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Isolation and characterization of collagen from marine fish (Thunnus obesus)

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Abstract

In the present study, we isolated collagen from Thunnus obesus bone, which was physiochemically characterized. Two different kinds of methods were used to isolate the collagen; they are the Acid Soluble Collagen (ASC) and Acid Soluble Enzyme Collagen (ASEC) methods. The isolated collagen was characterized with Fourier Transform Infrared Spectroscopy (FT-IR), SDS-polyacrylamide gel electrophoresis (SDS-PAGE), Optical Microscopy (OM) and Scanning Electron Microscopy (SEM). FT-IR results revealed the presence of collagen. SEM and OM results depicted that collagen was in the form of fiber sponge-like scaffolds. The isolated collagen scaffold was checked with pre-osteoblast (MC3T3-E1) cell line for biocompatibility. The in vitro results revealed that the collagen scaffolds were highly biocompatible and nontoxic in nature. Herewith, we are suggesting that marine fish-derived collagen will be an excellent material for leather, film industry, pharmaceutical, cosmetics, biomedical and food applications.

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Authors and Affiliations

  1. Department of Chemistry, Pukyong National University, Busan, 608-737, Korea

    Hee-Seok Jeong & Se-Kwon Kim

  2. Marine Bioprocess Research Center, Pukyong National University, Busan, 608-737, Korea

    Jayachandran Venkatesan & Se-Kwon Kim

Authors
  1. Hee-Seok Jeong
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  2. Jayachandran Venkatesan
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  3. Se-Kwon Kim
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Correspondence to Se-Kwon Kim.

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Both authors contributed equally to this work.

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Jeong, HS., Venkatesan, J. & Kim, SK. Isolation and characterization of collagen from marine fish (Thunnus obesus). Biotechnol Bioproc E 18, 1185–1191 (2013). https://doi.org/10.1007/s12257-013-0316-2

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  • DOI: https://doi.org/10.1007/s12257-013-0316-2

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