Abstract
The antimicrobial susceptibility of 30 clinical and 30 food Bacillus cereus isolates was determined. All isolates were susceptible to streptomycin, ciprofloxacin and gentamicin, 90 % of them to clindamycin and vancomycin, and 67 % to erythromycin. All isolates were resistant to amoxicillin with clavulanic acid, ampicillin, cefotaxime, ciprofloxacin, cloxacillin, cefotaxime with clavulanic acid and penicillin. The MIC values (determined by E-tests) were 48–256 mg/L for ampicillin, 0.19–1.5 mg/L for gentamicin, 0.125–1.0 mg/L for clindamycin, 0.047–4.0 mg/L for erythromycin and 1.5–16 mg/L for vancomycin. The MICs 4.6–18.75 g/L were observed for penicillin using the microdilution method. The presence of metallo-β-lactamases was detected by E-test for 100 % of strains. Nonhemolytic diarrheal enterotoxin (NHE) was produced by 98.3 % of strains, while 31.7 % of them produced hemolytic diarrheal enterotoxin (HBL). Clinical isolates produced 10 % more HBL than food isolates. The psychrotrophic strains isolated from food samples produced NHE at 6.5 °C in 73 % of cases.
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Abbreviations
- BHI:
-
brain heart infusion (broth)
- CGY:
-
cereus glucose yeast (broth)
- HBL:
-
hemolytic diarrheal enterotoxin
- NHE:
-
nonhemolytic diarrheal enterotoxin
- INT:
-
p-iodonitrotetrazolium violet
- MBC:
-
minimum bactericidal concentration
- MIC:
-
minimum inhibitory concentration
- MIC50 :
-
MIC at which a 50 % inhibition was found
- MIC90 :
-
MIC at which a 90 % inhibition was found
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Godič Torkar, K., Seme, K. Antimicrobial susceptibility, β-lactamase and enterotoxin production in Bacillus cereus isolates from clinical and food samples. Folia Microbiol 54, 233–238 (2009). https://doi.org/10.1007/s12223-009-0037-2
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DOI: https://doi.org/10.1007/s12223-009-0037-2