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Inhibitory effects of sesamin on human osteoclastogenesis

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Abstract

The promotional nature of sesamin on human osteoblast differentiation has been proven. Here, the effect of sesamin on human osteoclasts was investigated in terms of differentiation and function in M-CSF and RANKL induced human PBMCs. Sesamin treatment significantly decreased the number of differentiated osteoclasts observed by TRAP staining; however, sesamin inhibition did not result from the alteration of precursor cell proliferation. Sesamin did not decrease NFATc1 gene expression, which opposed the decreasing trend of CathK and TRAP expression. DC-STAMP, but not Atp6v0d2, also significantly decreased in the presence of 14 µM sesamin. Expressions of CCR2b and CCR4 as chemokine receptors were significantly down-regulated. Sesamin might mediate the inhibition of human osteoclast differentiation, the recruitment of precursor cells and F-actin formation. Decrease in the area of the resorption pits and the collagen released from the bone slices under sesamin treatment emphasized the inhibitory effects on both the differentiation and function of osteoclasts. Sesamin is a promising phytochemical agent inhibiting osteoclast differentiation and function.

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Acknowledgements

We would like to thank Assistant Professor Supanchart Chayarop of the Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Chiang Mai University for the technical training of osteoclast differentiation and for the generous gift of Phalloidin-conjugated Alexa Flour 488. This research was supported by a grant under the program Strategic Scholarship for Frontier Research Network for Ph.D., Thai Doctoral Degree, Office of the Higher Education Commission and Thailand Excellent Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University.

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  1. Department of Biochemistry, Faculty of Medicine, Thailand Excellence Center for Tissue Engineering and Stem Cells, Chiang Mai University, Chiang Mai, 50200, Thailand

    Orawan Wanachewin, Peraphan Pothacharoen, Prachya Kongtawelert & Thanyaluck Phitak

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  1. Orawan Wanachewin
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  2. Peraphan Pothacharoen
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  3. Prachya Kongtawelert
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  4. Thanyaluck Phitak
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Correspondence to Thanyaluck Phitak.

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Wanachewin, O., Pothacharoen, P., Kongtawelert, P. et al. Inhibitory effects of sesamin on human osteoclastogenesis. Arch. Pharm. Res. 40, 1186–1196 (2017). https://doi.org/10.1007/s12272-017-0926-x

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  • DOI: https://doi.org/10.1007/s12272-017-0926-x

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