Abstract
Gold nanoparticles differently affect cells depending on physical parameters (shape, size, aspect ratio, etc.). Therefore, it is essential to analyze shape- and concentration-dependent effects on cells and use them safely because new materials can simultaneously have both potential and threats. This research investigated the gold nanorod’s (GNR) shape effect and the concentration criteria on cell viability and why GNR promotes cell proliferation. Unlike 10-nm and 60-nm gold nanospheres, GNR of 3.4 aspect ratio generated intracellular reactive oxygen species (ROS), and ROS affected cell viability depending on concentrations. GNRs between 0.75 pM and 37 pM produced trace ROS, which promoted HDFn (human dermal fibroblasts, neonatal) cell viability. GNRs of 7.5 nM or more produced more ROS, which reduced HDFn cell viability. On the other hand, GNRs around 0.745 nm promoted HeLa cell viability. GNRs of 3.75 nM or more repressed HeLa cell viability. Hydrogen peroxide of 0.01 and 0.1 µM promoted HDFn cell viability by 7% and 9.9%. This observation could speculate that GNR-generated ROS promoted cell proliferation via activated the ERK1/2 signaling pathway. Therefore, picomolar GNRs could be used to enhance skin cell viability in cosmetics and wound healing. On the other hand, nanomolar GNRs could be applied to kill cancer cells.
Similar content being viewed by others
References
Park, C. R., W. J. Rhee, K. W. Kim, and B. H. Hwang (2019) Colorimetric biosensor using dual-amplification of enzyme-free reaction through universal hybridization chain reaction system. Biotechnol. Bioeng. 116: 1567–1574.
Lee, W. and B. H. Hwang (2020) Plasmonic biosensor controlled by DNAzyme for on-site genetic detection of pathogens. Biotechnol. J. 15: e1900329.
Lim, S. H., Y. C. Ryu, and B. H. Hwang (2021) Aptamer-immobilized gold nanoparticles enable facile and on-site detection of Staphylococcus aureus. Biotechnol. Bioprocess Eng. 26: 107–113.
Kim, S. H., Y. C. Ryu, H. M. D. Wang, and B. H. Hwang (2020) Optimally fabricated chitosan particles containing ovalbumin induced cellular and humoral immunity in immunized mice. Biotechnol. Bioprocess Eng. 25: 681–689.
Paithankar, D., B. H. Hwang, G. Munavalli, A. Kauvar, J. Lloyd, R. Blomgren, L. Faupel, T. Meyer, and S. Mitragotri (2015) Ultrasonic delivery of silica-gold nanoshells for photothermolysis of sebaceous glands in humans: nanotechnology from the bench to clinic. J. Control. Release. 206: 30–36.
Ryu, Y. C., K. A. Kim, B. C. Kim, H. M. D. Wang, and B. H. Hwang (2021) Novel fusion peptide-mediated siRNA delivery using self-assembled nanocomplex. J. Nanobiotechnology. 19: 44.
Laurent, S., D. Forge, M. Port, A. Roch, C. Robic, L. Vander Elst, and R. N. Muller (2008) Magnetic iron oxide nanoparticles: synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications. Chem. Rev. 108: 2064–2110. (Erratum published 2010, Chem. Rev. 110: 2574).
Khan, I., K. Saeed, and I. Khan (2019) Nanoparticles: properties, applications and toxicities. Arab. J. Chem. 12: 908–931.
Xie, X., J. Liao, X. Shao, Q. Li, and Y. Lin (2017) The effect of shape on cellular uptake of gold nanoparticles in the forms of stars, rods, and triangles. Sci. Rep. 7: 3827.
Woźniak, A., A. Malankowska, G Nowaczyk, B. F. Grześkowiak, K. Tuśnio, R. Słomski, A. Zaleska-Medynska, and S. Jurga (2017) Size and shape-dependent cytotoxicity profile of gold nanoparticles for biomedical applications. J. Mater. Sci. Mater. Med. 28: 92.
Chithrani, B. D., A. A. Ghazani, and W. C. Chan (2006) Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano Lett. 6: 662–668.
Qiu, Y., Y. Liu, L. Wang, L. Xu, R. Bai, Y. Ji, X. Wu, Y. Zhao, Y. Li, and C. Chen (2010) Surface chemistry and aspect ratio mediated cellular uptake of Au nanorods. Biomaterials. 31: 7606–7619.
