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N-acetylcysteine Plus Deferoxamine Improves Cardiac Function in Wistar Rats After Non-reperfused Acute Myocardial Infarction

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Abstract

The antioxidant N-acetycysteine can turn into a prooxidant molecule in presence of iron ions. Thus, our goal was to test if the association of N-acetylcysteine (NAC) and an iron chelator (deferoxamine—DFX) in a rodent model of acute myocardial infarction (AMI) improves cardiac function. Male Wistar rats were subjected to a SHAM surgery or AMI. The animals were randomized: vehicle, NAC (25 mg/kg for 28 days), DFX (40 mg/kg for 7 days), or NAC plus DFX (NAC plus DFX, respectively). Animals were killed 28 days after the AMI. Animals treated with NAC/DFX showed an increase in left ventricular ejection fraction at 28 days when compared with vehicle group (45.2 ± 10.9 % vs. 34.7 ± 8.7 %, p = 0.03). Antioxidant effect of NAC/DFX treatment decreased 4-hydroxynonenal when compared to AMI group (p = 0.06). In conclusion, we showed beneficial effect of NAC/DFX association in improving left ventricle function in an experimental AMI.

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Abbreviations

AMI:

Acute myocardial infarction

DFX:

Deferoxamine

EF:

Ejection fraction

FS:

Fractional shortening

HNE:

Hydroxynonenal

LV:

Left ventricular

LVDD:

Left ventricular diastolic diameter

LVSD:

Left ventricular systolic diameter

NAC:

N-acetylcysteine

PE:

Perimeter of the endocardium

ROS:

Reactive oxygen species

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Funding

This work was supported in part by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Secretaria de Ciência e Tecnologia da Fundação de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS # 11/1970-3), and Fundo de Incentivo à Pesquisa e Eventos do Hospital de Clínicas de Porto Alegre (FIPE-HCPA).

Animal Study

No human studies were carried out by the authors for this article.

All procedures followed during this investigation are in accordance with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85-23, revised 1996), and the experimental protocol was approved by the Ethics Committee of the Hospital de Clinicas de Porto Alegre (CEUA) by the number 110202.

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Authors and Affiliations

  1. Cardiovascular Research Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos 2350, Porto Alegre, RS, 90035-003, Brazil

    Amanda Phaelante, Luís Eduardo Rohde, Amanda Lopes, Virgílio Olsen, Santiago Alonso Leitão Tobar, Carolina Cohen, Nidiane Martinelli, Andréia Biolo, Nadine Clausell & Michael Andrades

  2. Post-Graduate Program in Cardiology and Cardiovascular Science, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Amanda Phaelante, Luís Eduardo Rohde, Andréia Biolo, Nadine Clausell & Michael Andrades

  3. Departamento de Medicina, Universidade do Extremo Sul Catarinense, Avenida Universitária, 1105, Criciúma, SC, 88806-000, Brazil

    Felipe Dal-Pizzol

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  1. Amanda Phaelante
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Correspondence to Michael Andrades.

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Associate Editor Paul J. R. Barton oversaw the review of this article

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Phaelante, A., Rohde, L.E., Lopes, A. et al. N-acetylcysteine Plus Deferoxamine Improves Cardiac Function in Wistar Rats After Non-reperfused Acute Myocardial Infarction. J. of Cardiovasc. Trans. Res. 8, 328–337 (2015). https://doi.org/10.1007/s12265-015-9633-5

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