Abstract
The activity of antagonistic substances produced by Pseudomonas aeruginosa and Lactobacillus acidophilus against the planktonic and sessile populations of Staphylococcus aureus strains was demonstrated. The strongest effects were caused by probiotic L. acidophilus strain — bacteriocin-like inhibitory substances (BLIS) positive. However, the S. aureus A3 growth, adhesion and biofilm formation was also limited by cell-free supernatant of L. acidophilus H-1 (BLIS negative). Moreover, competitive direct interactions were observed between staphylococci and the above bacteria, which influenced the formation of dualspecies aggregates on the surface.
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Abbreviations
- ATCC:
-
American Type Culture Collection
- BLIS:
-
bacteriocin-like inhibitory substances
- MHB:
-
Mueller-Hinton broth
- MRS:
-
De Man-Rogosa-Sharp (broth)
- MRSA:
-
methicillin-resistant S. aureus
- NCTC:
-
National Culture Type Collection
- MTT:
-
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
- TSB:
-
tryptic soya broth
- E.c.:
-
Escherichia coli
- P.a.:
-
Pseudomonas aeruginosa
- E.f.:
-
Enterococcus faecalis
- P.m.:
-
Proteus mirabilis
- L.a.:
-
Lactobacillus acidophilus
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Sadowska, B., Walencka, E., Wieckowska-Szakiel, M. et al. Bacteria competing with the adhesion and biofilm formation by Staphylococcus aureus . Folia Microbiol 55, 497–501 (2010). https://doi.org/10.1007/s12223-010-0082-x
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DOI: https://doi.org/10.1007/s12223-010-0082-x