Skip to main content

Advertisement

SpringerLink
Log in

Antiviral activity of the effective monomers from folium isatidis against influenza virus in vivo

  • Published:
Virologica Sinica

Abstract

In order to evaluate the anti-influenza virus activity of the effective monomer from Folium Isatidis (FI) in vivo, we established mice model with viral pneumonia and divided them into 3 different dose groups, then observed their lung indexes, pulmonary pathological changes, pulmonary virus hemagglitination titers, living time and death rates. The results showed that the monomer could reduce the pulmonary index from 2.64 to 1.93, 1.63 and 1.40 (P<0.01) and decrease the hemagglitination titer from 1.15 to 0.84, 0.70 and 0.59 (P<0.01). In addition, different groups of FI could significantly lessen the mortality rate from 100% to 30%, 25% and 15%, and prolong the living time from 5.1d to 6.5d, 8.4d and 8.9d respectively(P<0.01). The high dose (75 mg/kg/d) has the similar effect with 100 mg/kg/d dose of virazole(P>0.05), and more effective than 200 mg/kg/d dose of antiviral liquor (P<0.05).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Barr I G, Hurt A C, Deed N,et al. 2007a. The emergence of adamantane resistance in influenza A (H1) viruses in Australia and regionally in 2006. Antiviral Res, 75: 173–176.

    Article  CAS  PubMed  Google Scholar 

  2. Barr I G, Hurt A C, Iannello P,et al. 2007b. Increased adamantane resistance in influenza A (H3) viruses in Australia and neighbouring countries in 2005. Antiviral Res, 73: 112–117.

    Article  CAS  PubMed  Google Scholar 

  3. Bright R A, Medina M J, Xu X,et al. 2005. Incidence of adamantane resistance among influenza A (H3N2) viruses isolated worldwide from 1994 to 2005: a cause for concern. Lancet, 366: 1175–1181.

    Article  CAS  PubMed  Google Scholar 

  4. Bright R A, Shay D K, Shu B,et al. 2006. Adamantane resistance among influenzaAviruses isolated early during the 2005–2006 influenza season in the United States. JAMA, 295: 891–894.

    Article  CAS  PubMed  Google Scholar 

  5. Cheung C L, Rayner J M, Smith G J,et al. 2006. Distribution of amantadine-resistant H5N1 avian influenza variants in Asia. J Infect Dis, 193: 1626–1629.

    Article  CAS  PubMed  Google Scholar 

  6. Deyde V M, Okomo-Adhiambo M, Sheu T G,et al. 2009. Pyrosequencing as a tool to detect molecular markers of resistance to neuraminidase inhibitors in seasonal influenza A viruses. Antivir Res, 81: 16–24.

    Article  CAS  PubMed  Google Scholar 

  7. Garman E, Laver G. 2004. Controlling influenza by inhibiting the virus’s neu-raminidase. Curr Drug Targets, 5: 119–136.

    Article  CAS  PubMed  Google Scholar 

  8. Hayden F G, Hay A J. 1992. Emergence and transmission of influenza A viruses resistant to amantadine and rimantadine. Curr Top Microbiol Immunol, 176: 119–130.

    CAS  PubMed  Google Scholar 

  9. Hayden F, Klimov A, Tashiro M,et al. 2005. Neuraminidase inhibitor susceptibility network position statement: antiviral resistance in influenza A/H5N1 viruses. Antiviral Ther, 10: 873–877.

    CAS  Google Scholar 

  10. Ilyushina N A, Govorkova E A, Webster R G. 2005. Detection of amantadine-resistant variants among avian influenza viruses isolated in North America and Asia. Virology, 341: 102–106.

    Article  CAS  PubMed  Google Scholar 

  11. Puthavathana P, Auewarakul P, Charoenying P C,et al. 2005. Molecular characterization of the complete genome of human influenza H5N1virus isolates from Thailand. J Gen Virol, 86: 423–433

    Article  CAS  PubMed  Google Scholar 

  12. Palese P. 2004. Influenza: old and new threats. Nat Med, 10: S82–S87.

    Article  CAS  PubMed  Google Scholar 

  13. Saito R, Li D, Suzuki Y,et al. 2007. High prevalence of amantadine-resistance influenza A (H3N2) in six prefectures, Japan, in the 2005–2006 season. J Med Virol, 79: 1569–1576.

    Article  CAS  PubMed  Google Scholar 

  14. Sheu T G, Deyde V M, Okomo-Adhiambo M,et al. 2008. Surveillance for neuraminidase inhibitor resistance among human influenza A and B viruses circulating worldwide from 2004–2008. Antimicrob Agents Chemother, 52: 3284–3292.

    Article  CAS  PubMed  Google Scholar 

  15. Shiraishi K, Mitamura K, Sakai-Tagawa Y,et al. 2003. High frequency of resistant viruses harboring different muta-tions in amantadine-treated children with influenza. J Infect Dis, 188: 57–61.

    Article  PubMed  Google Scholar 

  16. Wutzler P, Kossow K D, Lode H,et al. 2004. Antiviral treatment and prophylaxis of influenza in primary care: German recommenda-tions. J Clin Virol, 31: 84–91.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Pharmacy, South-Central University for Nationalities, Wuhan, 430074, China

    Zhao Liu

  2. Medical Virology Institute, Wuhan University, Wuhan, 430071, China

    Zhan-qiu Yang & Hong Xiao

Authors
  1. Zhao Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhan-qiu Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hong Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhao Liu.

Additional information

Foundation item: Natural Science Foundation of South Central University for Nationalities (YZQ05011)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, Z., Yang, Zq. & Xiao, H. Antiviral activity of the effective monomers from folium isatidis against influenza virus in vivo. Virol. Sin. 25, 445–451 (2010). https://doi.org/10.1007/s12250-010-3142-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12250-010-3142-0

Key words

Search

Navigation