Abstract
A novel aptamer solid-phase microextraction (SPME) fibre-based on poly (methacrylic acid-co-ethylene glycol dimethacrylate) for lysozyme-specific recognition has been successfully prepared. The prepared SPME fibres were characterised by various instrumental methods, including scanning electron microscopy, X-ray photoelectron spectroscopy and Fourier-transform infrared spectrometry. The results showed that the SPME fibre exhibited a porous structure, which is beneficial for the enrichment of the aptamer. The solid-phase microextraction-high-performance liquid chromatography-fluorescence detection (SPME-HPLC-FLD) with the superior selectivity and sensitivity responded linearly to the concentration of lysozyme over the 0.025–10 mg/L range, and the limit of detection was low at 6.8 μg/L, with a linearity of 0.9995. The method showed excellent reproducibility, and the relative standard deviations (RSDs) were less than 5.5%, which facilitated the analysis. The method was applied for the analysis of lysozyme in cow milk and papaya samples, and the recoveries ranged from 86.7–93.5% with RSDs of 4.3–6.1%, which clearly state that the proposed lysozyme aptamer SPME-HPLC-FLD method is a promising analytical tool for the analysis of lysozyme in food samples.
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Funding
This study was financed by the National Natural Science Foundation of China (21505115), the Top Scientific and Technological Talents in Universities of Guizhou Province (KY2018078), the Higher Education Improvement Project of Guizhou Province (2017014) and the Professor Programs Foundation of Xingyi Normal University for Nationalities.
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Qianchun Zhang declares that he has no conflict of interest. Junyu Chen declares that he has no conflict of interest. Yutong Wang declares that she has no conflict of interest. Guangping Xia declares that she has no conflict of interest. Yuguo Zheng declares that he has no conflict of interest. Xingyi Wang declares that she has no conflict of interest. Yun Wu declares that she has no conflict of interest. Changbo Zhang declares that he has no conflict of interest.
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Zhang, Q., Chen, J., Wang, Y. et al. Preparation and application of lysozyme aptamer fibre for specific recognition of lysozyme in food samples. Food Anal. Methods 14, 1464–1471 (2021). https://doi.org/10.1007/s12161-021-01993-7
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DOI: https://doi.org/10.1007/s12161-021-01993-7