Abstract
Objective
A calcium-activated chloride current (I Cl(Ca)) has been observed in medium-sized sensory neurons of the dorsal root ganglion (DRG). Axotomy of the sciatic nerve induces a similar current in the majority of medium and large diameter neurons. Our aim is to identify the molecule(s) underlying this current.
Methods
Using conventional and quantitative RT-PCR, we examined the expression in DRG of members of three families of genes, which have been shown to have I Cl(Ca) current inducing properties.
Results
We showed the detection of transcripts representing several members of these families, i.e. chloride channel calcium-activated (CLCA), Bestrophin and Tweety gene families in adult DRG, in the normal state and 3 d after sciatic nerve section, a model for peripheral nerve injury.
Conclusion
Our analysis revealed that that mBest1 and Tweety2 appear as the best candidates to play a role in the injury-induced I Cl(Ca) in DRG neurons.
摘要
目的
在背根神经节 (dorsal root ganglion, DRG) 中等大小感觉神经元中可以观察到钙激活氯离子流 (I Cl(Ca))。 在坐骨神经损伤模型中, 在大多数大中神经元上诱导出类似的氯离子流。 本文旨在探讨引起这个离子流的分子基础。
方法
使用常规的定量 RT-PCR 方法检测在 DRG 中三个基因家族的表达, 这三个基因家族都具有诱导 I Cl(Ca) 的特点。
结果
在成年小鼠的DRG中, 分别显示了在正常状态和坐骨神经损伤3天后 CLCA, Bestrophin 和Tweety基因家族成员的转录产物。
结论
MBestl和Tweety2可能在损伤诱导的DRG神经元I Cl(Ca)中发挥作用。
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Al-Jumaily, M., Kozlenkov, A., Mechaly, I. et al. Expression of three distinct families of calcium-activated chloride channel genes in the mouse dorsal root ganglion. Neurosci. Bull. 23, 293–299 (2007). https://doi.org/10.1007/s12264-007-0044-8
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DOI: https://doi.org/10.1007/s12264-007-0044-8