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Determinants of Delayed Preconditioning Against Myocardial Stunning in Chronically Instrumented Pigs

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Abstract

To test the hypothesis that a critical stenosis prevents delayed preconditioning against stunning, studies were conducted in pigs chronically instrumented with occluders and segment-shortening crystals. In the setting of a critical stenosis, a preconditioning stimulus of repetitive brief occlusions resulted in infarction. Thereafter, a single 10-min occlusion was used as the preconditioning stimulus. Delayed preconditioning against stunning was documented on subsequent days by the deficit-of-function following brief repetitive occlusions. In contrast to experiments in the naïve heart, the deficit-of-function improved on the day after a single 10-min occlusion (from 60 ± 14 to 24 ± 6 arbitrary units, p = 0.003) and similar improvement occurred when reperfusion was performed through a critical stenosis (32 ± 6 units, p = 0.02 vs. naïve and p = 0.34 vs. no stenosis). Delayed preconditioning also reduced the frequency of ventricular fibrillation and produced a fourfold increase in both calcium-dependent and calcium-independent NOS activity. Thus, a critical stenosis did not prevent delayed preconditioning against stunning.

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Acknowledgements

I would like to thank Edward O. McFalls, M.D., Ph.D. and Joseph Sikora for performing the myocardial NOS activity assays, Elaine Granica for her expert technical assistance with conduct and analysis of these studies, and Anne Coe for her assistance with the completion of this manuscript. I would also like to thank John M. Canty, Jr., M.D. for his helpful comments on the draft of this manuscript. This study is supported by the Department of Veterans Affairs; the American Heart Association; and the National Heart, Lung and Blood Institute.

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  1. VA Western New York Health Care System at Buffalo, the Center for Research in Cardiovascular Medicine and the Division of Cardiovascular Medicine, Department of Medicine, University at Buffalo, Buffalo, NY, USA

    James A. Fallavollita

  2. Department of Medicine/Cardiology, University at Buffalo, Biomedical Research Building, Room 349, 3435 Main Street, Buffalo, NY, 14214, USA

    James A. Fallavollita

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Correspondence to James A. Fallavollita.

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Fallavollita, J.A. Determinants of Delayed Preconditioning Against Myocardial Stunning in Chronically Instrumented Pigs. J. of Cardiovasc. Trans. Res. 2, 71–80 (2009). https://doi.org/10.1007/s12265-008-9081-6

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  • DOI: https://doi.org/10.1007/s12265-008-9081-6

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