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Evaluation of the Effect of C-phycocyanin on Cultured Rat Primary Hippocampal Astrocytes Undergoing Trophic Factor Deprivation

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Abstract

Hippocampal astrocytes play a pivotal role in hippocampal functions. However, trophic factor deprivation during brain injury or neurodegenerative diseases leads to abnormal functions. C-phycocyanin (C-Pc) is an antioxidant compound derived from Spirulina. C-Pc has been studied as a neuroprotectant against brain damage and diseases, however, its effects on hippocampal astrocytes cultured under trophic factor deprivation are unclear. Presently, serum deprivation lowered the metabolic activities, decreasing NADPH diaphorase activity, and decreased production of superoxide dismutase in astrocytes. These effects were reversed after C-Pc treatment. The upregulated NADPH diaphorase activity in activated astrocytes resulted in the increased production of nitric oxide, which protected the hippocampal astrocytes from the harsh trophic factor-deprived environment.

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Acknowledgment

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (NRF-2016 R1D1A1B03931923) and INHA UNIVERSITY research grant.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Biological Engineering, Inha University, Incheon, 22212, Korea

    Su Jin Yoon, Jung Il Choi & Hwa Sung Shin

  2. Division of Polar Life Science, Korea Polar Research Institute, Incheon, 21990, Korea

    Seul Ki Min

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  1. Su Jin Yoon
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  2. Jung Il Choi
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Correspondence to Seul Ki Min or Hwa Sung Shin.

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Yoon, S.J., Choi, J.I., Min, S.K. et al. Evaluation of the Effect of C-phycocyanin on Cultured Rat Primary Hippocampal Astrocytes Undergoing Trophic Factor Deprivation. Biotechnol Bioproc E 24, 870–875 (2019). https://doi.org/10.1007/s12257-019-0102-x

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