Singh, P., S. Pandit, V. R. S. S. Mokkapati, A. Garg, V. Ravikumar, and I. Mijakovic (2018) Gold nanoparticles in diagnostics and therapeutics for human cancer. Int. J. Mol. Sci. 19: 1979.
Bagheri, S., M. Yasemi, E. Safaie-Qamsari, J. Rashidiani, M. Abkar, M. Hassani, S. A. Mirhosseini, and H. Kooshki (2018) Using gold nanoparticles in diagnosis and treatment of melanoma cancer. Artif. Cells Nanomed. Biotechnol. 46(sup1): 462–471.
Chuang, S. M. Y. H. Lee, R. Y. Liang, G. D. Roam, Z. M. Zeng, H. F. Tu, S. K. Wang, and P. J. Chueh (2013) Extensive evaluations of the cytotoxic effects of gold nanoparticles. Biochim. Biophys. Acta. 1830: 4960–4973.
Guerrero-Florez, V., S. C. Mendez-Sanchez, O. A. Patrón-Soberano, V. Rodríguez-González, D. Blach, and F. MartínezO (2020) Gold nanoparticle-mediated generation of reactive oxygen species during plasmonic photothermal therapy: a comparative study for different particle sizes, shapes, and surface conjugations. J. Mater. Chem. B. 8: 2862–2875.
Mahmoud, N. N., L. M. Al-Kharabsheh, E. A. Khalil, and R. Abu-Dahab (2019) Interaction of gold nanorods with human dermal fibroblasts: cytotoxicity, cellular uptake, and wound healing. Nanomaterials (Basel) 9: 1131. (Erratum published 2021, Nanomaterials (Basel). 11: 1364)
Alkilany, A. M., P. K. Nagaria, C. R. Hexel, T. J. Shaw, C. J. Murphy, and M. D. Wyatt (2009) Cellular uptake and cytotoxicity of gold nanorods: molecular origin of cytotoxicity and surface effects. Small. 5: 701–708.
Day, R. M. and Y. J. Suzuki (2006) Cell proliferation, reactive oxygen and cellular glutathione. Dose Response. 3: 425–442.
Cao, Y. (2018) Applications of cellulose nanomaterials in pharmaceutical science and pharmacology. Express Polym. Lett. 12: 768–780.
Milkovic, L., A. Cipak Gasparovic, M. Cindric, P. A. Mouthuy, and N. Zarkovic (2019) Short overview of ROS as cell function regulators and their implications in therapy concepts. Cells. 8: 793.
Shi, K., Z. Gao, T. Q. Shi, P. Song, L. J. Ren, H. Huang, and X. J. Ji (2017) Reactive oxygen species-mediated cellular stress response and lipid accumulation in oleaginous microorganisms: the state of the art and future perspectives. Front. Microbiol. 8: 793.
Schieber, M. and N. S. Chandel (2014) ROS function in redox signaling and oxidative stress. Curr. Biol. 24: R453–R462.
Pinto, M. C. X., A. H. Kihara, V. A. Goulart, F. M. Tonelli, K. N. Gomes, H. Ulrich, and R. R. Resende (2015) Calcium signaling and cell proliferation. Cell. Signal. 27: 2139–2149.
Hong, Z., J. A. Cabrera, S. Mahapatra, S. Kutty, E. K. Weir, and S. L. Archer (2014) Activation of the EGFR/p38/JNK pathway by mitochondrial-derived hydrogen peroxide contributes to oxygen-induced contraction of ductus arteriosus. J. Mol. Med. (Berl). 92: 995–1007.
Perillo, B., M. Di Donato, A. Pezone, E. Di Zazzo, P. Giovannelli, G. Galasso, G. Castoria, and A. Migliaccio (2020) ROS in cancer therapy: the bright side of the moon. Exp. Mol. Med. 52: 192–203.
Lingabathula, H. and N. Yellu (2016) Cytotoxicity, oxidative stress, and inflammation in human Hep G2 liver epithelial cells following exposure to gold nanorods. Toxicol. Mech. Methods. 26: 340–347.
Acknowledgements
This research was supported by Incheon National University Research Grant in 2018.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare no financial or commercial conflict of interest.
Neither ethical approval nor informed consent was required for this study.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic Supplementary Material
Rights and permissions
About this article
Cite this article
Lee, J., Hwang, B.H. Evaluation of the Effects, Causes, and Risks of Gold Nanorods Promoting Cell Proliferation. Biotechnol Bioproc E 27, 213–220 (2022). https://doi.org/10.1007/s12257-021-0161-7
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12257-021-0161-7