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Expression system for production of bioactive compounds, recombinant human adiponectin, in the silk glands of transgenic silkworms

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Abstract

Adiponectin is an adipocyte hormone involved in glucose and lipid metabolism. The aim of this study was to develop a human adiponectin expression system in transgenic silkworm using a human adiponectin expression vector. The silk gland of the silkworm is a highly specialized organ that has the wonderful ability to synthesize and secrete silk protein. To express human adiponectin in the silk gland of transgenic silkworm, targeting vectors pB-A3-adiponectin-IRES-RFP and pB-Ser1-adiponectin-IRES-RFP were constructed and then introduced into the silkworm pupa. The transgenic silkworms were verified by PCR and then generated. The level of adiponectin in the transgenic silkworm was 6–10 ng/50 mg of freeze-dried powder, and western blotting using an antibody against human adiponectin demonstrated a specific band with a molecular weight of 30 kDa in the silkworm. These results showed that human adiponectin introduced into the silkworm genome was expressed successfully on a large-scale.

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Acknowledgments

This paper was supported by the Sahmyook University Research fund.

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Authors and Affiliations

  1. College of Pharmacy, Sahmyook University, Hwarangro-815, Nowon-gu, Seoul, 139-742, Korea

    Seulmee Shin, Aeri Lee, Sungwon Lee, Hyunseok Kong, Youngcheon Song & Kyungjae Kim

  2. B2Bio Co., Ltd., Hagye Techno Town, Seoul, 139-230, Korea

    Bong-Yoon Kim

  3. alkaGENE Co., Ltd., Bundang Technopark, Seongnam-si, Kyeonggi-do, 463-760, Korea

    Hyung-Yook Jeon & Seung-Hyun Sung

  4. College of Pharmacy, Chungbuk National University, Cheongju, 361-763, Korea

    Chan-Su Park & Chong-Kil Lee

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  1. Seulmee Shin
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  2. Bong-Yoon Kim
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  4. Aeri Lee
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Correspondence to Kyungjae Kim.

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Shin, S., Kim, BY., Jeon, HY. et al. Expression system for production of bioactive compounds, recombinant human adiponectin, in the silk glands of transgenic silkworms. Arch. Pharm. Res. 37, 645–651 (2014). https://doi.org/10.1007/s12272-013-0298-9

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  • DOI: https://doi.org/10.1007/s12272-013-0298-9

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