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Apoptotic Effects of Quercitrin on DLD-1 Colon Cancer Cell Line

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Pathology & Oncology Research

Abstract

Quercetin, which is the most abundant bioflavonoid compound, is mainly present in the glycoside form of quercitrin. Although different studies indicated that quercitrin is a potent antioxidant, the action of this compound is not well understood. In this study, we investigated whether quercitrin has apoptotic and antiproliferative effects in DLD-1 colon cancer cell lines. Time and dose dependent antiproliferative and apoptotic effects of quercitrin were subsequently determined by WST-1 cell proliferation assay, lactate dehydrogenase (LDH) cytotoxicity assay, detection of nucleosome enrichment factor, changes in caspase-3 activity, loss of mitochondrial membrane potential (MMP) and also the localization of phosphatidylserine (PS) in the plasma membrane. There were significant increases in caspase-3 activity, loss of MMP, and increases in the apoptotic cell population in response to quercitrin in DLD-1 colon cancer cells in a time- and dose-dependent manner. These results revealed that quercitrin has antiproliferative and apoptotic effects on colon cancer cells. Quercitrin activity supported with in vivo analyses could be a biomarker candicate for early colorectal carcinoma.

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Acknowledgments

This work was supported by the Istanbul University Scientific Research Projects; 31162. We would like to thank to Mr. David Chapman for English editing.

Conflict of interest

The authors declare that they have no financial disclosures or conflicts of interest.

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Authors and Affiliations

  1. Istanbul University, Institute of Experimental Medical Research, Department of Molecular Medicine, Capa, Istanbul, Turkey

    Zeynep Birsu Cincin, Elif Sinem Bireller & Bedia Cakmakoglu

  2. Izmir Institute of Technology, Science Faculty, Department of Molecular Biology and Genetics, Urla, 35430, Izmir, Turkey

    Miray Unlu & Yusuf Baran

  3. Kastamonu University, Department of Biology, Kastamonu, Turkey

    Bayram Kiran

Authors
  1. Zeynep Birsu Cincin
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  2. Miray Unlu
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  3. Bayram Kiran
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  4. Elif Sinem Bireller
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  5. Yusuf Baran
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  6. Bedia Cakmakoglu
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Correspondence to Bedia Cakmakoglu.

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Cincin, Z.B., Unlu, M., Kiran, B. et al. Apoptotic Effects of Quercitrin on DLD-1 Colon Cancer Cell Line. Pathol. Oncol. Res. 21, 333–338 (2015). https://doi.org/10.1007/s12253-014-9825-3

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  • DOI: https://doi.org/10.1007/s12253-014-9825-3

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