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Analyse chromosomal integrierter HBV-DNA durch inverse nested PCR

  • Wissenschaft · Special: PCR & qPCR
  • DNA-Integration
  • Published:
BIOspektrum Aims and scope

Abstract

Chronic infection with human hepatitis B virus (HBV) causes about 887.000 deaths annually and is a major risk factor for liver cancer through as-yet unclear mechanisms. HBV DNA integrates into the host cell genome early during infection. HBV integration is not required for viral replication but contributes to subviral particle formation. It is also associated with HBV-induced liver cancer, so its detection, quantification, and characterization are key to understanding HBV-associated disease.

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Authors and Affiliations

  1. Zentrum für Infektiologie, Molekulare Virologie, Universitätsklinikum Heidelberg Center for Integrative Infectious Disease Research, Im Neuenheimer Feld 344, D-69120, Heidelberg, Deutschland

    Thomas Tu, Shirin Nkongolo & Stephan Urban

Authors
  1. Thomas Tu
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  2. Shirin Nkongolo
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  3. Stephan Urban
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Corresponding author

Correspondence to Stephan Urban.

Additional information

Thomas Tu 2004–2008 Studium der Biomedizinischen Wissenschaften an der Universität Adelaide, Australien; dort 2008–2013 Promotion in Biomedizin bei Prof. Dr. A. Jilbert. 2012–2015 Postdoktorand an der Universität Sydney, Australien, bei Prof. Dr. N. Shackel. Seit 2015 Postdoktorand an der Universität Heidelberg bei Prof. Dr. S. Urban.

Shirin Nkongolo 2008–2015 Medizinstudium an den Universitäten Heidelberg und Montpellier, Frankreich. 2012–2015 Promotion in Medizin an der Universität Heidelberg bei Prof. Dr. S. Urban. Seit 2016 Ärztin an der Universitätsklinik Heidelberg und Postdoktorandin an der Universität Heidelberg bei Prof. Dr. S. Urban.

Stephan Urban 1982–1992 Chemie- und Biochemiestudium an der Universität Tübingen. 1992–1995 Promotion am Max-Planck-Institut für Biochemie in Martinsried bei Prof. Dr. Dr. P. H. Hofschneider. 1995–2000 Postdoktorand am ZMBH der Universität Heidelberg bei Prof. Dr. H. Schaller. 2000 Habilitation in Molekularbiologie an der Universität Heidelberg; dort 2001–2008 Arbeitsgruppenleiter Molekulare Virologie, 2008–2014 apl. Professor an der Biologischen Fakultät und seit 2014 W3-Professor für Translationale Virologie.

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Tu, T., Nkongolo, S. & Urban, S. Analyse chromosomal integrierter HBV-DNA durch inverse nested PCR. Biospektrum 25, 282–284 (2019). https://doi.org/10.1007/s12268-019-1047-5

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  • DOI: https://doi.org/10.1007/s12268-019-1047-5

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