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Size Exclusion Coupled to Reversed Phase Liquid Chromatography for the Characterization of Cranberry Products

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Abstract

A combination of solvent extraction, size exclusion purification, and reversed-phase liquid chromatography with UV absorption and fluorescence detection was here used for the determination of flavan-3-ols and related oligomeric compounds in cranberry products. The method relied on robust, simple, and inexpensive instrumental equipment present in most of routine analytical laboratories. Sample extracts in methanol/water/hydrochloric acid solution (60:39:1, v/v/v) were subjected to size-exclusion chromatography (SEC) using Sephadex LH-20 as the sorbent to separate simple phenolic and oligomeric components. As a novel aspect of this work, the implementation of the SEC process in a reusable packed column was practically and economically highly advantageous. Fractions were analyzed by reversed-phase chromatography using a C18 core-shell analytical column and 0.1% formic acid aqueous solution and methanol as the components of the mobile phase. Chromatograms were recorded by UV absorption spectroscopy at 280 nm and by fluorescence spectroscopy at λex 280 nm/λem 347 nm. The method was applied to the determination of flavan-3-ols in various cranberry samples. Catechin, epicatechin, and procyanidins A2 and B2 were found in different amounts depending on the cranberry varieties.

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Acknowledgements

The authors gratefully acknowledge the financial support received from Spanish Ministry of Economy and Competitiveness under the projects CTQ2014-65324 and CTQ2015-63968-C2-1-P, and from the Agency for Administration of University and Research Grants (Generalitat de Catalunya, Spain) under the projects 2014 SGR-377 and 2014 SGR-539.

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Authors and Affiliations

  1. Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Martí i Franquès, 1-11, 08028, Barcelona, Spain

    Inal Bakhytkyzy, Oscar Nuñez & Javier Saurina

  2. Research Institute in Food Nutrition and Food Safety, University of Barcelona, Recinte Torribera, Av. Prat de la Riba 171, Edifici de Recerca (Gaudí), Santa Coloma de Gramenet, 08921, Barcelona, Spain

    Oscar Nuñez & Javier Saurina

  3. Serra Húnter Fellow, Generalitat de Catalunya, Barcelona, Spain

    Oscar Nuñez

Authors
  1. Inal Bakhytkyzy
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  2. Oscar Nuñez
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  3. Javier Saurina
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Correspondence to Javier Saurina.

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Inal Bakhytkyzy declares that he has no conflict of interest. Oscar Nuñez declares that he has no conflict of interest. Javier Saurina declares that he has no conflict of interest.

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This article does not contain any studies with humans and animals performed by any of the authors.

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Bakhytkyzy, I., Nuñez, O. & Saurina, J. Size Exclusion Coupled to Reversed Phase Liquid Chromatography for the Characterization of Cranberry Products. Food Anal. Methods 12, 604–611 (2019). https://doi.org/10.1007/s12161-018-1390-z

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  • DOI: https://doi.org/10.1007/s12161-018-1390-z

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