Abstract
Microbial-Induced Calcite Precipitation (MICP) has recently emerged as a sustainable technique for soil improvement. This paper aims to study the effectiveness of MICP in improving the shear strength and reducing the hydraulic conductivity of soils. A species of Bacillus group, B. megaterium was used to trigger the calcite precipitation. The experimental variables included soil types (tropical residual soil and sand), soil densities (85%, 90%, and 95% of their respective maximum densities), and treatment conditions (untreated, treated with cementation reagents only, treated with B. megaterium only, and treated with B. megaterium and cementation reagents). The results showed that MICP could effectively improve shear strength and reduce hydraulic conductivity for both residual soil and sand. The improvements, however, varied with soil densities, soil types, and treatment conditions. With MICP treatment, the improvement ratios in shear strength of the residual soil specimens were significantly higher (1.41–2.64) than those of the sand specimens (1.14–1.25). On the contrary, the sand specimens resulted in greater hydraulic conductivity reduction ratios (0.09–0.15) than those of the residual soil specimens (0.26–0.45). These observations can be explained by the particle-particle contacts per unit volume and pore spaces in the soil specimens. Both soil specimens when treated with cementation reagents only exhibited slight alterations in the shear strength (ranging from 1.06–1.33) and hydraulic conductivity (ranging from 0.69–0.95). The results implied that natural calcite forming microorganisms only exist for insignificant amount. The amount of calcite precipitated in the treated residual soil specimens ranged from 1.080% to 1.889%. The increments of calcite content in the treated sand specimens were comparatively higher, ranging from 2.661% to 6.102%. The results from Scanning Electron Microscope (SEM) analysis confirmed the experimental findings.
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References
Achal, V., Mukherjee, A., Basu, P. C., and Reddy, M. S. (2009). “Strain improvement of Sporosarcina Pasteurii for enhanced urease and calcite production.” J. Ind. Microbiol. Biotechnol., Vol. 36, No. 7, pp. 981–988.
Achal, V., Pan, X., and Özyurt, N. (2011). “Improved strength and durability of fly ash-amended concrete by microbial calcite precipitation.” Ecol. Eng., Vol. 37, No. 4, pp. 554–559.
Anagnostopoulos, C. and Hadjispyrou, S. (2004). “Laboratory study of an epoxy resin grouted sand.” Ground Improv., Vol. 8, No. 1, pp. 39–45.
ASTM (2000). ASTM D 4253-00, Standard test methods for maximum index density and unit weight of soils using a vibratory table, American Society for Testing and Materials.
ASTM (2000). ASTM D 4254-00, Standard test methods for minimum index density and unit weight of soils and calculation of relative density, American Society for Testing and Materials.
Basha, E. A., Hashim, R., Mahmud, H. B., and Muntohar, A. S. (2005). “Stabilization of residual soil with rice husk ash and cement.” Constr. Build. Mater., Vol. 19, No. 6, pp. 448–453.
Baskar, S., Baskar, R., Mauclaire, L., and Mckenzie, J. A. (2006). “Microbially induced calcite precipitation in culture experiments: Possible origin for stalactites in Sahastradhara caves, Dehradun, India.” Curr. Sci., Vol. 90, No. 1, pp. 58–64.
Baveye, P., Vandevivere, P., Hoyle, B., DeLeo, P., and de Lozada, D. S. (1998). “Environmental impact and mechanisms of the biological clogging of saturated soils and aquifer materials.” Crit. Rev. Environ. Sci. Technol., Vol. 28, No. 2, pp. 123–191.
Brassington, R. (1988). Field hydrogeology, Halsted Press, New York.
BSI (1990). British standard 1377: 1990, Methods of test for soils for civil engineering purposes, BSI Group, United Kingdom.
Castainer, S., Le Métayer-Levrel, G., and Perthuisot, J.-P. (1999). “Cacarbonates precipitation and limestone genesis — The microbiogeologist point of view.” Sediment. Geol., Vol. 126, Nos. 1–4, pp. 9–23.
Dejong, J. T., Fritzges, M. B., and Nüsslein, K. (2006). “Microbially induced cementation to control sand response to undrained shear.” J. Geotech. Geoenviron. Eng., Vol. 132, No. 11, pp. 1381–1392.
Dejong, J. T., Mortensen, B. M., Martinez, B. C., and Nelson, D. C. (2010). “Bio-mediated soil improvement.” Ecol. Eng., Vol. 36, No. 2, pp. 197–210.
De Muynck, W., De Belie, N., and Verstraete, W. (2010). “Microbial carbonate precipitation in construction materials: A review.” Ecol. Eng., Vol. 36, No. 2, pp. 118–136.
Ehrlich, H. L. (1999). “Past, present and future of biohydrometallurgy.” Process Metallurgy, R. Amils, and A. Ballester, eds., Elsevier, pp. 3–12.
Hammes, F., Boon, N., de Villiers, J., Verstraete, W., and Siciliano, S. D. (2003). “Strain-specific ureolytic microbial calcium carbonate precipitation.” Appl. Environ. Microbiol., Vol. 69, No. 8, pp. 4901–4909.
Harkes, M. P., Van Paassen, L. A., Booster, J. L., Whiffin, V. S., and Van Loosdrecht, M. C. M. (2010). “Fixation and distribution of bacterial activity in sand to induce carbonate precipitation for ground reinforcement.” Ecol. Eng., Vol. 36, No. 2, pp. 112–117.
