 

  • Harman, G.E., Howell, C.R., Viterbo, A., Chet, I., and Lorito, M. 2004. Trichoderma species: opportunistic, avirulent plant symbionts. Nature Rev. Microbiol. 2, 43–56.

    Article  CAS  Google Scholar 

  • Hong, T.Y. and Meng, M. 2003. Biochemical characterization and antifungal activity of an endo-1,3-beta-glucanase of Paenibacillus sp. isolated from garden soil. Appl. Microbiol. Biotechnol. 61, 472–478.

    PubMed  CAS  Google Scholar 

  • Karasuda, S., Tanaka, S., Kajihara, H., Yamamoto, Y., and Koga, D. 2003. Plant chitinase as a possible biocontrol agent for use instead of chemical fungicides. Biosci. Biotechnol. Biochem. 67, 221–224.

    Article  PubMed  CAS  Google Scholar 

  • Kim, Y., Islam, N., Moss, B.J., Nandakumar, M.P., and Marten, M.R. 2011. Autophagy induced by rapamycin and carbon-starvation have distinct proteome profiles in Aspergillus nidulans. Biotechnol. Bioeng. 108, 2705–2715.

    Article  PubMed  CAS  Google Scholar 

  • Liu, B., Lu, Y., Xin, Z., and Zhang, Z. 2009. Identification and antifungal assay of a wheat beta-1,3-glucanase. Biotechnol. Lett. 31, 1005–1010.

    Article  PubMed  CAS  Google Scholar 

  • Markovich, N.A. and Kononova, G.L. 2003. Lytic enzymes of Trichoderma and their role in plant defense from fungal diseases: A review. Appl. Biochem. Microbiol. 39, 341–351.

    Article  CAS  Google Scholar 

  • McIntyre, M., Berry, D.R., and McNeil, B. 1999. Response of Penicillium chrysogenum to oxygen starvation in glucose and nitrogen limited chemostat cultures. Enzyme Microb. Technol. 25, 447–454.

    Article  CAS  Google Scholar 

  • McIntyre, M., Berry, D.R., and McNeil, B. 2000. Role of proteases in autolysis of Penicillium chrysogenum chemostat cultures in response to nutrient depletion. Appl. Microbiol. Biotechnol. 53, 235–242.

    Article  PubMed  CAS  Google Scholar 

  • Molnár, Zs., Emri, T., Zavaczki, E., Pusztehelyi, T., and Pócsi, I. 2006. Effects of mutations in the GanB/RgsA G protein mediated signaling on the autolysis of Aspergillus nidulans. J. Basic Microbiol. 46, 495–603.

    Article  PubMed  Google Scholar 

  • Nielsen, P. and Sorensen, J. 1997. Multi-target and medium-independent fungal antagonism by hydrolytic enzymes in Paenibacillus polymyxa and Bacillus pumilus strains from barley rhizosphere. FEMS Microbiol. Ecol. 22, 183–192.

    Article  CAS  Google Scholar 

  • Pócsi, I., Leiter, É., Kwon, N.J., Shin, K.S., Kwon, G.S., Pusztahelyi, T., Emri, T., Abuknesha, R.A., Price, R.G., and Yu, J.H. 2009. Asexual sporulation signaling regulates autolysis of Aspergillus nidulans via modulating the chitinase ChiB production. J. Appl. Microbiol. 107, 514–523.

    Article  PubMed  Google Scholar 

  • Saiprasad, G.V.S., Mythili, J.B., Lalitha, A., Suneetha, C., Rashimi, H.J., Naveena, C., and Girija, G. 2009. Development of Trichoderma harzianum endochitinase gene construct conferring antifungal activity in transgenic tobacco. Indian J. Biotechnol. 8, 199–206.

    CAS  Google Scholar 

  • Schuster, A. and Schmoll, M. 2010. Biology and biotechnology of Trichoderma. Appl. Microbiol. Biotechnol. 87, 787–799.

    Article  PubMed  CAS  Google Scholar 

  • Sharma, N., Sharma, K.P., Gaur, R.K., and Gupta, V.K. 2011. Role of chitinase in plant defense. Asian J. Biochem. 6, 29–37.

    Article  CAS  Google Scholar 

  • Shin, K.S., Kwon, N.J., Kim, Y.H., Park, H.S., Kwon, G.S., and Yu, J.H. 2009. Differential roles of the ChiB chitinase in autolysis and cell death of Aspergillus nidulans. Eukaryot. Cell. 8, 738–746.

    Article  PubMed  CAS  Google Scholar 

  • Szilágyi, M., Kwon, N.J., Bakti, F., M-Hamvas, M., Jámbrik, K., Park, H.S., Pócsi, I., Yu, J.H., and Emri, T. 2011. Extracellular proteinase formation in carbon starving Aspergillus nidulans cultures — physiological function and regulation. J. Basic Microbiol. 51, 625–634.

    Article  PubMed  Google Scholar 

  • Szilágyi, M., Kwon, N.J., Dorogi, Cs., Pócsi, I., Yu, J.H., and Emri, T. 2010. The extracellular β-1,3-endoglucanase EngA is involved in autolysis of Aspergillus nidulans. J. Appl. Microbiol. 109, 1498–1508.

    PubMed  Google Scholar 

  • Yu, J.H. 2010. Regulation of development in Aspergillus nidulans and Aspergillus fumigatus. Mycobiology 38, 229–237.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary

    Melinda Szilágyi, Fruzsina Anton, Katalin Forgács, István Pócsi & Tamás Emri

  2. Departments of Bacteriology and Genetics, University of Wisconsin, Madison, WI, USA

    Jae-Hyuk Yu

Authors
  1. Melinda Szilágyi
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  2. Fruzsina Anton
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  3. Katalin Forgács
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  4. Jae-Hyuk Yu
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  5. István Pócsi
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  6. Tamás Emri
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Corresponding author

Correspondence to Tamás Emri.

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Szilágyi, M., Anton, F., Forgács, K. et al. Antifungal activity of extracellular hydrolases produced by autolysing Aspergillus nidulans cultures. J Microbiol. 50, 849–854 (2012). https://doi.org/10.1007/s12275-012-2001-0

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  • DOI: https://doi.org/10.1007/s12275-012-2001-0

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