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Down-Regulated Expression of HSP70 in Correlation with Clinicopathology of Cholangiocarcinoma

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Pathology & Oncology Research

Abstract

Cholangiocarcinoma is a crucial health problem in northeast Thailand. Although rare, it is a highly fatal disease and the prognosis of CCA patients is very poor. To determine if expression of specific genes is useful for diagnosis and prognosis for CCA. We examined the expression of HSP70, HSP90, RB1, cyclin D1, and HDAC6 in 50 resections of human CCA tissues by quantitative real-time PCR. The expression of HSP70, RB1, and HDAC6 was “dominant down-regulation,” while the expression of cyclin D1 and HSP90 was “dominant up-regulation.” There were no correlations between RB1, cyclin D1, HSP90, and clinicopathological parameters such as status, histology type, histological grading, stage of CCA, and metastasis. A significant association was found between HDAC6 and CCA staging (p = 0.000), CCA gross type and HSP70 (p = 0.046) as well as RB1 expression (p = 0.046). Patients with down-regulation of HSP70 had significantly poorer prognosis than those in the up-regulation group (p = 0.002). Expression of HSP70 may be useful as a new prognostic marker for CCA.

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Abbreviations

CCA:

cholangiocarcinoma

RB1:

retinoblastoma 1

HSP70:

heat shock protein 70

HSP90:

heat shock protein 90

HDAC6:

histone deacetylase 6

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Acknowledgments

This work was supported by the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission and the Thailand Research Fund through the Royal Golden Jubilee PhD program (Grant No. PHD/0139/2551) to Miss Sirintip Boonjaraspinyo and Associate Professor Thidarut Boonmars. The authors also gratefully acknowledge partial financial support from the Grant-in-Aid for Scientific Research (21590463) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We also wish to thank the Department of Parasitology, Liver Fluke and Cholangiocarcinoma Research Center, Animal Experimental Unit, Faculty of Medicine, Khon Kaen University, for their assistance. We also thank Stewart Sell, MD, Ordway Research Institute for his review of the manuscript.

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Correspondence to Thidarut Boonmars.

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Boonjaraspinyo, S., Boonmars, T., Kaewkes, S. et al. Down-Regulated Expression of HSP70 in Correlation with Clinicopathology of Cholangiocarcinoma. Pathol. Oncol. Res. 18, 227–237 (2012). https://doi.org/10.1007/s12253-011-9432-5

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