Ho, M. H., and Chan, C. M. (2011). “Some mechanical properties of cement stabilized malaysian soft clay.” World Acad. Sci. Eng. Technol., Vol. 74, No., pp. 24–31.
Huat, B. B. K. (2006). “Deformation and shear strength characteristics of some tropical peat and organic soils.” Pertanika J. Sci. Technol., Vol. 14, Nos. 1–2, pp. 61–74.
Ivanov, V. and Chu, J. (2008). “Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ.” Rev. Environ. Sci. Biotechnol., Vol. 7, No. 2, pp. 139–153.
Karol, R. H. (2003). Chemical grouting and soil stabilization, M. Dekker, New York.
Kazemian, S. and Huat, B. B. K. (2009). “Assessment and comparison of grouting and injection methods in geotechnical engineering.” Eur. J. Sci. Res., Vol. 27, No. 2, pp. 234–247.
Kazemian, S., Huat, B. B. K., Prasad, A., and Barghchi, M. (2011). “A state of art review of peat geotechnical engineering perspective.” Int. J. Phys. Sci., Vol. 6, No. 8, pp. 1974–1981.
Krebs, R. D. and Walker, R. D. (1971). Highway materials, McGraw-Hill, New York.
Leonards, G. A. (1962). Foundation engineering, McGraw-Hill, New York.
Lian, B., Hu, Q., Chen, J., Ji, J., and Teng, H. (2006). “Carbonate biomineralization induced by soil bacterium Bacillus megaterium.” Geochim. Cosmochim. Acta, Vol. 70, No. 22, pp. 5522–5535.
Lu, W., Qian, C., and Wang, R. (2010). “Study on soil solidification based on microbiological precipitation of CaCO3.” Sci. China Technol. Sci., Vol. 53, No. 9, pp. 2372–2377.
Martinez, B. C., Barkouki, T. H., Dejong, J. D., and Ginn, T. R. (2011). “Upscaling of microbial induced calcite precipitation in 0.5 m columns experimental and modeling results.” Geo-Frontiers 2011, pp. 4049–4059.
Mitchell, J. K. and Santamarina, J. C. (2005). “Biological considerations in geotechnical engineering.” Journal Geotech. Geoenviron. Eng., Vol. 131, No. 10, pp. 1222–1233.
Okwadha, G. D. and Li, J. (2010). “Optimum conditions for microbial carbonate precipitation.” Chemosphere, Vol. 81, No. 9, pp. 1143–1148.
Peethamparan, S., Olek, J., and Diamond, S. (2009). “Mechanism of stabilization of Na-montmorillonite clay with cement kiln dust.” Cem. Concr. Res., Vol. 39, No. 7, pp. 580–589.
Qabany, A. A., Mortensen, B., Martinez, B., Soga, K., and Dejong, J. (2011). “Microbial carbonate precipitation correlation of s-wave velocity with calcite precipitation.” Geo-Frontiers 2011, pp. 3993–4001.
Stocks-Fischer, S., Galinat, J. K., and Bang, S. S. (1999). “Microbiological precipitation of CaCO3.” Soil Biol. Biochem., Vol. 31, No. 11, pp. 1563–1571.
Stoner, D. L., Watson, S. M., Stedtfeld, R. D., Meakin, P., Griffel, L. K., Tyler, T. L., Pegram, L. M., Barnes, J. M., and Deason, V. A. (2005). “Application of stereolithographic custom models for studying the impact of biofilms and mineral precipitation on fluid flow.” Appl. Environ. Microbiol., Vol. 71, No. 12, pp. 8721–8728.
Tan, L. P., Lee, C. Y., and Sivadass, T. (2008). “Parametric study of residual soil slope stability.” International Conference on Construction and Building Technology 2008, 16–20 Jun 2008, UniTen, Malaysia, pp. 33–42.
Van Paassen, L. A., Daza, C. M., Staal, M., Sorokin, D. Y., van der Zon, W., and van Loosdrecht, M. C. M. (2010). “Potential soil reinforcement by biological denitrification.” Ecol. Eng., Vol. 36, No. 2, pp. 168–175.
Van Tittelboom, K., De Belie, N., De Muynck, W., and Verstraete, W. (2010). “Use of bacteria to repair cracks in concrete.” Cem. Concr. Res., Vol. 40, No. 1, pp. 157–166.
Whiffin, V. S., van Paassen, L. A., and Harkes, M. P. (2007). “Microbial carbonate precipitation as a soil improvement technique.” Geomicrobiol. Journal, Vol. 24, No. 5, pp. 417–423.
Wittmann, C. and Krull, R. (2010). Biosystems engineering I: Creating superior biocatalysts, Advances in Biochemical Engineering / Biotechnology, Springer-Verlag Berlin Heidelberg, Berlin, Heidelberg.
Xanthakos, P. P., Abramson, L. W., and Bruce, D. A. (1994). Ground control and improvement, J. Wiley, New York.
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Soon, N.W., Lee, L.M., Khun, T.C. et al. Improvements in engineering properties of soils through microbial-induced calcite precipitation. KSCE J Civ Eng 17, 718–728 (2013). https://doi.org/10.1007/s12205-013-0149-8
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DOI: https://doi.org/10.1007/s12205-013-0149-8